Abstract：
OBJECTIVE:Mesenchymal stem cells are a population of pluripotent cells that can differentiate into epidermal-like cells under certain conditions. Wnt3a can promote the proliferation and differentiation of stem cells. However, the role of Wnt3a in the differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs) into epidermal-like cells is unknown. MATERIALS AND METHODS:Third-generation hUCMSCs were cultured in normal medium, epidermal stem cell-conditioned medium, and conditioned medium with added Wnt3a. After culturing for 5 days, the expression of cytokeratin 19 (CK19), an antigen specific for epidermal-like cells, was assessed by immunofluorescence and flow cytometry. The expression of CK19 mRNA was confirmed by reverse transcription-polymerase chain reaction(RT-PCR), and β-catenin expression was detected by western blot. RESULTS:hUCMSCs differentiated into epidermal-like cells when cultured in conditioned medium as shown by positive immunofluorescence staining for CK19. Flow cytometry showed that the number of cells positive for CK19 in the epidermal stem cell-conditioned medium group was significantly higher than that of control group, but lower than that of the Wnt3a-conditioned group (p < 0.05). RT-PCR showed that the expression level of CK19 mRNA in the conditioned medium group was significantly lower than that of the Wnt3a group (p < 0.01). Westernblots showed that the expression of β-catenin in the conditioned medium group was significantly lower than that of the Wnt3a group (p < 0.01). CONCLUSIONS:These results suggest that Wnt3a can effectively promote the differentiation of hUCMSCs into epidermal-like cells.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Sun TJ,Tao R,Han YQ,Xu G,Liu J,Han YFsubject
Has Abstractpub_date
2015-01-01 00:00:00pages
86-91issue
1eissn
1128-3602issn
2284-0729journal_volume
19pub_type
杂志文章abstract：OBJECTIVE:The aim of this study was to investigate the effect of long non-coding ribonucleic acid (lncRNA) growth arrest specific 5 (GAS5) on acute myocardial infarction (AMI) model rats and to explore its regulatory mechanism. MATERIALS AND METHODS:The rat model of AMI was established by subcutaneous injection of iso...
journal_title：European review for medical and pharmacological sciences
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pub_type： 临床试验,杂志文章
doi：
更新日期：2000-09-01 00:00:00
