Abstract：
INTRODUCTION:Collected and archived serum samples could be important sources for genetic studies, once DNA suitable for molecular genetic studies could be obtained from them. METHODS:DNA was isolated from 54 archived sera samples, collected previously from the participants of a Hungarian allergy study, with commercially available isolation kit. The authors have determined the concentration of the isolated DNA (81.88 +/- 52.36 ng/ml) and the size of the isolated fragments was estimated using semiquantitative real-time PCR. Two primers were used producing two different fragment size, for the phospholipase 2A and the actin beta genes, and melting curve analyses was performed as quality control. RESULTS:The concentration of the phospholipase 2A product was 2.9798 +/- 5.4454 microg/microl and the actin beta gene was 0.0015 +/- 0.0011 microg/microl. The melting curve analysis served as a quality control for the determination of the size of PCR products. In the case of the phospholipase 2A all samples produced the 133 bp PCR fragments, except one, while in the case of actin beta gene only six sample showed the expected 178 bp product, all the others samples had smaller fragments. CONCLUSIONS:These results confirm the suitability of the DNA isolated from archived sera samples for further molecular biological studies (SNP analysis, mutation detection) and give an estimate for the product size of the isolated DNA. Sera samples have been collected years ago can be a good source of genetic information on different diseases.
journal_name
Orv Hetiljournal_title
Orvosi hetilapauthors
Nagy B,Tarja L,Bán Z,Andrásofszky Z,Papp G,Lázár L,Nékám K,Papp Z,Réthy LAsubject
Has Abstractpub_date
2004-11-21 00:00:00pages
2375-8issue
47eissn
0030-6002issn
1788-6120journal_volume
145pub_type
杂志文章相关文献
ORVOSI HETILAP文献大全abstract：:Nijmegen Breakage syndrome is a rare, autosomal recessive disorder characterized by severe, combined immunodeficiency, recurrent sinopulmonary infections, chromosomal instability, radiosensitivity, predisposition to malignancy, a "bird-like" facial appearance, progressive microcephaly, short stature, and mental retard...
journal_title：Orvosi hetilap
pub_type： 杂志文章,评审
doi：10.1556/OH.2010.28851
更新日期：2010-04-18 00:00:00
abstract：:Aim of the present work was the development of a mechanic cell separation protocol for gastrointestinal biopsy analysis. Evaluation of the technique was performed on selected group of patients who underwent routine endoscopy. Routine gastrointestinal biopsies were obtained after informed consent. 23 gastric (6 healthy...
journal_title：Orvosi hetilap
pub_type： 杂志文章
doi：
更新日期：2000-04-09 00:00:00
abstract：:Chronic obstructive pulmonary disease is often associated with cardiovascular diseases, such as pulmonary hypertension, ischemic heart disease, arrhythmias or heart failure. These co-morbidities may cause diagnostic or therapeutic difficulties and significantly worsen the morbidity and mortality of patients with chron...
journal_title：Orvosi hetilap
pub_type： 杂志文章,评审
doi：10.1556/OH.2014.29989
更新日期：2014-09-14 00:00:00
abstract：INTRODUCTION:The phenomenon of stress induced left ventricular dysfunction has been long recognised. A special reversible form of it, characterized by left ventricular apical dilatation, is the so-called "tako-tsubo" or ampoule cardiomyopathy, based on its first description by Sato et al. in 1990. The tako-tsubo cardio...
journal_title：Orvosi hetilap
pub_type： 杂志文章
doi：10.1556/OH.2008.28181
更新日期：2008-02-24 00:00:00
abstract：:110 urgent sonography were performed on 101 patients with suspected acute appendicitis and on subsequent 26 patients 33 echography with suspected postappendectomy abscess. Although according to a few publications in connection with acute appendicitis there are characteristic sonographic signs, the authors didn't find ...
journal_title：Orvosi hetilap
pub_type： 杂志文章,评审
doi：
更新日期：1989-04-16 00:00:00
abstract：:Various types of fish-poisoning mainly occur in tropical areas. Saxitoxin, tetrodotoxin, scombrotoxin and clupeotoxin are heat labile while ciguatoxin, in contrast, is heat stable thus it may be the source of severe poisoning even in case of thorough cooking and baking. In December 1987 a mass alimentary poisoning occ...
journal_title：Orvosi hetilap
pub_type： 杂志文章
doi：
更新日期：1990-10-07 00:00:00
abstract：:Critical-care physicians are facing a challenging process in healthcare due to the overwhelming case number of hypoxic respiratory failure patients. Pneumonia has an utmost importance in the primary pathomechanism of the development of critical illness in the COVID-19 patients. Thus, imaging techniques are situated in...
journal_title：Orvosi hetilap
pub_type： 杂志文章,评审
doi：10.1556/650.2020.31814
更新日期：2020-04-01 00:00:00
abstract：:The well-known psychoactive drug widely used in the daily clinical praxis, the piracetam has many therapeutical indications. The drug is extensively applied in the therapy of ischaemic stroke, aphasy as well as of dementias of several origins and age-depending cognitive disturbances. One of the most important effect o...
journal_title：Orvosi hetilap
pub_type： 杂志文章,评审
doi：
更新日期：2002-05-19 00:00:00
abstract：:Over recent years the clinical importance of invasive fungal infections has significantly increased. Mucormycosis refers to a rare, life threatening, opportunistic disease caused by filamentous fungi of the order Mucorales. Most frequently mucormycosis occurs in patients with ketoacidotic diabetes and in neutropenic p...
journal_title：Orvosi hetilap
pub_type： 杂志文章
doi：
更新日期：2004-12-12 00:00:00
abstract：:In many countries osteoporosis is the most common metabolic bone diseases. A great deal is known about the pathophysiology and the treatment of the disease. There is a lot of treatment possibilities and many new treatments are being tested. Therapeutic agents for osteoporosis are correspondingly classified as substanc...
journal_title：Orvosi hetilap
pub_type： 杂志文章,评审
doi：
更新日期：1999-09-19 00:00:00
abstract：:After a short overview of the history of probiotics, the author presents the development of human intestinal microflora based on the newest genetic data and the microbiological features of main probiotics. The indications of probiotic administration have been defined and extended in recent years. The author reviews si...
journal_title：Orvosi hetilap
pub_type： 杂志文章,评审
doi：10.1556/OH.2013.29547
更新日期：2013-02-24 00:00:00
abstract：:A total of 171 pleural needle biopsies were carried out in 123 patients with pleural effusion. The sensitivity of the method proved to be 51% for malignant diseases and 75% for tuberculotic pleurisy, respectively. Performing biopsy simultaneously with the cytological examination of the pleural fluid, malignancy was pr...
journal_title：Orvosi hetilap
pub_type： 杂志文章
doi：
更新日期：1993-11-14 00:00:00
abstract：:The results of seven gonadotropin releasing hormone (GnRH) loading tests are presented with special respect to the changes of melatonin level. The tests were carried out after a withdrawal bleeding triggered by progesterone (100 mg for 5 days) and plasma FSH, LH, prolactin and melatonin level were determined. The init...
journal_title：Orvosi hetilap
pub_type： 杂志文章
doi：
更新日期：1992-08-23 00:00:00
abstract：:Complications of chronic liver diseases requiring surgical interventions are the following: esophageal varices and bleeding, ascites hypersplenism, some forms of liver insufficiency. The most widely accepted method for treatment of esophageal varices is endoscopic sclerotherapy. Portal systemic shunts are indicated in...
journal_title：Orvosi hetilap
pub_type： 杂志文章,评审
doi：
更新日期：1992-08-09 00:00:00
abstract：INTRODUCTION:Healthcare associated infections and antimicrobial use are common among residents of long-term care facilities. Faced to the lack of standardized data, the European Centre for Disease Prevention and Control funded a project with the aim of estimating prevalence of infections and antibiotic use in European ...
journal_title：Orvosi hetilap
pub_type： 杂志文章
doi：10.1556/OH.2014.29876
更新日期：2014-06-08 00:00:00
abstract：:In nonselected patients 81.5% stone-free rate could be reached in 3 months. In authors's opinion Direx Tripter X-1 is suitable in the treatment of renal and ureteral calculi. They treated staghorn calculi by ESWL monotherapy with success. The Direx lithotripter was found to be effective and easy to operate; the potent...
journal_title：Orvosi hetilap
pub_type： 杂志文章
doi：
更新日期：1992-08-16 00:00:00
abstract：:This paper describes a case of an epidermoid cyst arising in the anterior wall of the coecum. Epidermoid and dermoid cysts of the coecum are exceptionally rare. These cysts arise by the sequestration of squamous epithelium during embryological development, or following trauma or surgery. The true cysts are the result ...
journal_title：Orvosi hetilap
pub_type： 杂志文章
doi：
更新日期：2001-07-22 00:00:00
abstract：INTRODUCTION:Colorectal cancer is the second most common cause of cancer related death in Hungary. AIM:The authors examined retrospectively the ratio and the clinical course of patients under the age of 50 years operated by themselves between January, 2006 and May, 2012. METHOD:During this period of time 667 patients...
journal_title：Orvosi hetilap
pub_type： 杂志文章
doi：10.1556/OH.2013.29687
更新日期：2013-08-25 00:00:00
abstract：:Chronic pancreatitis is a persistent inflammatory disorder of the pancreas, characterized by destruction of the pancreatic parenchyma, maldigestion, chronic pain and diabetes mellitus. Genetic factors determining susceptibility to chronic pancreatitis can be classified in two groups: 1. rare gene mutations affecting t...
journal_title：Orvosi hetilap
pub_type： 杂志文章,评审
doi：10.1556/OH.2008.28441
更新日期：2008-09-07 00:00:00
abstract：:The goal of stereotactic radiosurgery by definition is "the delivery of a single, high dose of radiation allowing the precise and complete destruction of chosen target structures containing healthy and/or pathological cells, without significant concomitant or late radiation damage to adjacent tissues". This effect is ...
journal_title：Orvosi hetilap
pub_type： 杂志文章,评审
doi：
更新日期：2001-10-07 00:00:00
abstract：:Non alcoholic fatty liver disease is the hepatic manifestation of metabolic syndrome, and the most common liver disease. Its more aggressive form is the non alcoholic steatohepatitis. Multiple genetic and environmental factors lead to the accumulation of triglicerides and the inflammatory cascade. High fat diet, obesi...
journal_title：Orvosi hetilap
pub_type： 杂志文章,评审
doi：10.1556/650.2017.30775
更新日期：2017-06-01 00:00:00
abstract：:From July 1, 1994 to July 1, 1997 a total of 17 children with Chiari I malformation diagnosed by MRI was seen in our hospital. Six of them underwent craniocervical decompression and tonsilar resection. The major complaints of all these children were gait ataxia and persisting (longer than 6 month) headache, and one ch...
journal_title：Orvosi hetilap
pub_type： 杂志文章
doi：
更新日期：2000-03-12 00:00:00
abstract：:15 cases of subacute cutaneous lupus erythematosus are reported. The diagnosis was based on the presence of the typical clinical features, on the histologic and immunpathologic examination of lesional skin and on the characteristic laboratory findings. 8 patients had annular type, 4 patients had papulosquamosus type o...
journal_title：Orvosi hetilap
pub_type： 杂志文章,评审
doi：
更新日期：1989-12-03 00:00:00
abstract：:Low HDL cholesterol level is an independent risk factor in the cardivascular diseases and the therapeutic aim is to increase this. In high risk patients (cardiovascular disease-, diabetic- or metabolic syndrome patients), the target value of HDL cholesterol is more than 1,0 mmol/l in males and 1,3 mmol/l in females. T...
journal_title：Orvosi hetilap
pub_type： 杂志文章,评审
doi：
更新日期：2006-11-26 00:00:00
abstract：:The authors detected Epstein-Barr virus (EBV) virus capsid (VCA) antibodies of IgM and IgG type together with EBNA and heterophil antibodies in the sera collected from 1882 unselected patients without infectious mononucleosis. Out of them IgM type antibodies were found in 123 cases and in an additional group of 30 pat...
journal_title：Orvosi hetilap
pub_type： 杂志文章
doi：
更新日期：1996-07-28 00:00:00
abstract：PATIENT AND METHODS:174 patients who had cardiac adenocarcinoma were examined in the endoscopic laboratory of the Surgical Department between March 1, 1994 and October 29, 2002. In the course of the endoscopic examinations intramural metastases were found in 19 patients (10.9%). Of the patients with esophageal metastas...
journal_title：Orvosi hetilap
pub_type： 杂志文章
doi：
更新日期：2004-01-18 00:00:00
abstract：:Anabolic steroids and creatine supplementation is one of the current abuse used by body builders. It is less known that this combination beside of many deleterious effects may also cause renal damage. Authors report a case of diffuse membranoproliferative glomerulonephritis type I in a 22-year-old man who had been tak...
journal_title：Orvosi hetilap
pub_type： 杂志文章
doi：
更新日期：2003-12-07 00:00:00
abstract：INTRODUCTION:The internationalization of societies and the processes of globalization raise new challenges towards health provision systems, including professional, ethical, social, cultural and communicational references of nursing and nursing education. AIMS:In this study the authors wanted to answer the following q...
journal_title：Orvosi hetilap
pub_type： 杂志文章
doi：10.1556/OH.2012.29479
更新日期：2012-10-28 00:00:00
abstract：:Polycystic ovary syndrome is recognized as the most common hormonal and metabolic disorder of women. This heterogeneous endocrinopathy characterized by clinical and/or biochemical hyperandrogenism, oligo- or amenorrhoea, anovulatory infertility, and polycystic ovarian morphology. The prevalence, clinical feature and t...
journal_title：Orvosi hetilap
pub_type： 杂志文章,评审
doi：10.1556/OH.2014.29941
更新日期：2014-07-27 00:00:00
abstract：:The chronic recurrent multifocal osteomyelitis has been reported very rarely in the literature. However, its significance must be emphasized, because it is a spontaneously healing, benign disease, as compared to the classical forms of osteomyelitis. It leaves behind almost no residual symptoms, and many operations, lo...
journal_title：Orvosi hetilap
pub_type： 杂志文章
doi：
更新日期：2003-12-21 00:00:00
