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Atrial fibrillation promotion by endurance exercise: demonstration and mechanistic exploration in an animal model.

Abstract：

OBJECTIVES:The goal of this study was to assess mechanisms underlying atrial fibrillation (AF) promotion by exercise training in an animal model. BACKGROUND:High-level exercise training promotes AF, but the underlying mechanisms are unclear. METHODS:AF susceptibility was assessed by programmed stimulation in rats after 8 (Ex8) and 16 (Ex16) weeks of daily 1-h treadmill training, along with 4 and 8 weeks after exercise cessation and time-matched sedentary (Sed) controls. Structural remodeling was evaluated by using serial echocardiography and histopathology, autonomic nervous system with pharmacological tools, acetylcholine-regulated potassium current (IKACh) with patch clamp recording, messenger ribonucleic acid expression with quantitative polymerase chain reaction, and regulators of G protein-signaling (RGS) 4 function in knockout mice. RESULTS:AF inducibility increased after 16 weeks of training (e.g., AF >30 s in 64% of Ex16 rats vs 15% of Sed rats; p < 0.01) and rapidly returned to baseline levels with detraining. Atropine restored sinus rhythm in 5 of 5 Ex rats with AF sustained >15 min. Atrial dilation and fibrosis developed after 16 weeks of training and failed to fully recover with exercise cessation. Parasympathetic tone was increased in Ex16 rats and normalized within 4 weeks of detraining. Baroreflex heart rate responses to phenylephrine-induced blood pressure elevation and IKACh sensitivity to carbachol were enhanced in Ex16 rats, implicating both central and end-organ mechanisms in vagal enhancement. Ex rats showed unchanged cardiac adrenergic and cholinergic receptor and IKACh-subunit gene expression, but significant messenger ribonucleic acid downregulation of IKACh-inhibiting RGS proteins was present at 16 weeks. RGS4 knockout mice showed significantly enhanced sensitivity to AF induction in the presence of carbachol. CONCLUSIONS:Chronic endurance exercise increased AF susceptibility in rats, with autonomic changes, atrial dilation, and fibrosis identified as potential mechanistic contributors. Vagal promotion is particularly important and occurs via augmented baroreflex responsiveness and increased cardiomyocyte sensitivity to cholinergic stimulation, possibly due to RGS protein downregulation.

journal_name

J Am Coll Cardiol

journal_title

Journal of the American College of Cardiology

authors

Guasch E,Benito B,Qi X,Cifelli C,Naud P,Shi Y,Mighiu A,Tardif JC,Tadevosyan A,Chen Y,Gillis MA,Iwasaki YK,Dobrev D,Mont L,Heximer S,Nattel S

doi

10.1016/j.jacc.2013.01.091

subject

Has Abstract

pub_date

2013-07-02 00:00:00

pages

68-77

issue

eissn

0735-1097

issn

1558-3597

pii

S0735-1097(13)01408-3

journal_volume

pub_type

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Atrial fibrillation promotion by endurance exercise: demonstration and mechanistic exploration in an animal model.