Abstract：
:The equivalent response of evaporative light scattering detector (ELSD) for compounds of similar structure is exploited to develop an LC/ELSD method for the simultaneous quantitation of the four main components of gentamicin sulfate, using as external standard the one main component kanamycin. A C18 column was used along with a mobile phase consisting of H2O (containing 35.4 microg/ml of trichloroacetic acid and 0.89 microl/ml of trifluoroacetic acid)-methanol-acetonitrile (990:5:5, v/v/v), in an isocratic mode at 1.1 ml/min. Parameters of ELSD were 50 degrees C for evaporation temperature and 3.0 bar for pressure of carrier gas (N2). A logarithmic calibration curve was obtained for sulfate (tR = 1.9 min) from 4.2 to 150 microg/ml (r > 0.994) with a precision of 0.18%R.S.D. Kanamycin and the four gentamicin components (C(1a), C2, C(2a) and C1) were eluted at 3.2, 4.6, 5.9, 7.1 and 8.7 min, respectively, with good resolution (Rs > 1.5). Logarithmic calibration curve was obtained for each component (r > 0.99) with statistically equal slopes varying from 2.457 to 2.558. The mass range of total gentamycin was 35-240 microg/ml. The proposed method was applied for the determination of gentamicin components and sulfate in raw materials and pharmaceutical formulations (injection, drops and cream) without any pretreatment except cream, for which liquid-liquid extraction was required. Recovery from standard addition experiments in commercial formulations was 99-100% regarding total gentamicin and 89-108% regarding individual components, with a precision (%RSD, n = 4) 0.7-5.8%.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Megoulas NC,Koupparis MAdoi
10.1016/j.jpba.2004.05.018subject
Has Abstractpub_date
2004-09-21 00:00:00pages
73-9issue
1eissn
0731-7085issn
1873-264Xpii
S0731708504002468journal_volume
36pub_type
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