Abstract:
:Incubation of cultured embryonic chicken heart cells with transforming growth factor beta 1 (TGF-beta 1) resulted in a concentration- and time-dependent decrease in the number of muscarinic acetylcholine receptors (mAChR), which reached a maximum by 24 hr. Twenty-four hours following TGF-beta 1 treatment, cm2 and cm4 mAChR protein levels were decreased 59 and 41%, respectively, and cm2 mRNA and cm4 mRNA levels were both decreased by approximately 40%. Chick heart cells treated with TGF-beta 1 exhibited a decreased sensitivity for carbachol-mediated inhibition of adenylyl cyclase activity compared with control cells. Thus, TGF-beta 1 stimulation of chick heart cells results in decreases in the expression of both cm2 and cm4 mAChR and in muscarinic responsiveness.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Jackson DA,Nathanson NMdoi
10.1016/s0006-2952(97)00219-0subject
Has Abstractpub_date
1997-08-15 00:00:00pages
525-7issue
4eissn
0006-2952issn
1873-2968pii
S0006-2952(97)00219-0journal_volume
54pub_type
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journal_title:Biochemical pharmacology
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:1995-03-01 00:00:00