Effect of freezing on lens epithelial cell growth.

Abstract:

:The effect of freezing on the growth of rat lens epithelial cells was studied in vitro. We found that 80% of the lens epithelial cells died after freezing at -45 degrees C for two hours and that the surviving cells could grow with the addition of growth factors or when placed on a sheet of type 4 collagen, but not when placed on a plain plastic culture dish. These results suggest that the surviving cells are at the Go phase of the cell cycle and that type 4 collagen or growth factors can initiate cell division.

authors

Fukaya Y,Hara T,Hara T,Iwata S

doi

10.1016/s0886-3350(88)80122-6

subject

Has Abstract

pub_date

1988-05-01 00:00:00

pages

309-11

issue

3

eissn

0886-3350

issn

1873-4502

pii

S0886-3350(88)80122-6

journal_volume

14

pub_type

杂志文章
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