Outward Na-K-Cl cotransport in human red blood cells: a methodological evaluation of method for epidemiologic investigations.

Abstract:

:The present report evaluates a method for the determination of the maximal velocity of the outward sodium, potassium, chlorine (Na-K-Cl) cotransport in erythrocytes. The loading procedure was carried out using the ionophore nystatin. The technical error for this assay, based on concurrent analysis of randomized blind duplicate samples, was 8.04% of the sample mean and 27.5% of the sample standard deviation. The assay was stable over time and reproducible. Fasting was not necessary. The ratio of intra- to interindividual variances was small. The cells can be safely preserved in an appropriate preservation solution without modification of the ion transport assay. The assay appears to be adequate for use in large-scale field trials.

journal_name

Am J Epidemiol

authors

Trevisan M,Canessa M,Laurenzi M,Di Muro MR,De Colle S,Franzese A,Iacone R,De Chiara F

doi

10.1093/oxfordjournals.aje.a114602

subject

Has Abstract

pub_date

1987-05-01 00:00:00

pages

860-8

issue

5

eissn

0002-9262

issn

1476-6256

journal_volume

125

pub_type

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