Abstract:
:G-protein-coupled receptors (GPCRs) represent a major class of receptors through which a number of signals ranging from photons to large glycoprotein hormones are recognized. Human genome encodes about 800 GPCRs, yet very little structural information is available on this class of receptors. Structural studies provide a wealth of information about not only the activation mechanism of the receptor but also the crucial information about the ligand-binding pocket which could lead to the development of subtype-specific ligands. The crystal structure of human adenosine A(2A) receptor was solved in complex with a high-affinity antagonist ZM241385 at 2.6Å resolution. Here, we describe the methods that were undertaken to solve the fusion protein structure.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Ashok Y,Nanekar RT,Jaakola VPdoi
10.1016/B978-0-12-391861-1.00008-3subject
Has Abstractpub_date
2013-01-01 00:00:00pages
175-98eissn
0076-6879issn
1557-7988pii
B978-0-12-391861-1.00008-3journal_volume
520pub_type
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