A simple method to monitor serum concentrations of fluoxetine and its major metabolite for pharmacokinetic studies.

abstract：:Tobramycin is one of a class of aminoglycoside antibiotics that lack a good chromophore, and is therefore difficult to determine using reversed-phase HPLC with absorbance detection. This is especially true for determining the quantity of each impurity. We show that tobramycin and its major impurities, including kanamy...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Hanko VP,Rohrer JS

更新日期：2006-03-03 00:00:00

abstract：:A combined method of immunoaffinity extraction with high-performance liquid chromatography has been developed for the enantioselective determination of bufuralol and its metabolites in human plasma. The antibodies having high affinity toward the asymmetric center at the C-1 position of bufuralol and its 1'-oxidized me...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Ikegawa S,Matsuura K,Sato T,Isriyanthi NM,Niwa T,Miyairi S,Takashina H,Kawashima Y,Goto J

更新日期：1998-05-01 00:00:00

abstract：:Concentrated solutions of doxycycline are often added to drinking water of animals for oral antibiotic therapy. However, stability concerns of doxycycline in solution involve an accurate selection of the solvent system to ensure that the active substance will remain within the acceptance range during the product shelf...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Jutglar M,Foradada M,Caballero F,Hoogmartens J,Adams E

更新日期：2018-09-10 00:00:00

abstract：:The aim of this study was to evaluate the relationship between serum levels of beta-trace protein (BTP), a low molecular weight (MW) protein, and glomerular filtration rate (GFR). GFR and serum levels of BTP, and for comparison creatinine (Creat), cystatin C (Cys) and beta 2-microglobulin (beta 2M), were measured in 6...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Donadio C,Lucchesi A,Ardini M,Donadio E,Giordani R

更新日期：2003-08-08 00:00:00

abstract：:Cloud-point extraction (CPE) is attracting increasing interest in a number of analytical fields, including bioanalysis, as it provides a simple, safe and environmentally-friendly sample preparation technique. However, there are only few reports on the application of this extraction technique in liquid chromatography-e...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Giebułtowicz J,Kojro G,Piotrowski R,Kułakowski P,Wroczyński P

更新日期：2016-09-05 00:00:00

abstract：:Enantiomers of clenbuterol were directly separated by a new high performance chromatographic method on Chirex 3005 column. Several parameters such as mobile phase composition, column temperature and flow rate were studied. Baseline enantioseparation was achieved, using the optimized mobile phase of n-hexane-1,2-dichol...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Song Y,Wang D,Hu Y,Chen X,Jiao Y,Hou D

更新日期：2003-02-26 00:00:00

abstract：:A methylene blue (MB) poly(vinyl chloride) membrane sensor based on MB-silicotungstate (SLT) ion association as electroactive material was described. The linear response covered the range 1 x 10(-3)-1 x 10(-6) mol x dm(-3) MB solution, with a slope 52.0+/-0.8 mV decade(-1) (pH range 3.0-10.0). The detection limit was ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Wen ML,Zhao YB,Chen X,Wang CY

更新日期：1999-01-01 00:00:00

abstract：:In this study, a green production method was used to obtain magnetic carbon nanodot/graphene oxide hybrid material (Fe3O4@C-nanodot@GO) for the magnetic solid phase extraction (MSPE) of ibuprofen (IBU) in human plasma prior to HPLC-DAD determination. For the first time in the literature, Fe3O4@C-nanodot@GO hybrid mate...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Yuvali D,Narin I,Soylak M,Yilmaz E

更新日期：2020-02-05 00:00:00

abstract：:This work deals with the preparation of single-labeled bioconjugates of the antibiotic Gentamicin (GT) with the sulforhodamine-derived fluorescence dye Texas Red(®)-X (TR), its purification by high-performance liquid chromatography (HPLC) and its characterization by high-resolution mass spectrometry. Aminoglycosides s...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Woiwode U,Sievers-Engler A,Lämmerhofer M

更新日期：2016-03-20 00:00:00

abstract：:Surface plasmon resonance (SPR) is a promising tool in sensor technology for biomedical applications. An SPR based immunosensor was established for label free and real time assay for the serological diagnosis of dengue virus infection employing the dengue virus antigen as the sensing element. The dengue virus antigen ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Kumbhat S,Sharma K,Gehlot R,Solanki A,Joshi V

更新日期：2010-06-05 00:00:00

abstract：:S-Adenosyl-L-methionine (SAM) and its metabolites S-adenosyl-L-homocysteine (SAH) and methyl-thioadenosine (MTA) are endogenous compounds that are heavily involved in a variety of biochemical processes, and have therefore been the target for several assays in body fluids and tissues. Reversed-phase chromatographic beh...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Valkó K,Hamedani MP,Ascah TL,Gibbons WA

更新日期：1993-04-01 00:00:00

abstract：:The mixture of creatinine, activated charcoal and water was stirred. As a result the conversion of creatinine into two products was observed. (1)H, (13)C NMR and HMBC spectra were recorded and the chemical shifts assigned. Two uremic toxins: creatol and N-methylguanidine were identified. To interpret the NMR data obta...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Krawczyk H

更新日期：2009-05-01 00:00:00

abstract：:Capillary zone electrophoresis is a technique that is rapidly gaining popularity in the pharmaceutical industry because of the wide range of compounds applicable to the technique. These range from small biologically active molecules through to large nucleotides and proteins. This review outlines the historical develop...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Smith NW,Evans MB

更新日期：1994-05-01 00:00:00

abstract：:The phenolic composition of herbal tea (HT) and hydromethanolic extract (HME) obtained from Grindelia robusta Nutt. was studied by HPLC-DAD-ESI/MS(n). Thirty six flavonoids and hydroxycinnamic acids were detected, from which thirty are described for the first time in this species. Quantification by HPLC-DAD showed tha...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Ferreres F,Grosso C,Gil-Izquierdo A,Valentão P,Azevedo C,Andrade PB

更新日期：2014-06-01 00:00:00

abstract：:Health benefits of wine, especially with red wine, have been linked to the presence of a wide range of phenolic antioxidants. Thus, the aim of this study was to develop a simple, high performance thin layer chromatographic (HPTLC) method combined with high resolution digital plate images to visually compare multiple w...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Agatonovic-Kustrin S,Hettiarachchi CG,Morton DW,Razic S

更新日期：2015-01-01 00:00:00

abstract：:A field-amplified sample stacking (FASS) and capillary zone electrophoresis (CZE) method is described for the quantification of ambroxol hydrochloride in human plasma, following liquid-liquid extraction in the 96-well format. The separation was carried out at 25 °C in a 31.2 cm × 75 μm fused-silica capillary with an a...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Li J,Bi Y,Wang L,Sun F,Chen Z,Xu G,Fan G

更新日期：2012-07-01 00:00:00

abstract：:A method using reverse phase liquid chromatography-tandem mass spectrometry and cassette administration was developed for in vivo brain and plasma exposure profiling to assist early CNS drug discovery programs. Three to four compounds were grouped in cassettes for dosing and analysis. Compounds in the cassettes were s...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Zhang MY,Kerns E,McConnell O,Sonnenberg-Reines J,Zaleska MM,Steven Jacobsen J,Butera J,Kreft A

更新日期：2004-02-04 00:00:00

abstract：:Both a GC-MS and an LC method have been developed for the simultaneous quantitation of dolasetron and reduced dolasetron in human plasma. The GC-MS method has been utilized in preliminary human pharmacokinetic studies of dolasetron mesylate. Selected ion monitoring was used in these initial studies to obtain the sensi...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Gillespie TA,Eckstein JA,Nardella P,Coutant JE

更新日期：1993-10-01 00:00:00

abstract：:Nanoformulations are complex systems where physicochemical properties determine their therapeutic efficacy and safety. In the case of nanovaccines, particle size and shape play a crucial role on the immune response generated. Furthermore, the antigen's integrity is also a key aspect to control when producing a nanovac...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Klein M,Menta M,Dacoba TG,Crecente-Campo J,Alonso MJ,Dupin D,Loinaz I,Grassl B,Séby F

更新日期：2020-02-05 00:00:00

abstract：:This report describes the fabrication and the application of a novel carbon nanotube (CNT)-epoxy composite electrode as a sensitive amperometric detector for the capillary electrophoresis (CE). The composite electrode was fabricated on the basis of the in situ polycondensation of a mixture of CNTs and 1,2-ethanediamin...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Zhang L,Zhang W,Chen G

更新日期：2015-11-10 00:00:00

abstract：:A high throughput method was developed and validated for the quantitative determination of LAG078, a lipid-lowering compound, in dog plasma obtained during toxicokinetic studies. The method was based on reverse phase liquid chromatographic separation of the analyte from plasma extract followed by turbo-ionspray (TIS) ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Majumdar TK,Wu S,Vedananda S,Tse FL

更新日期：2004-06-29 00:00:00

abstract：:Macrolides are multicomponent drugs whose impurity control is always a challenge demanding analysis method with good sensitivity and selectivity. Three separate, sensitive, accurate liquid chromatography tandem mass spectrometry methods (LC-MS) were developed for the measurement of three 16-membered ring macrolides (j...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Zhang X,Li J,Wang C,Song D,Hu C

更新日期：2017-10-25 00:00:00

abstract：:Dermal microdialysis (DMD) is a technique capable of determining the percutaneous penetration of drugs from topical formulations intended for local and/or regional activity. Typically, the concentrations of drug collected in dialysates are very low, generally in the ng/ml or even pg/ml range. An additional challenge i...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Tettey-Amlalo RN,Kanfer I

更新日期：2009-11-01 00:00:00

abstract：:Instrumentation for the concurrent, dynamic monitoring of active sweat glands and perspiration volume is described. A device for the measurement of the rate of sweat secretion was installed on the head part of a microscope. The combined apparatus (microscopic probe) is handy for use and its weight is very light (ca. 3...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Kamei T,Naitoh K,Nakashima K,Ohhashi T,Kitagawa S,Tsuda T

更新日期：1997-06-01 00:00:00

abstract：:Docetaxel is a semi-synthetic taxane with cytotoxic anti-neoplastic activity and, currently used as anticancer agent in several types of cancer. Docetaxel is highly bound to plasma proteins, and this significantly determines its clearance and activity. Therefore, measurement of free docetaxel in plasma is pharmacologi...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Rigo-Bonnin R,Cobo-Sacristán S,Gonzalo-Diego N,Colom H,Muñoz-Sánchez C,Urruticoechea A,Falo C,Alía P

更新日期：2016-01-05 00:00:00

abstract：:Flavors are commonly used in pharmaceutical oral solutions and oral suspensions to mask drug bitterness and to make the formulation more palatable. Flavor analysis during product development is typically performed by human organoleptic analysis, which is often expensive and less objective. A novel approach using a met...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Zhu L,Seburg RA,Tsai E,Puech S,Mifsud JC

更新日期：2004-02-18 00:00:00

abstract：:The objective of this study was to investigate the use of transmission hard X-ray nano-computed-tomography (nano-CT) for characterization of the pore structure and drug distribution in poly (lactic-co-glycolic acid) (PLGA) microspheres encapsulating bovine serum albumin and to study the correlation between drug distri...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Huang X,Li N,Wang D,Luo Y,Wu Z,Guo Z,Jin Q,Liu Z,Huang Y,Zhang Y,Wu C

更新日期：2015-08-10 00:00:00

abstract：:Azurin p28 (NSC745104) is a 28 amino acid peptide fragment that inhibits proliferation of human solid and hematological malignancies in vitro and in vivo by reducing proteasomal degradation of oncogene p53. The present study aimed at developing a novel and fast liquid chromatography/tandem mass spectrometry (LC/MS/MS)...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Gorman GS,Coward LU,Freeman L,Noker PE,Beattie CW,Jia L

更新日期：2010-12-01 00:00:00

abstract：:Growth media for mammalian cell culture are very complex mixtures of several dozens of ingredients, and thus the preparation of qualified media is critical to viable cell density and final product titers. For liquid media prepared from powdered ingredients, sterile filtration is required prior to use to safeguard the ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Cao X,Loussaert JA,Wen ZQ

更新日期：2016-02-05 00:00:00

abstract：:A high performance liquid chromatographic method was developed to simultaneously determine the 12 major constituents of Forsythia suspensa, namely R-suspensaside, S-suspensaside, S-suspensaside methyl ether, (+)-pinoresinol-beta-d-glucoside, forsythiaside, (+)-epipinoresinol-4'-O-glucoside, suspensaside A, rutin, phil...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Guo H,Liu AH,Li L,Guo DA

更新日期：2007-02-19 00:00:00