Abstract:
:Given recent efforts to determine the sequence information on thousands of genes in the human genome, the current challenge is to identify the functions of these genes, including those encoding the regulator of G-protein signaling protein gene superfamily, and to establish their roles in particular signaling pathways in a native system. Increasingly, reverse genetic approaches are being used to address these questions. This article compares two powerful approaches [ribozyme and "short interfering" RNA (siRNA) techniques] under identical conditions for the first report on the suppression of endogenous RGS domain-containing RhoGEFs. The siRNA technique was found to be much more potent than ribozyme targeting at the same mRNA site of RGS-RhoGEFs. Also, the three siRNAs targeting LARG, PDZ-RhoGEF, and p115-RhoGEF are able to discriminate the closely related sequences within this RGS-RhoGEF gene family.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Wang Q,Liu M,Kozasa T,Rothestein JD,Sternweis PC,Neubig RRdoi
10.1016/S0076-6879(04)89015-3subject
Has Abstractpub_date
2004-01-01 00:00:00pages
244-65eissn
0076-6879issn
1557-7988pii
S0076687904890153journal_volume
389pub_type
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