Abstract:
:Paralytic shellfish toxins are secondary metabolites produced by several species of dinoflagellates and cyanobacteria. Known targets of these toxins, which typically occur at detrimental concentrations during harmful algal blooms, include voltage-gated ion channels in humans and other mammals. However, the effects of the toxins on the co-occurring phytoplankton community remain unknown. The present study examined the molecular mechanisms of the model photosynthetic alga Chlamydomonas reinhardtii in response to saxitoxin exposure as a means of gaining insight into the phytoplankton community response to a bloom. Previous work with yeast indicated that saxitoxin inhibited copper uptake, so experiments were designed to examine whether saxitoxin exhibited a similar mode of action in algae. Expression profiling following exposure to saxitoxin or a copper chelator produced similar profiles in copper homeostasis genes, notably induction of the cytochrome c6 (CYC6) and copper transporter (COPT1, CTR1) genes. Cytochrome c6 is used as an alternative to plastocyanin under conditions of copper deficiency, and immunofluorescence data showed this protein to be present in a significantly greater proportion of saxitoxin-exposed cells compared to controls. Live-cell imaging with a copper-sensor probe for intracellular labile Cu(I) confirmed that saxitoxin blocked copper uptake. Extrapolations of these data to phytoplankton metabolic processes along with the copper transporter as a molecular target of saxitoxin based on existing structural models are discussed.
journal_name
Environ Toxicol Chemjournal_title
Environmental toxicology and chemistryauthors
Cusick KD,Wetzel RK,Minkin SC Jr,Dodani SC,Wilhelm SW,Sayler GSdoi
10.1002/etc.2187subject
Has Abstractpub_date
2013-06-01 00:00:00pages
1388-95issue
6eissn
0730-7268issn
1552-8618journal_volume
32pub_type
杂志文章abstract::Diclofenac is a nonsteroidal anti-inflammatory drug (NSAID) that has been detected widely in surface waters in North America and Europe. The impact of diclofenac on river biofilm communities was investigated at exposures of 10 and 100 microg L(-1) of diclofenac or its molar equivalent in carbon and nutrients. Experime...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/06-340r.1
更新日期:2007-04-01 00:00:00
abstract::Breeding pairs of mallards (Anas platyrhynchos) were fed a control diet or a diet containing 0.5 microg/g mercury (Hg) in the form of methylmercury chloride. There were no effects of Hg on adult weights and no overt signs of Hg poisoning in adults. The Hg-containing diet had no effect on fertility of eggs, but hatchin...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.64
更新日期:2010-03-01 00:00:00
abstract::In the present study, electron paramagnetic resonance coupled with spin-trapping technique was used, with alpha-phenyl-N-tert-butylnitrone (PBN) as a spin-trapping agent, to investigate free radical generation in freshwater fish with acute 2,4-dichlorophenol (2,4-DCP) poisoning. The PBN-radical adducts were detected i...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/04-640r.1
更新日期:2005-09-01 00:00:00
abstract::Rice is the main crop in the subbasin of the fluvial lagoon system of Palizada River (FLSPR) in the state of Campeche, Mexico. The pesticides used to control pests of this crop mainly are carbofuran, chlorpyrifos, and glyphosate. Black-bellied whistling duck (Dendrocygna autumnalis) is an ecologically and economically...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/03-646.1
更新日期:2005-02-01 00:00:00
abstract::In tissues of freshwater fish-feeding mammals, 3,4-didehydroretinol (A2) is a major form of vitamin A. In mink liver, with organochlorine exposure, this analog has been found to decrease more than retinol (A1) and thus has potential as a sensitive freshwater biomarker. The presence of the analogs A1 and A2 as alcohol ...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:
更新日期:2002-02-01 00:00:00
abstract::Seedlings of Brassica juncea (L.) Czern. were grown in solution culture for 14 d prior to exposure to Pb₂+ at an activity of 31 microM for 72 h. Electron dense deposits found within the apoplast and symplast were analysed using scanning transmission electron microscopy-energy dispersive spectroscopy (STEM-EDS) to dete...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/09-131.1
更新日期:2009-11-01 00:00:00
abstract::Until recently, the behaviors of antibiotics and their ecotoxicological impact have been overlooked in the environment. The topic is broad and encompasses a wide range of organisms including microorganisms, algae, invertebrates, and vertebrates inhabiting various aquatic ecosystems. Changing the equilibrium of any 1 c...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章,评审
doi:10.1002/etc.3552
更新日期:2016-11-01 00:00:00
abstract::Hepatic vitellogenin (VTG) mRNA and plasma protein assays were developed for the Murray rainbowfish (Melanotaenia fluviatilis), a native freshwater fish species in Australia. Adult male Murray rainbowfish were exposed to 17β-estradiol (E2) or 17α-ethynylestradiol (EE2) at 0, 1, 5, 10, 50, or 100 ng/L in a semistatic s...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.660
更新日期:2011-11-01 00:00:00
abstract::Soot, soot-amended sediment, and unamended sediment spiked with hydrophobic organic contaminants (HOC) were subjected to laboratory desorption and assimilation efficiency experiments in an effort to assess and compare the importance of soot in controlling HOC desorption and deposit-feeder assimilation efficiency. Thre...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/03-351a
更新日期:2004-11-01 00:00:00
abstract::Recent studies have indicated the potential for dietborne metals as an important exposure pathway for metal toxicity in freshwater organisms. We conducted a study in which freshwater cladocerans (Ceriodaphnia dubia) were fed green algae (either Pseudokirchneriella subcapitata or Chlorella vulgaris) that were grown in ...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/07-587.1
更新日期:2009-01-01 00:00:00
abstract::The present study reports a 48-h aquatic metal-toxicity assay based on daily growth rates of the red seaweed Gracilaria domingensis (Gracilariales, Rhodophyta) in synthetic seawater. The median inhibitory concentration (IC50) for each metal cation was experimentally determined, and the ratios of free ions (aqueous com...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.2340
更新日期:2013-11-01 00:00:00
abstract::Manufactured gas plant (MGP) tar and wastewater solids historically were discharged into the Penobscot River, Maine,USA, via a sewer at the Bangor Landing site. The tar and wastewater solids accumulated in riverbed sediment over a 5-hectare area downstream from the sewer outfall. Much of the tarry sediment is a harden...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/08-603.1
更新日期:2009-11-01 00:00:00
abstract::Northern elephant seal (Mirounga angustirostris) pups undergo a substantial intertissue reorganization of protein, minerals, and other cellular components during their postweaning development, which might entail the mobilization of associated contaminants. The authors investigated the changes in concentrations of 11 e...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.1960
更新日期:2012-10-01 00:00:00
abstract::Crude oils contain a mixture of hydrocarbons, including phototoxic polycyclic aromatic hydrocarbons (PAHs) that have the ability to absorb ultraviolet (UV) light. Absorption of UV light by PAHs can substantially increase their toxicity to marine organisms. The objective of the present study was to examine the potentia...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.3808
更新日期:2017-10-01 00:00:00
abstract::Modulations of 11 prospective biochemical markers of impacts of aquatic pollutants in liver tissue of chub (Leuciscus cephalus), caught at several sampling sites of a river with various pollution types and rates, were matched against analytical data of concentrations of organochlorine compounds, polycyclic aromatic hy...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:
更新日期:2001-05-01 00:00:00
abstract::A meta-analysis was conducted of studies that reported behavior and chemo/mechanosensory responses by fish, amphibians, and aquatic invertebrates in Cu-containing waters and also reported sufficient water chemistry for calculation of hardness-based and biotic ligand model (BLM)-based water quality criteria (WQC) for C...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章,评审
doi:10.1002/etc.4096
更新日期:2018-05-01 00:00:00
abstract::Mercury contamination in wetland biota is often dynamic, difficult to predict, and costly to track. In this paper, we present results from a six-year study of growing feathers of piscivorous birds as monitors of wetland Hg exposure in Florida, USA, wetlands. Between 1994 and 2000, we collected feathers of growing grea...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::There is global concern regarding the fate and effects of microplastics in the environment, particularly in aquatic systems. In the present study, ethylene acrylic acid copolymer particles were evaluated in a chronic toxicity study with the aquatic invertebrate Daphnia magna. The study design included a natural partic...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.4745
更新日期:2020-08-01 00:00:00
abstract::Flexible, rapid, and predictive approaches that do not require the use of large numbers of vertebrate test animals are needed because the chemical universe remains largely untested for potential hazards. Development of robust new approach methodologies and nontesting approaches requires the use of existing information...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.4382
更新日期:2019-05-01 00:00:00
abstract::The time taken for organisms to recover from a pulsed toxicant exposure is an important consideration when water quality guidelines are applied to intermittent events in the environment. Organisms may appear to have recovered by standard toxicity testing methods but could carry residual toxicant or damage that may mak...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.2966
更新日期:2015-08-01 00:00:00
abstract::Genetic diversity among laboratory and field populations of Chironomus riparius was investigated using microsatellite DNA analysis. Individuals of midge cultures reared in 10 different laboratories showed a clear reduction in the number of alleles per locus and in the level of heterozygosity compared to two natural po...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/06-349r.1
更新日期:2007-05-01 00:00:00
abstract::The radiotracer technique was used to assess the influences of sediment-metal contact time or aging (up to two years) on the bioavailability and geochemical speciation of Cd and Zn in sediments. Bioavailability was quantified by measuring the assimilation efficiency of metals in two deposit-feeding invertebrates (sipu...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/06-166.1
更新日期:2006-10-01 00:00:00
abstract::The uptake of Se by the freshwater alga Chlamydomonas reinhardtii and the subsequent transfer to the Asiatic clam Corbicula fluminea was investigated. The objective was to investigate the bioavailability of algal-bound Se for C. fluminea while taking into account Se speciation and bivalve ventilation. First, uptake ra...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/05-386r1.1
更新日期:2006-10-01 00:00:00
abstract::The reference method for the Canada-wide standard (CWS) for petroleum hydrocarbons (PHCs) in soil provides laboratories with methods for generating accurate and reproducible soil analysis results. The CWS PHC tier 1 generic soil-quality guidelines apply to 4 carbon ranges/fractions: F1 (C6-C10), F2 (C10-C16), F3 (C16-...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.2578
更新日期:2014-08-01 00:00:00
abstract::The N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) is extensively used as pH buffer in culture media for testing chemicals. However, this study demonstrates that 0.01 M HEPES significantly reduces the rate of Cu, Pb, and Cd binding to Porphyra spp. and Enteromorpha spp. marine macroalgae. The HEPES also d...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:
更新日期:2002-02-01 00:00:00
abstract::A Unit World approach has been used extensively to rank chemicals for their hazards and to understand differences in chemical behavior. Whereas the fate and effects of an organic chemical in a Unit World Model (UWM) analysis vary systematically according to one variable (fraction of organic carbon), and the chemicals ...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.606
更新日期:2011-09-01 00:00:00
abstract::During the last four years, significant effort has been devoted to understanding the effects that Hurricanes Katrina and Rita had on contaminant distribution and redistribution in New Orleans, Louisiana, USA, and the surrounding Gulf Coast area. Elevated concentrations were found for inorganic contaminants (including ...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.205
更新日期:2010-07-01 00:00:00
abstract::We quantified the uncertainty due to biota-related parameters in estimated bioaccumulation factors (BAFs) of persistent organic pollutants for fish through Monte Carlo simulations. For this purpose, the bioaccumulation model OMEGA (Optimal Modeling for EcotoxicoloGical Applications) was parameterized based on data fro...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.393
更新日期:2011-02-01 00:00:00
abstract::The recently reported quantification of the atmospheric sampling artifact for perfluorooctanoic acid (PFOA) was applied to existing gas and particle concentration measurements. Specifically, gas phase concentrations were increased by a factor of 3.5 and particle-bound concentrations by a factor of 0.1. The correlation...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.1932
更新日期:2012-09-01 00:00:00
abstract::We measured concentrations of polychlorinated biphenyls (PCBs), polychlorinated dibenzo-p-dioxins (PCDDs), and polychlorinated dibenzofurans (PCDFs) in northern leopard frogs collected from the Green Bay ecosystem and explored the catalytic activity of hepatic cytochrome P450-associated monooxygenase (P450 enzyme) as ...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.5620181002
更新日期:1999-10-01 00:00:00