Inhibition of glutathione peroxidase and glutathione transferase in mouse liver by misonidazole.


:The mechanisms of toxicity and sensitization by the radiosensitizer misonidazole [1-(2-nitro-1-imidazolyl)-3-methoxy-2-propanol] are not well understood. We report here on the inhibition of total glutathione peroxidase (GSHPx), selenium-dependent glutathione peroxidase (selenium-GSHPx) and glutathione transferase (GSHTx) activities by misonidazole. Mouse liver cytosol GSHPx and selenium-GSHPx were inhibited in vitro with 0.5 mM misonidazole. On administration of the drug intraperitoneally (800 mg/kg) to mice, it was found that GSHPx, selenium-GSHPx, and GSHTx were inhibited in homogenate, cytosol, and microsomal fractions of mouse liver. GSHPx was depressed in all fractions up to 60-70% of control values, with maximum depression occurring in the cytosol and homogenate fractions in less than 2 hr. Recovery of activity was slower in the microsomes. In general, the pattern of depression of selenium-GSHPx was parallel to that of GSHPx except in microsomes, where GSHPx is minimal. Quantitatively, selenium-GSHPx was least affected. GSHTx was inhibited 70-80% of control values in cytosol and homogenate with recovery by 24 hr, whereas a second period of depression occurred at 24 hr in the microsomes. The inhibition of peroxide-metabolizing enzymes may lead to elevation of intracellular peroxide levels, contributing to the radiosensitizing effect and/or toxicity of misonidazole.


Biochem Pharmacol


Biochemical pharmacology


Kumar KS,Weiss JF




Has Abstract


1986-09-15 00:00:00














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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Pelorgeas S,Martin JB,Satre M

    更新日期:1992-12-01 00:00:00

  • Basement membrane components (matrigel) promote the tumorigenicity of human breast adenocarcinoma MCF7 cells and provide an in vivo model to assess the responsiveness of cells to estrogen.

    abstract::The ability to transplant human tumors into athymic nude mice allows studies of tumor cells in vivo. However, after s.c. injection the incidence of tumor and metastases in nude mice is frequently low. We have studied the tumorigenicity in nude mice of estradiol (E2)-sensitive breast adenocarcinoma MCF7 cells. Matrigel...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Noel A,Simon N,Raus J,Foidart JM

    更新日期:1992-03-17 00:00:00

  • Sp-2-propylthio-ATP-α-B and Sp-2-propylthio-ATP-α-B,β-γ-dichloromethylene are novel potent and specific agonists of the human P2Y₁₁ receptor.

    abstract::The human P2Y₁₁ nucleotide receptor mRNA was found in virtually all human tissues, and the receptor serves many physiological roles, such as immune response regulation. The Ala-87-Thr-P2Y₁₁ receptor single nucleotide polymorphism was linked to increased risk for acute myocardial infarction. To facilitate the developme...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Haas M,Ben-Moshe I,Fischer B,Reiser G

    更新日期:2013-09-01 00:00:00

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    abstract::We have shown previously that (R)-5-fluoro-5,6-dihydrouracil (FUraH(2)) attenuates the antitumor activity of 5-fluorouracil (FUra) in rats bearing advanced colorectal carcinoma. Presently, we found that alpha-fluoro-beta-alanine (FBAL), the predominant catabolite of FUra that is formed rapidly via FUraH(2), also decre...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Cao S,Baccanari DP,Rustum YM,Davis ST,Tansik RL,Porter DJ,Spector T

    更新日期:2000-04-15 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Young CS,Mason R,Hill SJ

    更新日期:1988-07-15 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章,评审


    authors: Terada T,Inui K

    更新日期:2008-05-01 00:00:00

  • Regulation of p53: intricate loops and delicate balances.

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章,收录出版


    authors: Oren M,Damalas A,Gottlieb T,Michael D,Taplick J,Leal JF,Maya R,Moas M,Seger R,Taya Y,Ben-Ze'ev A

    更新日期:2002-09-01 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Rakitina TV,Vasilevskaya IA,O'Dwyer PJ

    更新日期:2007-06-01 00:00:00

  • Comparison of the effects of recently developed alpha 2-adrenergic antagonists with yohimbine and rauwolscine on monoamine synthesis in rat brain.

    abstract::The effects of two recently developed alpha 2-adrenergic antagonists, RX 781094 and WY 26703, on the synthesis of norepinephrine (NE), dopamine (DA) and serotonin (5-HT) in rat brain were compared to those of yohimbine, its diastereoisomer rauwolscine, and mianserin. Intraperitoneal administration of these compounds i...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Pettibone DJ,Pfleuger AB,Totaro JA

    更新日期:1985-04-01 00:00:00

  • Alteration of rat liver endoplasmic reticulum Ca(2+)-ATPase thiol integrity by ciprofibrate, a peroxisome proliferator.

    abstract::Ciprofibrate (CP), a peroxisome proliferator, has been shown to reduce rat liver endoplasmic reticulum (ER) Ca(2+)-ATPase activity both in vitro and in vivo. The ER Ca(2+)-ATPase is highly susceptible to thiol reactivity, and maintenance of maximal enzyme activity is critically dependent upon the integrity of these th...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Bennett AM,Williams GM

    更新日期:1993-05-25 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Combes B,Backof B

    更新日期:1982-08-15 00:00:00

  • Induction of G(2)/M phase arrest and apoptosis by a new synthetic anti-cancer agent, DW2282, in promyelocytic leukemia (HL-60) cells.

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Piao W,Yoo J,Lee DK,Hwang HJ,Kim JH

    更新日期:2001-12-01 00:00:00

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    abstract::The effects of sulfobromophthalein (SBP) and ethacrynic acid (ECA), both inhibitors of glutathione S-transferase (GST), or glyceryl trinitrate (GTN)-induced vasorelaxation were investigated in rabbit aortic strips. The aortic strips were pre-contracted with phenylephrine, followed by relaxation with 0.5 microM GTN, wi...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Lau DT,Benet LZ

    更新日期:1992-05-28 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Thull U,Kneubühler S,Gaillard P,Carrupt PA,Testa B,Altomare C,Carotti A,Jenner P,McNaught KS

    更新日期:1995-09-07 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Matsumoto RM,Cho AK

    更新日期:1982-01-01 00:00:00

  • Effect of nerve growth factor and thyrotropin releasing hormone on cholinergic neurones in developing rat brain reaggregate cultures lesioned with ethylcholine mustard aziridinium.

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    pub_type: 杂志文章


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    更新日期:1989-05-15 00:00:00

  • Effects of chain length and sulphur position of thia fatty acids on their incorporation into phospholipids in 7800 C1 hepatoma cells and isolated rat hepatocytes, and their effects on fatty acid composition of phospholipids.

    abstract::Incorporation of thia fatty acids and their effects on the fatty acid composition in phospholipids has been investigated in 7800 C1 hepatoma cells and cultured hepatocytes. 3-Thia fatty acids of chain lengths from dodecyl-to hexadecyl-thioacetic acid were incorporated into phospholipids during a 3-day incubation. Long...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Wu P,Grav HJ,Horn R,Bremer J

    更新日期:1996-03-22 00:00:00

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    journal_title:Biochemical pharmacology

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    authors: Majumdar R,Balasubramanian AS

    更新日期:1985-12-01 00:00:00

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Yee LK,Allegra CJ,Trepel JB,Grem JL

    更新日期:1992-04-01 00:00:00

  • Differential effects of in vitro peroxidation on peripheral- and central-type benzodiazepine receptors. Protection by diverse antioxidants.

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Courtiere A,Molard F,Reybaud J

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Best L,Elliott AC,Brown PD

    更新日期:2007-06-01 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


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    更新日期:1988-12-01 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


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    更新日期:1989-06-01 00:00:00

  • Regulation of eicosanoid biosynthesis in the macrophage. Involvement of protein tyrosine phosphorylation and modulation by selective protein tyrosine kinase inhibitors.

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Glaser KB,Sung A,Bauer J,Weichman BM

    更新日期:1993-02-09 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Zheng Q,Li Q,Zhao G,Zhang J,Yuan H,Gong D,Guo Y,Liu X,Li K,Lin P

    更新日期:2020-10-01 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Herling AW,Becht M,Lang HJ,Scheunemann KH,Weidmann K,Scholl T,Rippel R

    更新日期:1990-10-15 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Drake JC,Hande KR,Fuller RW,Chabner BA

    更新日期:1980-03-01 00:00:00

  • Angiotensin II promotes pulmonary metastasis of melanoma through the activation of adhesion molecules in vascular endothelial cells.

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Ishikane S,Hosoda H,Nojiri T,Tokudome T,Mizutani T,Miura K,Akitake Y,Kimura T,Imamichi Y,Kawabe S,Toyohira Y,Yanagihara N,Takahashi-Yanaga F,Miyazato M,Miyamoto K,Kangawa K

    更新日期:2018-08-01 00:00:00