Abstract:
:Isolated liver from phenobarbital-induced male mice was perfused using infusions of cytochrome c pulses as quality control of the system. Livers spontaneously evolved 1.1 pmoles ethane g-1 liver min-1, exogenous pentane disappeared with 0.6 pmoles g-1 min-1. Infusion of 0.26 mmoles/l. FeCl2 led to immediate ethane production followed later on by lactate dehydrogenase release from the liver. Infusion of acetaminophen resulted in hepatic ethane production at drug concentrations greater than 0.1 mmoles/l. A maximum effect was observed at 2 mmoles/l. of acetaminophen infused while higher concentrations, up to 10 mmoles/l. delayed ethane release although they enhanced the rate of glutathione depletion. This glutathione efflux decreased from 12 nmoles/g-1 min-1 observed after perfusion of medium alone to 2.9 nmoles/g-1 min-1 when AAP was infused. The slope of the pseudo first order depletion kinetics depended on the acetaminophen concentrations. This glutathione release represents the perisinusoidal portion of the total efflux measured here independently from the biliary secretion. The results show that in agreement with the in vivo findings acute intoxication of liver with high doses of this drug lead to lipid peroxidation, while in vitro an apparent antioxidative effect was measured. The implications for drug screening are probably important.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Thelen M,Wendel Adoi
10.1016/0006-2952(83)90112-0subject
Has Abstractpub_date
1983-06-01 00:00:00pages
1701-6issue
11eissn
0006-2952issn
1873-2968pii
0006-2952(83)90112-0journal_volume
32pub_type
杂志文章abstract::The cytotoxicity anti-tumour intercalating agents such as the anthraquinone mitoxantrone is thought to relate to DNA binding and the trapping of DNA topoisomerase II complexes on cellular DNA. We have studied the uptake, nuclear location, DNA binding mode and DNA damaging capacity of mitoxantrone in a small cell lung ...
journal_title:Biochemical pharmacology
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更新日期:1987-12-15 00:00:00
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更新日期:2005-06-15 00:00:00
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journal_title:Biochemical pharmacology
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更新日期:1983-05-15 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1983-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-03-01 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
doi:10.1016/s0006-2952(99)00008-8
更新日期:1999-05-01 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:1994-03-15 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:1990-06-15 00:00:00