Abstract:
OBJECTIVE:To research the expression and biological function of micro ribonucleic acid (miR)-645 in non-small cell lung cancer (NSCLC), and to further explore the regulatory relationship between miR-645 and tumor protein p53 inducible protein 11 (TP53I11). PATIENTS AND METHODS:A total of 41 tissue samples were collected from NSCLC patients, and RNAs were extracted from these tissues and reversely transcribed. Then, the expression level of miR-645 in the 41 tissue samples of patients, as well as that in NSCLC cells and human bronchial mucosal epithelial cells, was detected by quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR). In vitro functional assays [methyl thiazolyl tetrazolium (MTT) assay, colony formation assay and transwell assay] were conducted to explore the effects of miR-645 on the proliferation and migration abilities of NSCLC cells. Finally, the downstream target genes of miR-645 were predicted by bioinformatics, screened via qRT-PCR and Western blotting experiments, and verified through Dual-Luciferase reporter gene assay. RESULTS:QRT-PCR results showed that the miR-645 expression was upregulated in the tissue samples of 35 out of 41 NSCC cases. Besides, the miR-645 expression was upregulated in NSCC cells compared with that in human bronchial mucosal epithelial cells. After interfering with miR-645 expression, in vitro functional assay (MTT assay, colony formation assay and transwell assay) results revealed that the cell proliferation, migration, and invasion were inhibited. According to the results of qRT-PCR and Western blotting, after knocking down the expression of miR-645 in NSCLC cells, the expression of TP53I11 was upregulated, and the results of Dual-Luciferase reporter gene assay confirmed that miR-645 could directly bind to TP53I11. CONCLUSIONS:MiR-645 expression is upregulated in NSCLC tissues and cells, and the proliferation and migration of NSCLC cells are promoted by targeted regulation on the TP53I11 expression.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Zhu B,Tian T,Zhao Mdoi
10.26355/eurrev_202006_21510subject
Has Abstractpub_date
2020-06-01 00:00:00pages
6150-6156issue
11eissn
1128-3602issn
2284-0729journal_volume
24pub_type
杂志文章abstract:OBJECTIVE:To explore the role of Id1 in ovarian cancer cell proliferation, invasion and apoptosis. MATERIALS AND METHODS:Lentivirus-based shRNA vectors were constructed to knockdown Id1 expression in SKOV3 ovarian cancer cells. The proliferation, invasion ability and apoptosis of SKOV3 cells were evaluated by CCK-8 as...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-07-01 00:00:00
abstract:OBJECTIVE:This paper aims to investigate the interaction mechanism between pioglitazone/simvastatin and the CD40-CD40 ligand (CD40-CD40L) system and to determine their interaction effects on atherosclerosis in rabbits. MATERIALS AND METHODS:Forty rabbits were randomly divided into five groups of eight: normal control,...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:The objective of this case report is to describe the management of anesthesia of a patient with an LVAD that underwent an emergency open appendectomy. Literature regarding emergency anesthesia management of such patients is still limited. A search in the PubMed engine with the keywords "LVAD appendectomy anes...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-08-01 00:00:00
abstract::The article "Evaluation of cytotoxicity of some common ophthalmic drugs" by M. Li, X.-M. Chen, J.-J. Liu, D.-M. Wang, L. Gan, X. Lv, Y. Qiao, published in Eur Rev Med Pharmacol Sci 2015; 19 (11): 1945-1950 has been withdrawn. ...
journal_title:European review for medical and pharmacological sciences
pub_type: 撤回出版物
doi:
更新日期:2015-09-01 00:00:00
abstract::Catheter-related bloodstream infections (CRBI) represent a frequent complication of immune-compromised hosts with a high mortality rate. In this setting, opportunistic pathogens can create a biofilm on implanted devices, being the source of infection. We provide a mini-review of the literature, starting from the descr...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2015-07-01 00:00:00
abstract:OBJECTIVE:Sunitinib is a multiple tyrosine kinase inhibitor (TKI) that exerts anti-tumor and antiangiogenic activity. It is used for the treatment of metastatic gastrointestinal stromal tumours, renal cell carcinoma and pancreatic neuroendocrine tumours. A few studies confirm the anti-tumour activity of sunitinib in br...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVE:Mesenchymal stem cells are a population of pluripotent cells that can differentiate into epidermal-like cells under certain conditions. Wnt3a can promote the proliferation and differentiation of stem cells. However, the role of Wnt3a in the differentiation of human umbilical cord mesenchymal stem cells (hUCMS...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
abstract::Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, "Long non-coding RNA DANCR upregulates IGF2 expression and promotes ovarian cancer progression, by Y.-Q. Gao, H.-Y. Cheng, K.-F. Liu, published in Eur Rev ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202009_22998
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVE:Determination of the active fraction and compounds of the dichloromethanol extract of Schinus molle seeds and evaluation of their biological effects. MATERIALS AND METHODS:Dried seeds of Schinus molle were sequentially extracted in hexane, acetyl acetate and dichloromethane. The dichloromethane extract was s...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
abstract::Allergic bronchopulmonary aspergillosis (ABPA) occurs in 7-9% of patients with cystic fibrosis (CF) and causes a worsening of lung function and respiratory symptoms. Standard treatment of ABPA consists of oral steroids; however, higher corticosteroid therapy associated to antifungal agent (itraconazole) long-term trea...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2014-07-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the effect of long non-coding ribonucleic acid (lncRNA) growth arrest specific 5 (GAS5) on acute myocardial infarction (AMI) model rats and to explore its regulatory mechanism. MATERIALS AND METHODS:The rat model of AMI was established by subcutaneous injection of iso...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201910_19173
更新日期:2019-10-01 00:00:00
abstract:OBJECTIVES:Diabetes mellitus (DM) causes organ dysfunction and increases the sensitivity of organs to damages.To test this hypothesis, we used renal ischemia/reperfusion (I/R) experiment to evaluate the renoprotective activity of telmisartan versus pioglitazone on I/R induced renal damage in diabetic rats. MATERIALS A...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2012-05-01 00:00:00
abstract::Macrodistrophia Lipomatosa is a congenital malformation of rare finding and unknown pathogenic mechanism. The pathology is mainly characterized by the interest of lower extremities and peculiar macroscopic feature is the presence of hyperthrophic fibro-adipose tissue. In our experience the localization to the upper ex...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00
abstract:OBJECTIVE:Long-term survival of patients with neonatal-onset carbamoyl-phosphate synthetase 1 deficiency (CPS1D), an autosomal recessive disorder characterized by repeated, life-threatening hyperammonemia, is rare. We describe the diagnosis and clinical management of a teenager with neonatal-onset CPS1D who did not und...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202010_23220
更新日期:2020-10-01 00:00:00
abstract:OBJECTIVE:Menin, encoded by the Men1 gene, is responsible for β-cell tumor formation in patients with multiple endocrine neoplasia type 1. Recently, Menin has been proven to negatively regulate β-cell proliferation in several mouse models, including hyperglycemia. However, it is unclear how glucose regulates Menin expr...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-02-01 00:00:00
abstract:OBJECTIVE:Long noncoding RNAs (lncRNAs) have been well concerned in tumor researches, which are believed to influence tumorigenesis and tumor progression. This study aims to uncover the role of LINC00707 in clear cell renal cell carcinoma (ccRCC) and the underlying mechanism. MATERIALS AND METHODS:Differentially expre...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202006_21647
更新日期:2020-06-01 00:00:00
abstract::Pneumatosis intestinalis is an uncommon condition characterized by the presence of gas in the bowel wall. We present the case of a 49-year-old man admitted to our Clinic for his 4 day long haematochezia. Colonoscopy revealed pneumatosis coli as a cause of the lower gastrointestinal bleeding. A wide range of diagnostic...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2013-09-01 00:00:00
abstract:OBJECTIVE:This study aims to investigate whether circ-VANGL1 can promote the progression of osteoporosis (OP) by absorbing miRNA-217 to regulate RUNX2 expression. PATIENTS AND METHODS:The serum levels of circ-VANGL1, miRNA-217 and RUNX2 in OP patients and non-OP patients were detected by quantitative Real Time-Polymer...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201902_16981
更新日期:2019-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The aim of this study was to examine potential relationship between subjective sleep quality and degree of pain in patients with shoulder impingement syndrome (SIS). MATERIALS AND METHODS:Fourty patients with shoulder impingement syndrome were evaluated using the Pittsburgh Sleep Quality Inde...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2013-02-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to observe the association between N-myc downstream regulated gene 2 (NDRG2)/interleukin-6 (IL-6)/signal transducer and activator of transcription 3 (STAT3) signaling pathway and diabetic retinopathy (DR) in rats. MATERIALS AND METHODS:The model of diabetes was successfully establis...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202004_20806
更新日期:2020-04-01 00:00:00
abstract:OBJECTIVE:Glioma is characterized by high metastasis with poor outcomes. Long non-coding RNA metastasis associated lung adenocarcinoma transcript 1 (MALAT1) was well-explored in numerous human cancers, including glioma. This study aimed to provide a novel action mechanism of MALAT1 in glioma. MATERIALS AND METHODS:The...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202003_20529
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVES:CHARGE is an acronym referring to the aspects of this rare syndromic condition. Patients with CHARGE association are today considered as subjects lacking in pathognomonic dental alterations. The present study is aimed at adding to the body of evidence of the cases reported in literature and the continuous cl...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:To explore the protective role of Vitamin D Receptor (VDR) in retinal ganglion cells (RGCs) after high-level glucose induction, and to investigate its underlying mechanism. MATERIALS AND METHODS:Primary RGCs were isolated from 24-hour-old Sprague Dawley (SD) rats and cultured in 50 mmol/L glucose. The expres...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201811_16292
更新日期:2018-11-01 00:00:00
abstract:OBJECTIVE:Crohn's disease (CD) is a chronic inflammatory bowel disease that can affect the entire gastrointestinal tract. Cardiac involvement is considered very rare. Pericarditis, myocarditis, endocarditis, cardiomyopathy and complete heart block are some of the cardiac extraintestinal manifestations of CD. The aim of...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-07-01 00:00:00
abstract:OBJECTIVE:To clarify the function of miRNA-153-3p in gefitinib-sensitive non-small cell lung cancer (NSCLC) and the underlying mechanism. PATIENTS AND METHODS:The expressions of miRNA-153-3p, LC3B and ATG5 in gefitinib-resistant and gefitinib-sensitive NSCLC tissues were determined by quantitative Real Time-Polymerase...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201903_17391
更新日期:2019-03-01 00:00:00
abstract:BACKGROUND:Hepatic fibrosis is characterised by a progressive accumulation of fibrillar extracellular matrix (ECM) proteins, including collagen that occurs in chronic liver diseases. Transforming growth factor-beta1 (TGF-beta)/Smad3 signalling plays a major role in tissue fibrogenesis acting as a potent stimulus of ECM...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2012-10-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to explore the relationship between the mitogen-activated protein kinase (MAPK)/extracellular regulated protein kinase (ERK) pathway and neurocyte apoptosis after cerebral infarction in rats. MATERIALS AND METHODS:Neural stem cells were isolated from rats by establishing the cerebra...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201906_18206
更新日期:2019-06-01 00:00:00
abstract:OBJECTIVE:The purpose of this study was to explore the role of microRNA-32 (miR-32) in ovarian cancer and the possible underlying mechanism. PATIENTS AND METHODS:Ovarian cancer tissues were collected from 100 patients diagnosed with ovarian cancer in our hospital. Quantitative Real-Time Polymerase Chain Reaction (qRT-...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202005_21154
更新日期:2020-05-01 00:00:00
abstract:BACKGROUND:Several elimination diets have been suggested based on results of skin prick tests (SPTs) or IgE antibodies to foods, thus allowing the identification of the most common offending food(s), including CM (cow's milk), egg, peanut and wheat. But unbalanced, inappropriate dietary manipulation in infants with foo...
journal_title:European review for medical and pharmacological sciences
pub_type: 临床试验,杂志文章
doi:
更新日期:2006-03-01 00:00:00
abstract:OBJECTIVE:To explore the safety and efficiency of transvaginal surgical treatment of cesarean scar pregnancy (CSP). PATIENTS AND METHODS:A retrospective analysis of 54 CSP patients that received treatment in our hospital from October 2011 to September 2015 was performed, dividing two groups: Group A (n=34) received tr...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-06-01 00:00:00