Abstract:
:The present study evaluated the effects of field metal contamination on sperm motility and the RNA/DNA ratio in echinoderms. Populations of Asterias rubens and Echinus acutus that occur naturally along a contamination gradient of sediments by cadmium, copper, lead, and zinc in a Norwegian fjord (the Sørfjord) were studied. Sperm motility, a measure of sperm quality, was quantified using a computer-assisted sperm analysis system. The RNA/DNA ratio, a measure of protein synthesis, was assessed by a one-dye (ethidium bromide)/one-enzyme (RNase), 96-well microplate fluorometric assay. Although both species accumulate metals at high concentrations, neither sperm motility parameters in A. rubens nor the RNA/DNA ratio in both species were affected. The Sørfjord is still one of the most metal-contaminated marine sites in Europe, but even so, populations of A. rubens and E. acutus are able to endure under these conditions.
journal_name
Environ Toxicol Chemjournal_title
Environmental toxicology and chemistryauthors
Catarino AI,Cabral HN,Peeters K,Pernet P,Punjabi U,Dubois Pdoi
10.1897/07-402subject
Has Abstractpub_date
2008-07-01 00:00:00pages
1553-60issue
7eissn
0730-7268issn
1552-8618pii
07-402journal_volume
27pub_type
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