Fast identification of wine related lactic acid bacteria by multiplex PCR.

Abstract:

:The microflora of must and wine consists of yeasts, acetic acid bacteria and lactic acid bacteria (LAB). The latter group plays an important role for wine quality. The malolactic fermentation carried out by LAB leads to deacidification and stabilisation of wines. Nevertheless, LAB are often associated with wine spoilage. They are mainly responsible for the formation of biogenic amines. Furthermore, some strains produce exopolysaccharide slimes, acetic acid, diacetyl and other off-flavours. In this context a better monitoring of the vinification process is crucial to improve wine quality. Moreover, a lot of biodiversity studies would also profit from a fast and reliable identification method. In this study, we propose a species-specific multiplex PCR system for a rapid and simultaneous detection of 13 LAB species, frequently occurring in must or wine: Lactobacillus brevis, Lb. buchneri, Lb. curvatus, Lb. hilgardii, Lb. plantarum, Leuconostoc mesenteroides, Oenococcus oeni, Pediococcus acidilactici, P. damnosus, P. inopinatus, P. parvulus, P. pentosaceus and Weissella paramesenteroides.

journal_name

Food Microbiol

journal_title

Food microbiology

authors

Petri A,Pfannebecker J,Fröhlich J,König H

doi

10.1016/j.fm.2012.08.011

subject

Has Abstract

pub_date

2013-02-01 00:00:00

pages

48-54

issue

1

eissn

0740-0020

issn

1095-9998

pii

S0740-0020(12)00176-1

journal_volume

33

pub_type

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