Abstract:
BACKGROUND:Skeletal muscle from lean and obese subjects elicit differential adaptations in response to exercise/muscle contractions. In order to determine whether obesity alters the adaptations in mitochondrial dynamics in response to exercise/muscle contractions and whether any of these distinct adaptations are linked to alterations in insulin sensitivity, we compared the effects of electrical pulse stimulation (EPS) on mitochondrial network structure and regulatory proteins in mitochondrial dynamics in myotubes from lean humans and humans with severe obesity and evaluated the correlations between these regulatory proteins and insulin signaling. METHODS:Myotubes from human skeletal muscle cells obtained from lean humans (BMI 23.8 ± 1.67 kg/m) and humans with severer obesity (45.5 ± 2.26 kg/m) (n=8/group) were electrically stimulated for 24 hours. Four-hours after EPS, mitochondrial network structure, protein markers of insulin signaling and mitochondrial dynamics were assessed. RESULTS:EPS enhanced insulin-stimulated Akt phosphorylation, reduced the number of non-networked individual mitochondria and increased the mitochondrial network size in both groups (P<0.05). Mitochondrial fusion marker mitofusin 2 was significantly increased in myotubes from the lean subjects (P<0.05), but reduced in subjects with severe obesity (P<0.05). In contrast, fission marker dynamin-related protein 1 (Drp1) was reduced in myotubes from subjects with severe obesity (P<0.05), but remained unchanged in lean subjects. Reductions in Drp phosphorylation were correlated with improvements in insulin-stimulated Akt phosphorylation following EPS (r = -0.679, P = 0.004). CONCLUSION:Our data demonstrated that EPS induces more fused mitochondrial networks, which are associated with differential adaptations in mitochondrial dynamic processes in myotubes from lean humans and human with severe obesity. It also suggests that improved insulin signaling following muscle contractions may be linked to the reduction in Drp1 activity.
journal_name
Med Sci Sports Exercjournal_title
Medicine and science in sports and exerciseauthors
Kugler BA,Deng W,Francois B,Nasta M,Hinkley JM,Houmard JA,Gona PN,Zou Kdoi
10.1249/MSS.0000000000002580subject
Has Abstractpub_date
2020-12-11 00:00:00eissn
0195-9131issn
1530-0315pub_type
杂志文章abstract:PURPOSE:The purpose of this study was to investigate neuromuscular characteristics and muscle power as determinants of distance running performance. METHODS:Seventeen male endurance athletes performed a 5-km time trial (5K) that included three separate constant-velocity 200-m laps during the course and a maximal 20-m ...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:10.1097/00005768-199901000-00020
更新日期:1999-01-01 00:00:00
abstract::Increased vertical impact loading during running has been associated with a variety of running related injuries including stress fractures, patellofemoral pain, and plantar fasciitis. PURPOSE:The purpose of this study was to examine the acute and long-term effect of a gait retraining program aimed at teaching runners...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:10.1249/MSS.0000000000001710
更新日期:2018-12-01 00:00:00
abstract:BACKGROUND:Extracellular vesicles (EV) are purported to mediate type 2 diabetes and CVD risk and development. Physical activity and a balanced diet reduce disease risk, but no study has tested the hypothesis that short-term interval (INT) training would reduce EV compared with continuous (CONT) exercise in adults with ...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:10.1249/MSS.0000000000002185
更新日期:2020-03-01 00:00:00
abstract:UNLABELLED:Accurate assessments of both physical activity and sedentary behaviors are crucial to understand the health consequences of movement patterns and to track changes over time and in response to interventions. PURPOSE:The study evaluates the validity of an integrative, machine learning method for processing ac...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:10.1249/MSS.0000000000000915
更新日期:2016-08-01 00:00:00
abstract::It is the position of the American Dietetic Association, Dietitians of Canada, and the American College of Sports Medicine that physical activity, athletic performance, and recovery from exercise are enhanced by optimal nutrition. These organizations recommend appropriate selection of foods and fluids, timing of intak...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章,实务指引,评审
doi:10.1249/MSS.0b013e31890eb86
更新日期:2009-03-01 00:00:00
abstract:PURPOSE:Investigate hemodynamic responses of resistance exercise (RE) with moderate load (i.e., international guidelines for RE of patients) versus RE with high load in patients with coronary artery disease (CAD). METHODS:Medically stable male (n = 11) and female patients (n = 4) treated with PCI or percutaneous coron...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章,随机对照试验
doi:10.1249/MSS.0000000000000811
更新日期:2016-04-01 00:00:00
abstract::The case of a table tennis player suffering from intra-articular loose bodies of the elbow is reported. The patient developed the first signs of osteochondritis dissecans of the right lateral humeral condyle at age 16 but underwent surgery only 6 yr later. He has now resumed training and competition, despite some resi...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:
更新日期:1991-08-01 00:00:00
abstract::The influence of somatotype on the validity of anthropometric prediction of body density (Db) in young women (N = 92) was investigated. Three groups of predominantly endomorph (N = 27), mesomorph (N = 35), and ectomorph (N = 30) women were identified by the Heath-Carter and Sheldon somatotyping methods. Discriminant a...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:
更新日期:1984-08-01 00:00:00
abstract::A 23-yr-old female body builder injured her right wrist while training. A correct diagnosis of pure distal radio-ulnar dislocation, ulnar volar, was made, and reduction was successfully carried out. After removal of plaster, the patient complained of wrist pain and clicking. Surgical exploration revealed a ruptured tr...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00
abstract:PURPOSE:The purpose of this study was to evaluate physical performance (static and dynamic) of U.S. Air Force reservists after an eastbound air travel across seven time zones and to estimate the pharmacological aids slow-release caffeine and melatonin versus placebo in attempt to overcome the decline in performance. M...
journal_title:Medicine and science in sports and exercise
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00005768-200104000-00018
更新日期:2001-04-01 00:00:00
abstract:PURPOSE:The aim of this study was to investigate the influence of mouth rinsing a CHO-electrolyte (CHO-E) solution on 1-h running performance. A second study determined whether mouth rinsing a CHO-E solution altered the blood glucose and plasma insulin concentrations at rest. METHODS:After a 13-h fast, 10 endurance-tr...
journal_title:Medicine and science in sports and exercise
pub_type: 临床试验,杂志文章
doi:10.1249/MSS.0b013e3181bac6e4
更新日期:2010-04-01 00:00:00
abstract:INTRODUCTION:The significant sex disparity in sports-related knee injuries may be due to underlying differences in motor control. Although the development of sex-specific movement patterns is likely multifactorial, this study specifically focuses on the potential modulatory role of sex hormones. PURPOSE:This study aim...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:10.1249/MSS.0000000000000134
更新日期:2014-03-01 00:00:00
abstract:UNLABELLED:Nampt/pre-B-cell colony-enhancing factor/visfatin (visfatin) release from adipocytes has recently been suggested to be nutrient responsive and linked to systemic nicotinamide adenine dinucleotide biosynthesis and regulation of pancreatic beta-cell function. PURPOSE:We hypothesized that if visfatin does play...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:10.1249/MSS.0b013e318195bad5
更新日期:2009-06-01 00:00:00
abstract::Physical inactivity has been linked to rates of obesity, diabetes, and heart disease through insulin resistance and other mechanisms. Although sedentary workplace environments have unintentionally contributed to the risk for chronic diseases, innovations in the workplace environment could potentially rectify this publ...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:10.1249/MSS.0000000000001679
更新日期:2018-10-01 00:00:00
abstract::The relationships between individual performance and lactate exchange and removal abilities were studies in 12 male rowers all subjected to three measurements on a rowing ergometer. An incremental exercise carried out to determine the maximal oxygen uptake (VO2max) and the corresponding maximal aerobic power (Pamax), ...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:10.1097/00005768-199703000-00016
更新日期:1997-03-01 00:00:00
abstract:PURPOSE:Unilateral strength training strengthens not only the muscles on the trained side but also the homologous muscles on the untrained side; however, the magnitude of this interlimb cross-education is modest. We tested the hypothesis that heightened sensory feedback by mirror viewing the exercising hand would augme...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章,随机对照试验
doi:10.1249/MSS.0000000000000871
更新日期:2016-06-01 00:00:00
abstract::A group of 103 female weight lifters (WL) and 92 control (C) women answered a survey concerning eating behavior and attitudes (including the Eating Disorder Inventory) and menstrual function. The incidence of menstrual dysfunction, defined as oligomenorrhea plus amenorrhea, was significantly higher for the WL (30%) th...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract:PURPOSE:This investigation assessed the effects of a 9-wk regimen of creatine monohydrate (Cr x H2O) supplementation coupled with resistance training on body composition and neuromuscular performance in NCAA Division I football athletes. METHODS:Twenty-five subjects were randomly assigned in a double-blind, randomized...
journal_title:Medicine and science in sports and exercise
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00005768-200110000-00009
更新日期:2001-10-01 00:00:00
abstract:PURPOSE:The effects of chronic exercise training on the expression of heat shock protein (HSP) in skeletal muscle of senescent animals are unknown. Therefore, the purpose of this study was to investigate the effects of chronic exercise training on skeletal muscle HSP expression in both young and old rats. METHODS:Youn...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:10.1097/00005768-200105000-00008
更新日期:2001-05-01 00:00:00
abstract:PURPOSE:This study aimed to establish whether time spent in sport-related physical activities and sedentary behaviors (e.g., TV viewing, videogame usage, and homework) was prospectively associated with change in blood pressure (BP) for 5 yr. METHODS:Eight hundred and twenty-one students age 6 yr at baseline (397 girls...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:10.1249/MSS.0000000000000166
更新日期:2014-04-01 00:00:00
abstract:PURPOSE:To describe the level of usage of protective devices and equipment in a cohort of New Zealand rugby players. METHODS:Male and female players (N = 327) from a range of competitive grades were followed over the course of the season. Participants were interviewed weekly about their participation in rugby and use ...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:10.1097/00005768-200112000-00024
更新日期:2001-12-01 00:00:00
abstract:PURPOSE:To characterize the hemodynamic and ventilatory responses to exercise in a group of patients with unexplained dyspnea, increased risk for pulmonary arterial hypertension (PAH), and an elevated mean pulmonary artery pressure (mPAP; >30 mm Hg) on exercise. METHODS:A total of 37 symptomatic patients at risk of PA...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:10.1249/MSS.0b013e318204cdac
更新日期:2011-06-01 00:00:00
abstract::Vigorous exercise is associated with a sensation of well-being, and this subjective state has been objectively quantified with psychometric, cardiovascular, and neurophysiological data. Reductions in state anxiety have been demonstrated to follow acute physical activity, and this response persists for 2-5 h. Chronic p...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章,评审
doi:
更新日期:1985-02-01 00:00:00
abstract:PURPOSE:Aerobic training of professional road cyclists is linked to tremendous aerobic capacities that have never been clearly related to what occur in skeletal muscles submitted to a specific exercise. The aim of the present study was to examine specifically metabolic recovery after an incremental cycling exercise per...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:10.1249/01.mss.0000162616.20085.b4
更新日期:2005-05-01 00:00:00
abstract:PURPOSE:The purpose of this study was to provide baseline data on immune status of exercising and sedentary exclusively lactating women. Dietary intake and body composition were also investigated to determine whether they related to immune function. METHODS:Healthy, exclusively breastfeeding women with a body mass ind...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:10.1249/01.mss.0000128245.39808.36
更新日期:2004-06-01 00:00:00
abstract:PURPOSE:Development of an endurance training-overtraining protocol for Wistar rats that includes increased workload and is characterized by analyses of performance and biomarkers. METHODS:The running protocol lasted 11 wk: 8 wk of daily exercise sessions followed by 3 wk of increasing training frequency (two, three, a...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:10.1249/MSS.0b013e318191259c
更新日期:2009-05-01 00:00:00
abstract:INTRODUCTION:The application of an autologous plasma rich in growth factors is beneficial in restoring connective tissues, as shown by clinical evidence in oral surgery and more recently in arthroscopic anterior cruciate ligament reconstruction and two cases of ruptured Achilles tendon in professional athletes. This is...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:10.1249/01.MSS.0000089344.44434.50
更新日期:2003-10-01 00:00:00
abstract:INTRODUCTION:Loud sounds can decrease reaction time (RT) and increase force generated during voluntary contractions. Accordingly, we hypothesized that the loud starter's pistol at the Olympic Games allows runners closer to the starter to react sooner and stronger than runners farther away. METHODS:RT for the 100/110 m...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:10.1249/MSS.0b013e31816770e1
更新日期:2008-06-01 00:00:00
abstract::We examined whether the relation between of ratings of perceived exertion (RPE) and exercise intensities associated with the lactate threshold (LT) and blood lactate concentrations (BLC) of 2.5 and 4.0 mM, established with an incremental protocol, held during 30-min treadmill run at constant velocity (V). RPE (11.6, 1...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:10.1249/00005768-199406000-00021
更新日期:1994-06-01 00:00:00
abstract::Machine learning classification models for accelerometer data are potentially more accurate methods to measure physical activity in young children than traditional cut point methods. However, existing algorithms have been trained on laboratory-based activity trials, and their performance has not been investigated unde...
journal_title:Medicine and science in sports and exercise
pub_type: 杂志文章
doi:10.1249/MSS.0000000000002221
更新日期:2020-05-01 00:00:00