Abstract:
:We characterize the dark-adapted photoresponses from mouse cones intact in the isolated retina, their virtually natural environment, by isolating pharmacologically the photoreceptor light responses from the electroretinogram (ERG). Due to the different photoresponse kinetics and sensitivity of rods and cones, the cone responses were readily attained by using a rod-saturating preflash. The stimulus wavelength (544 nm) was chosen to selectively stimulate the green sensitive ("M"-)pigment. Obtained responses were monophasic, showing fast kinetics (mean t(p)=51 ms) and low sensitivity (fractional single-photon response ca. 0.23%). The amplification coefficient of cones (4.6 s(-2)) was very close to that of rods (5.6 s(-2)), while the dominant time constant of recovery was clearly smaller for cones (33 ms) than for rods (160 ms).
journal_name
Vision Resjournal_title
Vision researchauthors
Heikkinen H,Nymark S,Koskelainen Adoi
10.1016/j.visres.2007.11.005subject
Has Abstractpub_date
2008-01-01 00:00:00pages
264-72issue
2eissn
0042-6989issn
1878-5646pii
S0042-6989(07)00507-Xjournal_volume
48pub_type
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