Abstract:
:Na+/Ca2+-K+ exchangers (NCKX) are plasma membrane transporters that are thought to mainly mediate Ca2+ extrusion (along with K+) at the expense of the Na+ electrochemical gradient. However, because they are bidirectional, most assays have relied on measuring their activity in the reverse (Ca2+ import) mode. Herein we describe a method to control intracellular ionic conditions, and examine the forward (Ca2+ extrusion) mode of exchange of NCKX2.
journal_name
Ann N Y Acad Scijournal_title
Annals of the New York Academy of Sciencesauthors
Altimimi HF,Schnetkamp PPdoi
10.1196/annals.1387.056subject
Has Abstractpub_date
2007-03-01 00:00:00pages
29-33eissn
0077-8923issn
1749-6632pii
annals.1387.056journal_volume
1099pub_type
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journal_title:Annals of the New York Academy of Sciences
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