Abstract：
:Metallothionein (MT) levels were measured by radioimmunoassay in bronchoalveolar cells and fluids derived from rat lungs following 0, 8, 12, 18, or 24 exposures to Cd acetate aerosols. Alveolar cell metallothionein levels increased from a base line value of 0.35 micrograms MT/mg protein in control rats to a value of 21.73 micrograms MT/mg protein in rats exposed 24 times to Cd aerosols. Cell-free lavage fluid levels of metallothionein also increased in response to frequency of Cd exposure but were 5 to 10-fold lower than in the free alveolar cells. Gel chromatographic analysis of free alveolar cells from Cd-exposed animals revealed the presence of a 11,600-Da Cd-binding protein with an absorption maximum of 254 nm. Metallothionein analyses of purified populations of alveolar macrophages and polymorphonuclear leukocytes from exposed animals demonstrated that macrophages but not polymorphonuclear leukocytes contained metallothionein.
journal_name
Environ Resjournal_title
Environmental researchauthors
Hart BA,Garvey JSdoi
10.1016/s0013-9351(86)80114-1subject
Has Abstractpub_date
1986-08-01 00:00:00pages
391-8issue
2eissn
0013-9351issn
1096-0953pii
S0013-9351(86)80114-1journal_volume
40pub_type
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