Abstract:
:RIAs specific for the alpha-subunit of glycoprotein hormones were used to monitor the subunit conformation in ovine FSH and TSH and in recombinants of ovine LH alpha with (ovine and porcine) LH beta and hCG beta. Differences in log-logit slopes of the RIA dilution curves were interpreted to indicate changes, presumably conformational, in the local environment of the antigenic determinants of the alpha-subunit. In all but one case, free ovine LH alpha yielded a slope that was distinct from those of the hormones and recombinants. FSH, TSH, and the homologous LH recombinant all exhibited different slopes, and the recombinant ovine LH alpha-hCG beta was characterized by a slope that was distinct from the identical slopes of the recombinants ovine LH alpha-ovine LH beta and ovine LH alpha-porcine LH beta. These results suggest that a conformational change occurs in the alpha-subunit upon association with a beta-subunit and that different beta-subunits may induce distinct conformations in a common alpha-subunit.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Strickland TW,Puett Ddoi
10.1210/endo-111-1-95subject
Has Abstractpub_date
1982-07-01 00:00:00pages
95-100issue
1eissn
0013-7227issn
1945-7170journal_volume
111pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::The pancreatic polypeptide cell, the only mature endocrine cell in the fetal pig pancreas, produces equimolar amounts of two peptides, pancreatic polypeptide and pancreatic icosapeptide, from the same precursor. The amino acid sequence of pancreatic polypeptide is more homogeneous among species, whereas pancreatic ico...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.10.8409
更新日期:2001-10-01 00:00:00
abstract::GnRH, the main regulator of reproduction, is produced in a variety of tissues outside of the hypothalamus, its main site of synthesis and release. We aimed to determine whether GnRH produced in the female rat pituitary and ovaries is involved in the processes leading to ovulation. We studied the expression patterns of...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0240
更新日期:2005-08-01 00:00:00
abstract::Whereas the stimulatory effect of interleukin-6 (IL-6) on the hypothalamic-pituitary-adrenal (HPA) axis is well established, its mode of action in this axis has yet to be fully elucidated. To further study the role of IL-6 in the HPA axis, we compared the expression of IL-6 messenger RNA (mRNA) in the rat hypothalamus...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.6.8243280
更新日期:1993-12-01 00:00:00
abstract::The effect of osmotic changes on aldosterone production, [Ca2+]i and voltage-gated Ca2+ currents, was studied in cultured rat glomerulosa cells. Alteration of osmolarity by sucrose addition in the 250-330 mosM range did not influence aldosterone production per se, but it substantially affected K+-stimulated aldosteron...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.5.7465
更新日期:2000-05-01 00:00:00
abstract::We have measured the concentrations of vasopressin in sequential samples of cerebrospinal fluid (CSF) obtained from fetal lambs between 108 and 130 days gestation. There was a clear and significant (F = 4.46; P less than 0.005) rhythm in vasopressin concentrations in the CSF of nine fetuses, characterized by a mean tr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-6-3095
更新日期:1989-06-01 00:00:00
abstract::The response of purified rat testicular peritubular myoid cells (PMC) to platelet-derived growth factor (PDGF) was studied. Freshly isolated PMC were devoid of measurable amounts of PDGF-binding sites. However, after 1 day in culture in serum-free conditions, specific high affinity receptors were detected. The estimat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.4.8404630
更新日期:1993-10-01 00:00:00
abstract::Current drug therapies for obesity are ineffective, and existing treatments for lipid disorders can be further improved. Thyroid hormones affect both conditions, although currently available nonselective thyromimetics are not clinically useful for such treatment due to cardiac side effects. Recent studies suggest that...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0973
更新日期:2004-04-01 00:00:00
abstract::Amplification of insulin secretion by cAMP is mediated by protein kinase A (PKA) and exchange protein directly activated by cAMP (Epac). Using selective activators, we determined how each effector influences the cytosolic free Ca(2+) concentration ([Ca(2+)]c) and insulin secretion in mouse islets. Alone PKA activator ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1247
更新日期:2014-09-01 00:00:00
abstract::Prolonged stimulation of cells by beta-adrenergic receptor agonists may lead to diminished responsiveness of the cells to subsequent activation by catecholamines. This phenomenon has been termed desensitization; the mechanism(s) for desensitization may involve an apparent loss in the number of beta-adrenergic receptor...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-4-1272
更新日期:1984-04-01 00:00:00
abstract::Maternal obesity at conception increases the risk of offspring obesity, thus propagating an intergenerational vicious cycle. Male offspring born to obese dams are hyperresponsive to high fat-diets, gaining greater body weight, fat mass, and additional metabolic sequelae compared to lean controls. In this report, we id...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1078
更新日期:2011-11-01 00:00:00
abstract::Female and male brains differ. Differences begin early during development due to a combination of genetic and hormonal events and continue throughout the lifespan of an individual. Although researchers from a myriad of disciplines are beginning to appreciate the importance of considering sex differences in the design ...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2004-1142
更新日期:2005-04-01 00:00:00
abstract::Previous studies had suggested that norepinephrine (NE) and its precursors dopamine (DA) and L-DOPA acted similarly on iodine metabolism of isolated thyroid cells. Present studies indicate that this similarity extends to the inhibition by catecholamines of TSH-stimulated T4 release by mouse thyroids incubated in vitro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-2-632
更新日期:1986-02-01 00:00:00
abstract::The health impacts of endocrine-disrupting chemicals (EDCs) remain debated, and their tissue and molecular targets are poorly understood. In this study, we leveraged systems biology approaches to assess the target tissues, molecular pathways, and gene regulatory networks associated with prenatal exposure to the model ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00817
更新日期:2019-02-01 00:00:00
abstract::Biological actions of natriuretic peptide (NP) are determined by the condition of the receptor as well as that of the hormone. Although we previously demonstrated in hypertensive rats the up-regulation of NP-A receptor that mediates various biological actions of NPs, the pathophysiologic significance of NP-C receptor,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.3.8603580
更新日期:1996-03-01 00:00:00
abstract::The inhibitory effect of T3 on TSH release was studied on a population of thyrotroph-enriched cells prepared from bovine pituitary glands by centrifugal elutriation. The cells (2.0 X 10(5)/ml) were cultured for 2 days and then exposed to TRH, phorbol-12 myristate-13 acetate (PMA), or calcium ionophore (A23187) with or...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-5-2121
更新日期:1988-05-01 00:00:00
abstract::Two corticotropin-releasing factor 2 receptor (CRF2R)-selective peptides have been recently described, urocortin II (also known as stresscopin-related peptide) and urocortin III (stresscopin). We have used urocortin II to evaluate the effects of activation of the CRF2R on skeletal muscle-related physiological processe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0271
更新日期:2003-11-01 00:00:00
abstract::Bovine placental lactogen (bPL) was found to be as potent as human GH (hGH) in its ability to bind to soluble full-size recombinant hGH-binding protein (hGHBP) and to membrane-embedded hGH receptor in intact IM-9 human lymphocytes. bPL was also capable of forming a 1:2 complex with hGHBP, although the structure of thi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.3.7867580
更新日期:1995-03-01 00:00:00
abstract::Insulin-like growth factors (IGFs), when isolated from serum or tissue fluids, are usually found as part of a protein complex which also contains one of several IGF binding proteins (IGFBPs). Although some IGFBPs have been shown to alter interactions of IGFs with their receptors in vitro and can modify the responses o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.2.7507840
更新日期:1994-02-01 00:00:00
abstract::Impaired trophoblast invasion is associated with pregnancy disorders such as early pregnancy loss and preeclampsia. There is evidence to suggest that the consumption of caffeine during pregnancy may increase the risk of pregnancy loss; however, little is known about the direct effect of caffeine on normal trophoblast ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1930
更新日期:2012-09-01 00:00:00
abstract::The effects of morphine on the release of dopamine from tuberoinfundibular neurons and the release of PRL from the anterior pituitary gland were studied in diestrous female rats. Morphine ions ejected iontophoretically in minute quantities into the arcuate nuclei (using one electrode per nucleus) were found to reduce ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-3-767
更新日期:1981-03-01 00:00:00
abstract::Specific serum binding of 25-hydroxy-cholecalciferol (25-OHD3) was measured by saturation analysis in rats of various ages, and during vitamin D deprivation. The serum binding capacity for 25-OHD3 was observed to increase until age 6-8 weeks, then decline and remain stable thereafter at 3.7 x 10(-6) M. The 25-hydroxyv...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-3-642
更新日期:1977-03-01 00:00:00
abstract::The rat uterine enzyme glycerylphosphorylcholine (GPC) diesterase found in the proestrous secretions was purified and characterized biochemically with respect to its subunit mol wt, native size, pI, and amino acid and carbohydrate composition. The 30-kDa protein was assessed to have a native mol wt of 105 kDa, as dete...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-3-1147
更新日期:1991-09-01 00:00:00
abstract::The genetically obese Zucker rat is a widely used model of early-onset obesity. Like obese children, these obese rats are hyperinsulinemic and have low GH secretion. However, data on linear growth and insulin-like growth factor-I (IGF-I) levels in this model are scanty and contradictory. In the present study, we inves...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.3.7509740
更新日期:1994-03-01 00:00:00
abstract::The glucocorticoid (GC) rhythm is entrained to light-dark (LD) cycles via a molecular clock in the suprachiasmatic nucleus (SCN) and is maintained by an adrenal clock synchronized by SCN-dependent signals. Targeted deletion of the core clock gene Bmal1 can disrupt adrenal clock function. The requirement of the adrenal...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00769
更新日期:2018-12-01 00:00:00
abstract::Caveolae and their principal component caveolin have been implicated in playing a major role in G protein-mediated transmembrane signaling. We examined whether caveolin interacts with adenylyl cyclase, an effector of G protein signaling, using a 20-mer peptide derived from the N-terminus scaffolding domain of caveolin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.4.5957
更新日期:1998-04-01 00:00:00
abstract::The effects of undernutrition on the timing of puberty and regulation of LH secretion by the inhibitory and stimulatory feedback action of estradiol were examined in female sheep. The first experiment determined that maintenance of low BW (ca., 20 kg, four lambs) between 10 and 45 weeks of age prevented initiation of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-1-375
更新日期:1985-01-01 00:00:00
abstract::The conceptus and endometrium secrete large amounts of prostaglandin E₂ (PGE₂) into the porcine uterine lumen during the periimplantation period. We hypothesized that PGE₂ acts on conceptus/trophoblast cells through auto- and paracrine mechanisms. Real-time RT-PCR analysis revealed that PGE₂ receptor (PTGER)2 mRNA was...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-2271
更新日期:2013-10-01 00:00:00
abstract::We have reported that calcitonin (CT) treatment induced downregulation of the CT receptor (CTR) in mouse osteoclast-like cells (OCLs). Here, we studied the features of homologous down-regulation of the CTR in mature mouse OCLs. Treatment with salmon CT (sCT) and human CT (hCT) reduced [125I]sCT specific binding. The d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.1.8536630
更新日期:1996-01-01 00:00:00
abstract::Local androgen synthesis in prostate cancer (PC) may contribute to the development of castration-resistant PC (CRPC), but pathways controlling intratumoral steroidogenic enzyme expression in PC are unknown. We investigated the effects of activin, a factor involved in the regulation of PC growth and steroidogenic enzym...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-2065
更新日期:2012-12-01 00:00:00
abstract::SH2-containing inositol 5'-phosphatase 2 (SHIP2) is a 5'-lipid phosphatase hydrolyzing the phosphatidylinositol (PI) 3-kinase product PI(3,4,5)P(3) to PI(3,4)P(2) in the regulation of insulin signaling, and is shown to be increased in peripheral tissues of diabetic C57BL/KSJ-db/db mice. To clarify the impact of SHIP2 ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0820
更新日期:2008-02-01 00:00:00