Abstract：
:Identification of lipophilic chemicals requires the development of new techniques to detect these compounds at the cellular and subcellular levels. To address this issue, we have developed a combinational protocol using antibodies directed against chemicals together with a multiple signal amplification system (catalyzed signal amplification/gold-substituted silver-intensified peroxidase) to detect the lipophilic compounds at their subcellular sites of accumulation by transmission electron microscopy. As a model organism, we exposed the blue mussel Mytilus edulis to the polycyclic aromatic hydrocarbon phenanthrene and the polychlorinated biphenyl mixture Aroclor 1254 for up to 10 d. The aim of these exposure experiments was to analyze the cellular targets and sites of accumulation in the digestive system by transmission electron microscopy. The endo-lysosomal system of digestive cells and mitochondria of epithelial cells appeared to be the preferred accumulation sites for the lipophilic compounds studied. The antibody-based approach to detect lipophilic chemicals provides an important insight into the pathways of uptake and accumulation as well as mechanisms of toxicity.
journal_name
Environ Toxicol Chemjournal_title
Environmental toxicology and chemistryauthors
Einsporn S,Koehler Adoi
10.1897/07-348.1subject
Has Abstractpub_date
2008-03-01 00:00:00pages
554-60issue
3eissn
0730-7268issn
1552-8618pii
07-348journal_volume
27pub_type
杂志文章abstract：:The present study aims at investigating the effects of Zn, Ca, and dissolved organic carbon (DOC) on the waterborne Cd bioaccumulation of a freshwater bivalve (Dreissena polymorpha). Mussels were exposed for 48 h at 3 µg/L of Cd in different media. Their physiological activities were assessed by separately measuring t...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1002/etc.287
更新日期：2010-10-01 00:00:00
abstract：:Wetlands are important habitats and spawning grounds to a diverse wildlife population and are particularly vulnerable to adverse effects of endocrine disruptors. Many pesticides possess hormonal activity and have thus been classified as endocrine disruptors. A new analytical method based on ultrasonic-assisted extract...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1897/07-511.1
更新日期：2008-05-01 00:00:00
abstract：:Ten agronomic plant species were exposed to different concentrations of nano-titanium dioxide (nTiO2 ) or nano-cerium oxide (nCeO2 ) (0 μg/mL, 250 μg/mL, 500 μg/mL, and 1000 μg/mL) to examine potential effects on germination and early seedling development. The authors modified a standard test protocol developed for so...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1002/etc.3374
更新日期：2016-09-01 00:00:00
abstract：:The assessment of chemicals as bioaccumulative in the regulatory process makes use of the bioconcentration factor as a metric. However, this metric does not account for the dietary uptake route and therefore cannot be applied to terrestrial food chains. In recent years, the biomagnification factor (BMF) and the trophi...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1002/etc.2229
更新日期：2013-07-01 00:00:00
abstract：:As contaminant exposures in aquatic ecosystems continue to increase, the need for streamlining research efforts in environmental toxicology using predictive frameworks also grows. One such framework is the adverse outcome pathway (AOP). An AOP framework organizes and utilizes toxicological information to connect measu...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1002/etc.4757
更新日期：2020-08-01 00:00:00
abstract：:Microcystis aeruginosa is a dominant cyanobacterium commonly found in Chinese freshwater ecosystems during phytoplankton blooms, and copper sulfate is one of the most frequently used algicides for controlling the nuisance blooms. In this study, we examined the effects of varied water chemistry (dissolved organic matte...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1002/etc.266
更新日期：2010-10-01 00:00:00
abstract：:The uptake of Se by the freshwater alga Chlamydomonas reinhardtii and the subsequent transfer to the Asiatic clam Corbicula fluminea was investigated. The objective was to investigate the bioavailability of algal-bound Se for C. fluminea while taking into account Se speciation and bivalve ventilation. First, uptake ra...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1897/05-386r1.1
更新日期：2006-10-01 00:00:00
abstract：:Blood samples from lizards (Gallotia galloti) collected from two agricultural areas (Las Galletas and Punta del Hidalgo) and two reference areas on the Island of Tenerife (Canary Islands, Spain) were analyzed for butyrylcholinesterase (BChE) activity. Serum BChE activity was characterized first by in vitro experiments...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：
更新日期：2003-02-01 00:00:00
abstract：:Soils and sediments in the floodplain of the Tittabawassee River downstream of Midland, Michigan, USA contain elevated concentrations of polychlorinated dibenzofurans (PCDF) and polychlorinated dibenzo-p-dioxins (PCDD). As a long-lived, resident top predator, the great horned owl (Bubo virginianus; GHO) has the potent...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1002/etc.291
更新日期：2010-10-01 00:00:00
abstract：:The present study evaluated Se bioaccumulation in four waterbird species (n=206 birds) that breed within San Francisco Bay, California, U.S.A.: American avocets (Recurvirostra americana), black-necked stilts (Himantopus mexicanus), Forster's terns (Sterna forsteri), and Caspian terns (Hydroprogne caspia). Selenium con...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1897/09-101.1
更新日期：2009-10-01 00:00:00
abstract：:We analyzed the fate of organotins in seawater and sediments from Tokyo Bay, Japan, by gas chromatography/inductively coupled plasma mass spectrometry. We also measured the toxicity of methylated butyltins by in vitro bioassays, the retinoid X receptor (RXR) activation method, and the marine luminescent bacterium Vibr...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1897/06-599.1
更新日期：2007-12-01 00:00:00
abstract：:The rapid development of new technologies such as transcriptomics, proteomics, and metabolomics (Omics) are changing the way ecotoxicology is practiced. The data deluge has begun with genomes of over 65 different aquatic species that are currently being sequenced, and many times that number with at least some level of...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章,评审
doi：10.1002/etc.401
更新日期：2011-02-01 00:00:00
abstract：:The anion perchlorate (ClO4-) is an oxidizing component commonly used in solid propellants for rockets and missiles; in explosives, flares, fireworks, chemical processes, and automobile air-bag inflators; and for other assorted uses. With recent advances in analytical detection capability, perchlorate has been found i...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章,评审
doi：10.1897/02-648
更新日期：2004-06-01 00:00:00
abstract：:Adsorption of organic compounds onto hard carbon constituents of soils and sediments may be described by a dual Langmuir-like equation for adsorption onto high-energy sites and low-energy sites. To describe quantitatively the sorbate-sorbent interactions on these high-energy sites and low-energy sites, Gibbs free ener...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1897/06-218r.1
更新日期：2006-12-01 00:00:00
abstract：:Firemaster 550 and Firemaster BZ-54 are two brominated formulations that are in use as replacements for polybrominated diphenyl ether (PBDE) flame retardants. Two major components of these mixtures are 2,3,4,5-tetrabromo-ethylhexylbenzoate (TBB) and 2,3,4,5-tetrabromo-bis(2-ethylhexyl) phthalate (TBPH). Both have been...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1002/etc.94
更新日期：2010-03-01 00:00:00
abstract：:The present study examined the relative importance of copper (aqueous Cu and CuO particles of different sizes) added to sediment to determine the bioaccumulation, toxicokinetics, and effects in the deposit feeder Potamopyrgus antipodarum. In experiment 1, the bioaccumulation of Cu (240 µg Cu/g dry wt of sediment) adde...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1002/etc.2216
更新日期：2013-07-01 00:00:00
abstract：:The paucity of information on the occurrence of pharmaceuticals in the environment in African countries led the authors to investigate 8 acidic pharmaceuticals (4 antipyretics, 3 antibiotics, and 1 lipid regulator) in wastewater, surface water, and sediments from the Msunduzi River in the province of KwaZulu-Natal, So...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1002/etc.3144
更新日期：2016-01-01 00:00:00
abstract：:Based on weight loss in water, 24 h is recommended for Tubifex tubifex gut clearance. Biota-to-sediment accumulation factors (BSAFs) in gut-cleared T. tubifex following six weeks of exposure to Cd-, Ni-, and Pb-spiked sediment were 12.4, 3.0, and 19.0, respectively. Tissue Ni concentrations peaked after 12 h, whereas ...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1897/02-415
更新日期：2004-01-01 00:00:00
abstract：:The present study investigated the impact of nano titanium dioxide (nTiO(2) ) exposure on the cellular structures of the nitrogen-fixing cyanobacteria Anabaena variabilis. Results of the present study showed that nTiO(2) exposure led to observable alteration in various intracellular structures and induced a series of ...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1002/etc.445
更新日期：2011-04-01 00:00:00
abstract：:The present study determined the effects of environmentally relevant, short-term (4-h) exposure to the pyrethroid insecticide esfenvalerate on mortality, food consumption, growth, swimming ability, and predation risk in larvae of the fathead minnow (Pimephales promelas). Acute effect concentrations were determined, an...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1897/07-448.1
更新日期：2008-08-01 00:00:00
abstract：:This study evaluated the interactions of flight, fasting, and 1,1,1-trichloro-bis(4-chlorophenyl)ethane (p,p'-DDT) loading on residue metabolism and distribution in recently exposed white-crowned sparrows (Zonotrichia leucophrys). Female sparrows were dosed with 5 mg p,p'-DDT per kg body weight over 3 d. Following 1 d...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1002/etc.730
更新日期：2012-02-01 00:00:00
abstract：:Until recently, the behaviors of antibiotics and their ecotoxicological impact have been overlooked in the environment. The topic is broad and encompasses a wide range of organisms including microorganisms, algae, invertebrates, and vertebrates inhabiting various aquatic ecosystems. Changing the equilibrium of any 1 c...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章,评审
doi：10.1002/etc.3552
更新日期：2016-11-01 00:00:00
abstract：:The reference method for the Canada-wide standard (CWS) for petroleum hydrocarbons (PHCs) in soil provides laboratories with methods for generating accurate and reproducible soil analysis results. The CWS PHC tier 1 generic soil-quality guidelines apply to 4 carbon ranges/fractions: F1 (C6-C10), F2 (C10-C16), F3 (C16-...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1002/etc.2578
更新日期：2014-08-01 00:00:00
abstract：:Prior to the implementation of any new technology, possible environmental and health repercussions first must be researched. Fullerenes are to be produced soon on an industrial scale, with applications quickly following. To investigate the possible environmental impact of fullerenes, a C60-water suspension (nano-C60) ...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1897/04-649r.1
更新日期：2005-11-01 00:00:00
abstract：:Per- and polyfluoroalkyl substances (PFAS) often occur together as contamination in exposure media such as drinking water or food. The relative potency factor (RPF) methodology facilitates the risk assessment of mixture exposure. A database of liver endpoints was established for 16 PFAS, using data with the same speci...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1002/etc.4835
更新日期：2020-07-30 00:00:00
abstract：:Rice is the main crop in the subbasin of the fluvial lagoon system of Palizada River (FLSPR) in the state of Campeche, Mexico. The pesticides used to control pests of this crop mainly are carbofuran, chlorpyrifos, and glyphosate. Black-bellied whistling duck (Dendrocygna autumnalis) is an ecologically and economically...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1897/03-646.1
更新日期：2005-02-01 00:00:00
abstract：:Toxicity tests in invertebrates often use sublethal endpoints, which may exhibit different sensitivity for various toxicants. Our objective was to characterize the sensitivity of movement, feeding, growth, and reproduction as endpoints for heavy metal toxicity testing with Caenorhabditis elegans. Growth and feeding we...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：
更新日期：2001-04-01 00:00:00
abstract：:The rising potential for the release of engineered nanoparticles (ENPs) into aquatic environments requires evaluation of risks to protect ecological health. The present review examines knowledge pertaining to the interactions of metal-based ENPs with aquatic higher plants, identifies information gaps, and raises consi...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章,评审
doi：10.1002/etc.3364
更新日期：2016-07-01 00:00:00
abstract：:Silencing genes of a pest with double-stranded RNA (dsRNA) is a promising new pest management technology. As part of the environmental risk assessment for dsRNA-based products, the environmental fate and the potential for adverse effects to on-target organisms should be characterized. In the present study, a nonbioact...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1002/etc.3648
更新日期：2017-05-01 00:00:00
abstract：:The toxicities of chemical mixtures containing 10 compounds, detected in wastewater treatment plant (WWTP) effluents, were investigated using Daphnia magna in a two-step prediction (TSP) model. The 10 chemicals determined by gas chromatography/ mass spectrometry in WWTP effluents included three groups: Three acetylcho...
journal_title：Environmental toxicology and chemistry
pub_type： 杂志文章
doi：10.1897/05-484r.1
更新日期：2006-08-01 00:00:00
