Abstract:
:The evolution of resistance to drugs and pesticides poses a major threat to human health and food security. Neonicotinoids are highly effective insecticides used to control agricultural pests. They target the insect nicotinic acetylcholine receptor and mutations of the receptor that confer resistance have been slow to develop, with only one field-evolved mutation being reported to date. This is an arginine-to-threonine substitution at position 81 of the nAChR_β1 subunit in neonicotinoid-resistant aphids. To validate the role of R81T in neonicotinoid resistance and to test whether it may confer any significant fitness costs to insects, CRISPR/Cas9 was used to introduce an analogous mutation in the genome of Drosophila melanogaster. Flies carrying R81T showed an increased tolerance (resistance) to neonicotinoid insecticides, accompanied by a significant reduction in fitness. In comparison, flies carrying a deletion of the whole nAChR_α6 subunit, the target site of spinosyns, showed an increased tolerance to this class of insecticides but presented almost no fitness deficits.
journal_name
Mol Ecoljournal_title
Molecular ecologyauthors
Homem RA,Buttery B,Richardson E,Tan Y,Field LM,Williamson MS,Emyr Davies TGdoi
10.1111/mec.15503subject
Has Abstractpub_date
2020-07-01 00:00:00pages
2661-2675issue
14eissn
0962-1083issn
1365-294Xjournal_volume
29pub_type
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