Abstract：
:We have previously shown a relationship between the virulence level of Listeria monocytogenes strains and their detection on PALCAM medium. To account for the fact that only 40% of low-virulence field strains of L. monocytogenes were detected on PALCAM medium compared to 92% on ALOA medium, the detection of virulent and low-virulence strains on decomposed selective ALOA and PALCAM media was compared. This showed that better detection of the strains was not explained by the growth factors added to the ALOA medium. On the other hand, the presence of acriflavine in the PALCAM medium partly explained the delay in detection of the low-virulence strains, while the presence of ceftazidime was related to growth inhibition. However, the effect of these two components was modified when they were combined in the PALCAM medium. As some of these low-virulence strains had an inactive PrfA (the transcriptional activator of the main virulence genes of L. monocytogenes), its role in the poor detection of these low-virulence strains was investigated. However, complementing these strains with the wild-type prfA gene or deleting the prfA gene from a virulent strain suggested that this poor detection was unrelated to PrfA, but was related to their higher susceptibility to the antimicrobial components in the selective media.
journal_name
Food Microbioljournal_title
Food microbiologyauthors
Roche SM,Gracieux P,Velge Pdoi
10.1016/j.fm.2008.08.001subject
Has Abstractpub_date
2009-02-01 00:00:00pages
21-6issue
1eissn
0740-0020issn
1095-9998pii
S0740-0020(08)00144-5journal_volume
26pub_type
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