Abstract:
BACKGROUND:There is growing interest in new therapeutic options for the treatment of end-stage liver diseases. In addition to mechanical devices supporting liver function, such as bioreactors, the transplantation of hepatocyte-like cells derived from (adult) stem cells offer great perspectives. We have generated hepatocyte-like (NeoHep) cells from terminally differentiated peripheral blood monocytes and, in this study, have evaluated these cells as a possible tool for autologous cell therapy. METHODS:Peripheral blood monocytes were cultured under conditions that promote hepatocyte-like differentiation and were characterized for hepatocyte marker expression by reverse-transcriptase polymerase chain reaction, immunohistochemistry, and immunoblotting and for specific secretory and metabolic functions with the appropriate biochemical assays. RESULTS:NeoHep cells resembled primary human hepatocytes with respect to morphology, expression of hepatocyte markers (albumin, cytochrome P450 isoenzymes, asialoglycoprotein receptor, coagulation factor VII), various secretory and metabolic functions (albumin secretion, urea production, lactate formation, and lactate dehydrogenase and aspartate transaminase release), and drug detoxification activities (phase I metabolization of ethoxycoumarin into 7OH-coumarin after stimulation with 3-methylcholanthren, induction of CYP3A4 activity, and phase II metabolization through UDP-glucuronidation of 4-methyl-umbelliferone). CONCLUSIONS:These data convincingly show that NeoHep cells display a phenotype and specific in vitro metabolic functions that are quantitatively and qualitatively comparable in part with those of primary human hepatocytes. These cells could thus be clinically applied in an autologous setting for the treatment of end-stage liver diseases or for improving liver function in patients who have undergone critical liver-mass resection.
journal_name
Transplantationjournal_title
Transplantationauthors
Ruhnke M,Nussler AK,Ungefroren H,Hengstler JG,Kremer B,Hoeckh W,Gottwald T,Heeckt P,Fandrich Fdoi
10.1097/01.tp.0000157362.91322.82subject
Has Abstractpub_date
2005-05-15 00:00:00pages
1097-103issue
9eissn
0041-1337issn
1534-6080pii
00007890-200505150-00026journal_volume
79pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract:BACKGROUND:Controversy exists about the conditions effecting the development of forkhead/winghead helix transcription factor P3 (FOXP3) expressing T cells and their relevance in transplant recipients. METHODS:We generated carboxy-fluorescein diacetate succinimidyl ester-labeled CD4+CD25 high FOXP3+ cells in mixed lymp...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181bbee98
更新日期:2009-12-15 00:00:00
abstract:BACKGROUND:We have previously shown that phosphorothioate intercellular adhesion molecule (ICAM)-1 antisense oligodeoxynucleotide (oligo) IP-9125 blocks the expression of rat ICAM-1 mRNA in rat L2 cells. A single ex situ perfusion of grafts with unformulated IP-9125, suspended in Euro-Collins solution, prolonged the su...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199909270-00022
更新日期:1999-09-27 00:00:00
abstract::Cyclosporine (CsA) administration and nitric oxide (NO) blockade promote similar chronic renal hemodynamic alterations in rats. We evaluated various clinical CsA doses under conditions of NO blockade using L-NAME (N-nitro L-arginine methyl ester). Groups of Sprague-Dawley rats kept on a normal salt (+NaCl) or low-salt...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199605270-00017
更新日期:1996-05-27 00:00:00
abstract::Modified limiting dilution analysis (LDA) techniques were used to evaluate the mobilization of antigen-stimulated helper T lymphocytes (HTL) and cytotoxic T lymphocytes (CTL) following allogeneic heterotopic cardiac transplantation. These modified LDA techniques allow a quantitative comparison of T cells that have bee...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::The overall incidence of malignancy in renal transplant recipients is 100-fold higher compared with age matched controls. Routine clinical evaluation therefore often includes the determination of serum tumor markers AFP, CA19-9, CEA, CA125, CA15-3, PSA, and calcitonin. We evaluated the specificity and the sensitivity ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199611270-00021
更新日期:1996-11-27 00:00:00
abstract::We have previously demonstrated that the placental spongiotrophoblast and yolk sac venous plexus express paternally derived H-2K and D (class I) antigens in a manner accessible to maternal circulation, and that the placenta serves to prevent antipaternal antibodies from reaching the fetus. Kinetic studies indicated th...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198403000-00016
更新日期:1984-03-01 00:00:00
abstract:BACKGROUND:Localization of inflammatory stimuli may direct lung allografts to different phenotypes of chronic dysfunction, such as bronchiolitis obliterans syndrome (BOS) or restrictive allograft syndrome (RAS). We hypothesized that airway stimulation with lipopolysaccharide (LPS) in rats leads to airway-centered infla...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000003097
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND:The authors studied the relationship between the dynamics of Epstein-Barr virus (EBV) load, CD8 T-cell activation and differentiation, and EBV-associated symptoms in 25 children after kidney transplantation (Tx). METHODS:Twenty-two patients were enrolled at the time of Tx and three at diagnosis of EBV-induc...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000131168.21930.5c
更新日期:2004-06-15 00:00:00
abstract:BACKGROUND:The infiltration of epithelium by CD8+ T lymphocytes in human renal or liver allografts is a critical feature of acute rejection. CD103 expression can be acquired in vitro by CD8+ cytotoxic T lymphocytes in response to allogeneic renal epithelial cells and promotes their adhesion to epithelium and subsequent...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:2000-07-15 00:00:00
abstract::Cell-mediated cytotoxicity directed toward pancreatic islets plays a significant role in the pathogenesis of type 1 diabetes mellitus. Histologically, autoimmune insulitis appears as mononuclear infiltrates that precede actual beta cell loss. Experimental studies by Bartlett et al. on the BB rat model suggest that who...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199612270-00014
更新日期:1996-12-27 00:00:00
abstract:BACKGROUND:Preexisting IgG antibodies to donor human leukocyte antigens (HLA) are a risk factor for rapid allograft rejection. However, non-HLA antibodies, of the IgM class, also called autoreactive antibodies, are not believed to affect graft survival. The aim of this study was to determine the incidence and clinical ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31819a65fa
更新日期:2009-03-27 00:00:00
abstract:BACKGROUND:Cold preservation is the most practical method to maintain the viability of isolated lungs. However, rapid cooling may affect pulmonary endothelial function. We examined the effects of microtubule stabilization with paclitaxel on pulmonary endothelial barrier integrity under cold temperature. METHODS:Human ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000131951.72851.57
更新日期:2004-08-27 00:00:00
abstract::Posttransplant lymphoproliferative disorders (PTLD) are EBV-associated lymphoid neoplasms that are caused by the uncontrolled growth of EBV-infected B lymphocytes. The clinical presentation of PTLD can range from benign polygonal lymphoproliferative disorders to aggressive monoclonal immunoblastic lymphomas. In this r...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199606270-00010
更新日期:1996-06-27 00:00:00
abstract::We have proposed that the mechanisms of alloengraftment and variable acquired tolerance can be facilitated by minimum posttransplant immunosuppression. It was further suggested that the efficacy of minimalistic treatment could be enhanced by preoperative recipient conditioning with an antilymphoid antibody preparation...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000142674.78268.01
更新日期:2004-10-15 00:00:00
abstract::Murine lymphoid cells sensitized against allografts express two types of cytotoxicity measured in vitro in short-term assays of 2 to 3 hr by 51Cr release. One type of cytotoxicity is specific against target cells carrying the sensitizing alloantigens, and the other type is nonspecific against both syngeneic and unrela...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197602000-00002
更新日期:1976-02-01 00:00:00
abstract:BACKGROUND:Although islet xenotransplantation is a promising therapy for type 1 diabetes, its clinical application has been hampered by cellular rejection and the requirement for high levels of immunosuppression. The aim of this study was to determine the role of Foxp3 regulatory T (Treg) cells in costimulation blockad...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000001482
更新日期:2017-07-01 00:00:00
abstract:BACKGROUND:It is unclear whether ischemic preconditioning (IPC) of solid organs induces remote IPC (RIPC) in donors after brain death (DBD). METHODS:Outcomes in kidney recipients from 163 DBD in two randomized trials of liver IPC (5 min=62 and 10 min=101) were obtained retrospectively from the Scientific Registry of T...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000436926.30897.56
更新日期:2014-02-15 00:00:00
abstract:OBJECTIVES:Recent studies demonstrated that prolactin (PRL) has beneficial effects on beta cells for islet transplantation. We examined the effect of human recombinant PRL (rhPRL) supplementation to the culture media to determine its potential use in the context of clinical islet transplantation. MATERIALS AND METHODS...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181d98af1
更新日期:2010-06-15 00:00:00
abstract:BACKGROUND:Based on the excellent correlation between cyclosporine A 2-hr postdose blood levels (C2) and the area under the concentration versus time curve, we evaluated the clinical benefit of Neoral dose monitoring with C2 compared trough levels (C0) in stable heart transplant patients. METHODS:We studied 114 stable...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199912270-00003
更新日期:1999-12-27 00:00:00
abstract::Clearance studies were performed in 32 transplanted children treated with CsA in combination with low-dose prednisolone (CsA group), and the results were compared with those of 29 children transplanted earlier and treated with azathioprine and prednisolone (CIS group). Serum creatinine and urea levels 6 weeks and 1 ye...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198704000-00006
更新日期:1987-04-01 00:00:00
abstract:BACKGROUND:Hyperacute rejection (HAR) of pig-to-primate discordant xenografts is caused by the deposition of preexisting natural antibodies that recognize Galalpha1-3Gal (alphaGal)-terminating oligosaccharides on glycoproteins and glycolipids, followed by complement-mediated lysis of the graft's endothelium. In vitro, ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199802150-00009
更新日期:1998-02-15 00:00:00
abstract::Retrospective mixed leukocyte culture studies were performed in eight patients who had either rejected their marrow grafts or experienced graft-versus-host disease and in seven patients with successful marrow engraftment. In tests of pre-and post-transplant lymphocytes we found no evidence that rejection or graft-vers...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197611000-00010
更新日期:1976-11-01 00:00:00
abstract::We have previously demonstrated that subclinical chronic rejection (CR) induces structural and functional alterations in enteric smooth muscle and nerves in a rat model of small intestinal transplantation. This study was designed to investigate the effect of prolonged FK506 rescue therapy on these sequelae of CR. Immu...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1995-01-27 00:00:00
abstract::We have previously shown that trophic factor supplementation (TFS) of University of Wisconsin (UW) solution enhanced kidney viability after cold storage. Here, we use an in vitro model to study the effect of TFS on early apoptotic changes after cold ischemic storage. Mitochondrial membrane potential was determined by ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000242524.35562.4b
更新日期:2007-01-15 00:00:00
abstract:BACKGROUND:Current histological methods cannot accurately determine the survival rate of human pancreatic islets following portal vein infusion. This is due, in part, to the low number of infused islets relative to the whole liver. In this study, we assessed the ability of confocal laser scanning microscopy (CLSM) to t...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000003191
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND:The long-term outcome of simultaneous kidney pancreas transplant recipients is not well established. METHODS:We retrospectively reviewed all patients who underwent simultaneous kidney-pancreas transplantation with bladder drainage at our center between January 1989 and December 1991. A total of 57 patients ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200002270-00015
更新日期:2000-02-27 00:00:00
abstract::Cyclosporine treatment of BALB/c mice (at a dose of 20 mg/kg every other day for 3 weeks) caused a remarkable reduction in the PNA-, L3T4+Lyt-2- subset of thymocytes. A significant reduction of the L3T4+Lyt-2-subset was also observed in both the lymph node and spleen cells of CsA-treated mice, though the degree of the...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198903000-00020
更新日期:1989-03-01 00:00:00
abstract:BACKGROUND:Renal transplant recipients have an increased incidence of cardiovascular disease, but less data exist about cerebrovascular atherosclerosis. In this study, we assessed the prevalence of carotid lesions as evaluated by B-mode ultrasonography in a group of renal transplant recipients, and we evaluated univari...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199902150-00005
更新日期:1999-02-15 00:00:00
abstract:BACKGROUND:The urinary CXC chemokine ligand (CXCL)10 detects renal transplant inflammation noninvasively, but has limited sensitivity and specificity. In this study, we performed urinary proteomic analysis to identify novel biomarkers that may improve the diagnostic performance of urinary CXCL10 for detecting alloimmun...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000000867
更新日期:2016-03-01 00:00:00
abstract::Two infants born to mothers who had been given a renal transplant and who were receiving azathioprine and prednisone have been followed up for over 2 years. Initially both had lower than normal lymphocyte counts and cortisol levels. Chromosomal aberrations similar to those found in the lymphocytes of the mothers were ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197604000-00004
更新日期:1976-04-01 00:00:00