Abstract：
:Commercial nisin was encapsulated in nanovesicles (mean diameter 140 nm) prepared from partially purified soy lecithin. Nisin-loaded liposomes and unencapsulated (free) nisin were initially tested in BHI medium and skim milk inoculated with Listeria monocytogenes and incubated for 48 h at 30 degrees C. At such abuse temperature conditions, free nisin showed better inhibitory than the liposomal counterparts. Subsequently, the effect of encapsulated or free nisin was evaluated in combination with refrigeration (7 +/- 1 degrees C) in both whole (3.25% fat) and skim (0% fat) milk for up to 14 day. A decrease of 3-4 log cycles in L. monocytogenes counts was observed for free and encapsulated nisin at 0.5 mg/ml concentration. Liposome encapsulation of antimicrobial peptides may be important to overcome stability issues and interaction with food components. The utilization of nanovesicle-encapsulated nisin in combination with low temperatures appeared to be effective to control L. monocytogenes in milk, emphasizing the importance of hurdle technology to assure food safety.
journal_name
Food Microbioljournal_title
Food microbiologyauthors
da Silva Malheiros P,Daroit DJ,da Silveira NP,Brandelli Adoi
10.1016/j.fm.2009.09.013subject
Has Abstractpub_date
2010-02-01 00:00:00pages
175-8issue
1eissn
0740-0020issn
1095-9998pii
S0740-0020(09)00224-Xjournal_volume
27pub_type
杂志文章abstract：:Antibiotic-resistant enterococci are important opportunistic pathogens and have been recovered from retail tomatoes. However, it is unclear where and how tomatoes are contaminated along the farm-to-fork continuum. Specifically, the degree of pre-harvest contamination with enterococci is unknown. We evaluated the preva...
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pub_type： 杂志文章
doi：10.1016/j.fm.2008.12.010
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pub_type： 杂志文章
doi：10.1016/j.fm.2005.01.006
更新日期：2006-02-01 00:00:00
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doi：10.1016/j.fm.2012.12.011
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pub_type： 杂志文章,meta分析
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pub_type： 杂志文章
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更新日期：2013-10-01 00:00:00
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journal_title：Food microbiology
pub_type： 杂志文章
doi：10.1016/j.fm.2009.12.008
更新日期：2010-06-01 00:00:00
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更新日期：2016-08-01 00:00:00
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journal_title：Food microbiology
pub_type： 杂志文章,评审
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更新日期：2014-02-01 00:00:00
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journal_title：Food microbiology
pub_type： 杂志文章
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更新日期：2018-08-01 00:00:00
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更新日期：2020-08-01 00:00:00
