Effect of subacute benzene exposure on the activity of two neuropeptide-degrading enzymes in the rat brain.

Abstract:

:Benzene (Bz) is an important industrial chemical, a petroleum by-product, a component of unleaded gas, and thus a ubiquitous environmental pollutant. It is well established that this organic solvent possesses neurotoxic and behavioral effects. However, the neurochemical mechanism of the solvent action remains obscure. The aminopeptidases (AP) are proteolytic enzymes that have been proposed as a candidate regulator of the degradation of several neuropeptides. In this work, changes in Lys- and Leu-aminopeptidase activities in several rat brain regions after benzene administration are described. The AP activity was determined by measuring the rate of hydrolysis of the artificial substrates Lys- and Leu-2-naphthylamides (fluorimetrically detected in triplicate). Both enzyme activities decrease in the thalamus, hypothalamus, hippocampus, and amygdala after Bz treatment. It is suggested that these aminopeptidase activities play a part in the benzene action mechanism, possibly by regulating the activity of several neuroactive peptides.

authors

de Gandarias JM,Casis O,Irazusta J,Echevarría E,Casis L

doi

10.1007/BF00212097

subject

Has Abstract

pub_date

1992-04-01 00:00:00

pages

345-8

issue

3

eissn

0090-4341

issn

1432-0703

journal_volume

22

pub_type

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