A rapid bioassay for screening rhizosphere microorganisms for their ability to induce systemic resistance.

Abstract:

:ABSTRACT We developed a rapid and miniaturized bioassay for screening large numbers of rhizosphere microorganisms for their ability to induce systemic resistance to bacterial leaf spot of radish caused by Xanthomonas campestris pv. armoraciae. In this bioassay, Pantoea agglomerans strain E278Ar controlled symptoms of disease as effectively as 2,6-dichloroisonicotinic acid when applied to the roots of seedlings produced in growth pouches in a soilless system. E278Ar essentially did not migrate from seedling roots to the foliage. This suggests that induction of systemic resistance could best explain the observed reduction in disease severity. Three mini-Tn5Km-induced mutants of strain E278Ar were isolated that had lost the ability to induce resistance. The bioassay also was used to demonstrate that the fungal biocontrol agent Trichoderma hamatum strain 382 induces systemic resistance in radish. The bioassay required only 14 to 18 days from seeding until rating for disease severity, which is 10 to 14 days less than earlier bioassays.

journal_name

Phytopathology

journal_title

Phytopathology

authors

Han DY,Coplin DL,Bauer WD,Hoitink HA

doi

10.1094/PHYTO.2000.90.4.327

subject

Has Abstract

pub_date

2000-04-01 00:00:00

pages

327-32

issue

4

eissn

0031-949X

issn

1943-7684

journal_volume

90

pub_type

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