Abstract:
PURPOSE:Heparin immobilized porous poly(D,L-lactic-co-glycolic acid) (PLGA) microspheres were prepared for sustained release of basic fibroblast growth factor (bFGF) to induce angiogenesis. MATERIALS AND METHODS:Porous PLGA microspheres having primary amine groups on the surface were prepared using an oil-in-water (O/W) single emulsion method using Pluronic F-127 as an extractable porogen. Heparin was surface immobilized via covalent conjugation. bFGF was loaded into the heparin functionalized (PLGA-heparin) microspheres by a simple dipping method. The bFGF loaded PLGA-heparin microspheres were tested for in vitro release and in vivo angiogenic activity. RESULTS:PLGA microspheres with an open-porous structure were formed. The amount of conjugated amine group onto the microspheres was 1.93+/-0.01 nmol/mg-microspheres, while the amount of heparin was 95.8 pmol/mg-microspheres. PLGA-heparin microspheres released out bFGF in a more sustained manner with a smaller extent of initial burst than PLGA microspheres, indicating that surface immobilized heparin controlled the release rate of bFGF. Subcutaneous implantation of bFGF loaded PLGA-heparin microspheres in mice significantly induced the formation of new vascular microvessels. CONCLUSIONS:PLGA microspheres with an open porous structure allowed significant amount of heparin immobilization and bFGF loading. bFGF loaded PLGA-HP microspheres showed sustained release profiles of bFGF in vitro, demonstrating reversible and specific binding of bFGF to immobilized heparin. They also induced local angiogenesis in vivo in an animal model.
journal_name
Pharm Resjournal_title
Pharmaceutical researchauthors
Chung HJ,Kim HK,Yoon JJ,Park TGdoi
10.1007/s11095-006-9039-9subject
Has Abstractpub_date
2006-08-01 00:00:00pages
1835-41issue
8eissn
0724-8741issn
1573-904Xjournal_volume
23pub_type
杂志文章abstract::A method is described for the assay of phenobarbital N-glucosylation using UDP-D-[6-3H]glucose. The radioactive phenobarbital N-glucoside conjugates [(5R)-PBG, (5S)-PBG] formed during the incubations were resolved from each other and from uncharacterized radioactive products by semipreparative HPLC. The product ratio ...
journal_title:Pharmaceutical research
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journal_title:Pharmaceutical research
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journal_title:Pharmaceutical research
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更新日期:2008-03-01 00:00:00
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更新日期:2010-09-01 00:00:00
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pub_type: 临床试验,杂志文章
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journal_title:Pharmaceutical research
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更新日期:2004-09-01 00:00:00
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journal_title:Pharmaceutical research
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更新日期:2001-06-01 00:00:00
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更新日期:2015-02-01 00:00:00
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journal_title:Pharmaceutical research
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更新日期:2020-07-13 00:00:00
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journal_title:Pharmaceutical research
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更新日期:1993-08-01 00:00:00
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journal_title:Pharmaceutical research
pub_type: 杂志文章
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更新日期:2001-03-01 00:00:00
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journal_title:Pharmaceutical research
pub_type: 杂志文章
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更新日期:1995-06-01 00:00:00
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journal_title:Pharmaceutical research
pub_type: 杂志文章
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更新日期:2006-03-01 00:00:00