Challenges in current adult fish laboratory reproductive tests: suggestions for refinement using a mummichog (Fundulus heteroclitus) case study.

Abstract:

:Concerns about screening endocrine-active contaminants have led to the development of a number of short-term fish reproductive tests. A review conducted of 62 published adult fish reproductive papers using various fish species found low samples sizes (mean of 5.7 replicates with a median of 5 replicates) and high variance (an average coefficient of variance of 43.8%). The high variances and low sample sizes allow only relatively large differences to be detected with the current protocols; the average significant difference detected was a 68.7% reduction in egg production, while only differences above 50% were detected with confidence. This result indicates low power to detect more subtle differences and a high probability of type II errors in interpretation. The present study identifies several ways to increase the power of the adult fish reproductive test in the mummichog (Fundulus heteroclitus). By identifying the peak timing of egg production (before and after the new moon), extending the duration of the experiment (increased from 7 to 14 d), and determining that a sample size of eight replicate tanks per treatment accurately predicts variance in the sample population (based on pre-exposure variation calculations of replicate tanks), the power of the test has been significantly increased. The present study demonstrates that weaknesses in the current adult fish reproductive tests can easily be addressed by focusing on improved understanding of the reproductive behavior of the test species and developing study designs that include calculating desired variability levels and increasing replicates.

journal_name

Environ Toxicol Chem

authors

Bosker T,Munkittrick KR,MacLatchy DL

doi

10.1897/09-032.1

subject

Has Abstract

pub_date

2009-11-01 00:00:00

pages

2386-96

issue

11

eissn

0730-7268

issn

1552-8618

pii

09-032

journal_volume

28

pub_type

杂志文章
  • Cumulative impacts assessment along a large river, using brown bullhead catfish (Ameiurus nebulosus) populations.

    abstract::The effects of point-source and diffuse discharges on resident populations of brown bullhead catfish (Ameiurus nebulosus (LeSueur, 1819)) in the Waikato River (New Zealand) were assessed at sites both upstream and downstream of point-source discharges. At each site, the population parameters, relative abundance, age s...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/05-315r.1

    authors: West DW,Ling N,Hicks BJ,Tremblay LA,Kim ND,Van den Heuvel MR

    更新日期:2006-07-01 00:00:00

  • Comparing median lethal concentration values using confidence interval overlap or ratio tests.

    abstract::Experimenters in toxicology often compare the concentration-response relationship between two distinct populations using the median lethal concentration (LC50). This comparison is sometimes done by calculating the 95% confidence interval for the LC50 for each population, concluding that no significant difference exist...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/05-320r.1

    authors: Wheeler MW,Park RM,Bailer AJ

    更新日期:2006-05-01 00:00:00

  • Modeling the toxicity of polar and nonpolar narcotic compounds to luminescent bacterium Shk1.

    abstract::Luminescent bacterium Shk1 was created for the purpose of testing and screening the toxicity of activated sludge wastewater treatment plant influent to avoid toxic shock to the wastewater treatment plant microorganisms. The toxicity of a number of organic compounds was tested using an assay employing Shk1. Because the...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:

    authors: Ren S,Frymier PD

    更新日期:2002-12-01 00:00:00

  • Effects of Non-native Fish on Lacustrine Food Web Structure and Mercury Biomagnification along a Dissolved Organic Carbon Gradient.

    abstract::Although the introduction of non-native fish species has been shown to alter trophic ecology in aquatic ecosystems, there has been limited research on how invasive species alter methylmercury (MeHg) biomagnification in lacustrine food webs. We sampled surface water and biota from 8 lakes in Quebec, Canada, spanning a ...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.4831

    authors: Barst BD,Hudelson K,Lescord GL,Santa-Rios A,Basu N,Crémazy A,Drevnick PE

    更新日期:2020-11-01 00:00:00

  • Complex mixture toxicity for single and multiple species: proposed methodologies.

    abstract::Methods for the assessment of ecological risks associated with exposure to defined mixtures of toxicants are reviewed and formalized for single-species toxicity. Depending on the modes of action of toxicants in a mixture, these methods apply either the model for concentration additivity (CA) or the model for response ...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章,评审

    doi:10.1897/04-639r.1

    authors: De Zwart D,Posthuma L

    更新日期:2005-10-01 00:00:00

  • Determination of in situ polychlorinated biphenyl elimination rate coefficients in the freshwater mussel biomonitor Elliptio complanata deployed in the Huron-Erie corridor, southeast Michigan, USA, and southwest Ontario, Canada.

    abstract::Quantitative mussel biomonitoring studies often use laboratory-measured whole-body elimination rate coefficients (k(tot) in conjunction with concentration data from field-deployed mussels to estimate bioavailable steady-state contaminant concentrations (C(m(ss)) in aquatic systems. However, the validity of applying la...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/08-115.1

    authors: Raeside AA,O'Rourke SM,Drouillard KG

    更新日期:2009-02-01 00:00:00

  • Microcystin-LR modulates selected immune parameters and induces necrosis/apoptosis of carp leucocytes.

    abstract::Microcystins (MCs) are potent hepatotoxins acting by the inhibition of protein phosphatase 1 and 2A, and may promote liver tumors. Moreover, studies also suggest they are nephrotoxic. The aim of the present study was to assess possible in vitro effects of microcystin-LR (which contains the amino acids leucine and argi...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.87

    authors: Rymuszka A,Sierosławska A,Bownik A,Skowroński T

    更新日期:2010-03-01 00:00:00

  • An ecological risk assessment for triclosan in lotic systems following discharge from wastewater treatment plants in the United States.

    abstract::A modeling study was conducted to examine the distribution of concentrations of the antimicrobial triclosan (2,4,4'-trichloro-2'-hydroxydiphenyl ether) in rivers following discharge from wastewater treatment plants (WWTPs). Most uses of triclosan are disposed of down residential drains and ultimately reach WWTPs. A mo...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:

    authors: Reiss R,Mackay N,Habig C,Griffin J

    更新日期:2002-11-01 00:00:00

  • Toxic effects of oral hexahydro-1,3,5-trinitro-1,3,5-triazine in the western fence lizard (Sceloporus occidentalis).

    abstract::Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) has been widely used as an explosive in munition formulations, resulting in contamination of wildlife habitat on military installations. To estimate health effects for reptilian species, acute, subacute, and subchronic oral toxicity studies were conducted using the Western...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/08-419.1

    authors: McFarland CA,Quinn MJ Jr,Bazar MA,Talent LG,Johnson MS

    更新日期:2009-05-01 00:00:00

  • Utility of a juvenile fathead minnow screening assay for detecting (anti-)estrogenic substances.

    abstract::The European Chemical Industry's aquatic research program for endocrine disrupters includes the development of an in vivo juvenile fathead minnow (Pimephales promelas) screening assay. Working within the Organization for Economic Cooperation and Development's (OECD, Paris, France) tiered approach to endocrine disrupte...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:

    authors: Panter GH,Hutchinson TH,Länge R,Lye CM,Sumpter JP,Zerulla M,Tyler CR

    更新日期:2002-02-01 00:00:00

  • Decamethylcyclopentasiloxane (D5) environmental sources, fate, transport, and routes of exposure.

    abstract::The environmental sources, fate, transport, and routes of exposure of decamethylcyclopentasiloxane (D5; CAS no. 541-02-6) are reviewed in the present study, with the objective of contributing to effective risk evaluation and assessment of this and related substances. The present review, which is part of a series of st...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章,评审

    doi:10.1002/etc.2941

    authors: Mackay D,Cowan-Ellsberry CE,Powell DE,Woodburn KB,Xu S,Kozerski GE,Kim J

    更新日期:2015-12-01 00:00:00

  • Toxicity and bioaccumulation of biosolids-borne triclosan in terrestrial organisms.

    abstract::Triclosan (TCS) is a common constituent of personal care products and is frequently present in biosolids. Application of biosolids to land transfers significant amounts of TCS to soils. Because TCS is an antimicrobial and is toxic to some aquatic organisms, concern has arisen that TCS may adversely affect soil organis...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.1721

    authors: Pannu MW,O'Connor GA,Toor GS

    更新日期:2012-03-01 00:00:00

  • Improving substance information in USEtox® , part 2: Data for estimating fate and ecosystem exposure factors.

    abstract::The scientific consensus model USEtox® has been developed since 2003 under the auspices of the United Nations Environment Programme-Society of Environmental Toxicology and Chemistry Life Cycle Initiative as a harmonized approach for characterizing human and freshwater toxicity in life cycle assessment and other compar...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.3903

    authors: Saouter E,Aschberger K,Fantke P,Hauschild MZ,Kienzler A,Paini A,Pant R,Radovnikovic A,Secchi M,Sala S

    更新日期:2017-12-01 00:00:00

  • Spatial Distribution and Location of Natural Organic Matter on Sediment Particles by Scanning Electron Microscopic Analysis and the Development of a New Persistent Organic Pollutant-Sediment Kinetic Desorption Model.

    abstract::Natural organic matter (NOM) has long been shown to be the dominant factor in determining equilibrium and kinetic processes during sorption and desorption phenomena in sediment and soil experiments. Although several models have been suggested for predicting these processes, few offer mechanistic interpretations becaus...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.4915

    authors: Clay MM,Morgan JA,Dunnivant FM

    更新日期:2021-02-01 00:00:00

  • Implications of fluoroquinolone contamination for the aquatic environment-A review.

    abstract::Until recently, the behaviors of antibiotics and their ecotoxicological impact have been overlooked in the environment. The topic is broad and encompasses a wide range of organisms including microorganisms, algae, invertebrates, and vertebrates inhabiting various aquatic ecosystems. Changing the equilibrium of any 1 c...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章,评审

    doi:10.1002/etc.3552

    authors: Janecko N,Pokludova L,Blahova J,Svobodova Z,Literak I

    更新日期:2016-11-01 00:00:00

  • Pyrethroid insecticide concentrations and toxicity in streambed sediments and loads in surface waters of the San Joaquin Valley, California, USA.

    abstract::Pyrethroid insecticide use in California, USA, is growing, and there is a need to understand the fate of these compounds in the environment. Concentrations and toxicity were assessed in streambed sediment of the San Joaquin Valley of California, one of the most productive agricultural regions of the United States. Con...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.106

    authors: Domagalski JL,Weston DP,Zhang M,Hladik M

    更新日期:2010-04-01 00:00:00

  • Estimation of polychlorinated biphenyl fugacity ratios.

    abstract::On the quantitative comparison of solubilities or vapor pressures of homologous series, the variation in the effect of crystal structure on solid properties may substantially influence the outcome of the comparison. Usually, the effect of this variation is eliminated by comparing values of the liquid state. The ratio ...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/06-083r.1

    authors: Van Noort PC

    更新日期:2006-11-01 00:00:00

  • Photodegradation of the antihistamine cetirizine in natural waters.

    abstract::The photodegradation rate of the anti-histamine cetirizine (Zyrtec®) was investigated in various water matrices. The average observed first-order photodegradation rate coefficient (kobs ), obtained by linear regression of the logarithmic-transformed cetirizine concentrations versus irradiation time in simulated sunlig...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.2691

    authors: Mead RN,Barefoot S,Helms JR,Morgan JB,Kieber RJ

    更新日期:2014-10-01 00:00:00

  • Harbor seals (Phoca vitulina) in British Columbia, Canada, and Washington State, USA, reveal a combination of local and global polychlorinated biphenyl, dioxin, and furan signals.

    abstract::The harbor seal (Phoca vitulina) can serve as a useful indicator of food web contamination by persistent organic pollutants (POPs) because of its high trophic level, wide distribution in temperate coastal waters of the Northern Hemisphere, and relative ease of capture. In 1996 through 1997, we live-captured 60 harbor ...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/03-85

    authors: Ross PS,Jeffries SJ,Yunker MB,Addison RF,Ikonomou MG,Calambokidis JC

    更新日期:2004-01-01 00:00:00

  • Toxicity of substituted anilines to Pseudokirchneriella subcapitata and quantitative structure-activity relationship analysis for polar narcotics.

    abstract::This study evaluated the toxic effects of substituted anilines on Pseudokirchneriella subcapitata with the use of a closed algal toxicity testing technique with no headspace. Two response endpoints (i.e., dissolved oxygen production [DO] and algal growth rate) were used to evaluate the toxicity of anilines. Both DO an...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/06-293r.1

    authors: Chen CY,Ko CW,Lee PI

    更新日期:2007-06-01 00:00:00

  • Combined effects of temperature and pyriproxyfen stress in a full life-cycle test with Chironomus riparius (Insecta).

    abstract::Traditional risk assessment guidelines employ acute or chronic toxicity tests for a maximum of one generation of organisms. These tests are usually performed in the laboratory at a constant standard temperature, although in the field organisms may experience different temperatures, which may be a source of additional ...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.1969

    authors: Tassou KT,Schulz R

    更新日期:2012-10-01 00:00:00

  • Review of the reproductive biology of amphipods and their endocrine regulation: identification of mechanistic pathways for reproductive toxicants.

    abstract::The reproductive biology of amphipods is reviewed to update the knowledge of the male and female reproductive processes of oogenesis and spermatogenesis as well as the endocrine systems of amphipods with the aim of advancing studies of reproductive toxicology. The ovarian and reproduction cycles of female gammaridean ...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章,评审

    doi:10.1002/etc.673

    authors: Hyne RV

    更新日期:2011-12-01 00:00:00

  • Cytochrome P4501A biomarker indication of oil exposure in harlequin ducks up to 20 years after the Exxon Valdez oil spill.

    abstract::Hydrocarbon-inducible cytochrome P4501A (CYP1A) expression was measured, as ethoxyresorufin-O-deethylase (EROD) activity, in livers of wintering harlequin ducks (Histrionicus histrionicus) captured in areas of Prince William Sound, Alaska, USA, oiled by the 1989 Exxon Valdez spill and in birds from nearby unoiled area...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.129

    authors: Esler D,Trust KA,Ballachey BE,Iverson SA,Lewis TL,Rizzolo DJ,Mulcahy DM,Miles AK,Woodin BR,Stegeman JJ,Henderson JD,Wilson BW

    更新日期:2010-05-01 00:00:00

  • Plasma concentrations of organohalogenated pollutants in predatory bird nestlings: associations to growth rate and dietary tracers.

    abstract::The extent to which persistent organic pollutants (POPs) with different physicochemical properties originated from the food (dietary input) was assessed in raptor nestlings. Lipophilic polychlorinated biphenyl (PCB) 153, 1-dichloro-2,2-bis(p-chlorophenyl)ethylene (p,p'-DDE), and hexachlorobenzene (HCB), and protein-bo...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.2329

    authors: Bustnes JO,Bårdsen BJ,Herzke D,Johnsen TV,Eulaers I,Ballesteros M,Hanssen SA,Covaci A,Jaspers VL,Eens M,Sonne C,Halley D,Moum T,Nøst TH,Erikstad KE,Ims RA

    更新日期:2013-11-01 00:00:00

  • Understanding the atmospheric measurement and behavior of perfluorooctanoic acid.

    abstract::The recently reported quantification of the atmospheric sampling artifact for perfluorooctanoic acid (PFOA) was applied to existing gas and particle concentration measurements. Specifically, gas phase concentrations were increased by a factor of 3.5 and particle-bound concentrations by a factor of 0.1. The correlation...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.1932

    authors: Webster EM,Ellis DA

    更新日期:2012-09-01 00:00:00

  • Toxicity of extracts from municipal wastewater to early life stages of Japanese medaka (Oryzias latipes) to evaluate removals of micropollutants by wastewater treatment.

    abstract::Treatment of municipal wastewater reduces the concentrations of some pharmaceuticals and personal care products (PPCPs), hormones, and drugs of abuse. However, reduced concentrations of these micropollutants in wastewater may not correlate with reduced toxicity because transformations of micropollutants and/or the for...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.3939

    authors: Maya N,Crispo C,McFarland V,Nasuhoglu D,Isazadeh S,Yargeau V,Metcalfe CD

    更新日期:2018-01-01 00:00:00

  • Aerobic degradation of ethyl-tert-butyl ether by a microbial consortium: selection and evaluation of biodegradation ability.

    abstract::A microbial consortium that degrades ethyl-tert-butyl ether (ETBE) as the sole source of carbon and energy under aerobic conditions was selected from a gasoline-polluted soil. This consortium consists of a variety of microorganisms with a predominance of filamentous morphology. Degradation of ETBE was found to be sole...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:

    authors: Kharoune M,Kharoune L,Lebault JM,Pauss A

    更新日期:2002-10-01 00:00:00

  • Effects of spray drift of glyphosate on nontarget terrestrial plants-A critical review.

    abstract::Glyphosate is a widely used broad-spectrum postemergent herbicide used for weed control in both agricultural and nonagricultural settings. Spray drift of glyphosate can pose a risk to nontarget terrestrial plants and plant communities outside the intended area of application, but the lack of a well-established predict...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章,meta分析,评审

    doi:10.1002/etc.3925

    authors: Cederlund H

    更新日期:2017-11-01 00:00:00

  • The effects of a mixture of copper, nickel, and zinc on the structure and function of a freshwater planktonic community.

    abstract::It is generally assumed that as long as the majority of species experiences no direct adverse effects attributable to a single substance (i.e., potentially affected fraction [PAF] <5%), no significant structural or functional effects at the community level are expected to occur. Whether this assumption holds for mixed...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.4185

    authors: Van Regenmortel T,Van de Perre D,Janssen CR,De Schamphelaere KAC

    更新日期:2018-09-01 00:00:00

  • Bioaccumulation and biotransformation of polycyclic aromatic hydrocarbons during sediment tests with oligochaetes (Lumbriculus variegatus).

    abstract::In some kinetic studies with aquatic invertebrates, the bioaccumulation of polyaromatic hydrocarbons (PAHs) has been observed to peak at the beginning of the test. This has been explained by the depletion of PAHs from pore water due to limited desorption during the bioaccumulation test or, alternatively, by the activa...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/01-171.1

    authors: Lyytikäinen M,Pehkonen S,Akkanen J,Leppänen M,Kukkonen JV

    更新日期:2007-12-01 00:00:00