Abstract：
PURPOSE:This study aimed to assess impact of different vehicles for laser-assisted skin drug delivery. We also tried to uncover the mechanisms by which different vehicles affect laser-aided skin permeation. METHODS:Full-surface ablative (conventional) and fractional lasers were used to irradiate nude mouse skin. Imiquimod and 5-aminolevulinic acid (ALA) were used as lipophilic and hydrophilic permeants. Vehicles employed included water with 40% polyethylene glycol 400 (PEG 400), propylene glycol (PG), and ethanol. Lipid nanoparticles were also utilized as carriers. RESULTS:In vitro permeation profiles showed improvement in imiquimod flux with conventional laser (2.5 J/cm2) producing a 12-, 9-, and 5-fold increase when loading imiquimod in 40% PEG400, PG, and ethanol, respectively, as compared with intact skin. Nanoparticulate delivery by laser produced a 6-fold enhancement in permeation. Fractional laser produced less enhancement of imiquimod delivery than conventional laser. Laser exposure increased follicular imiquimod accumulation from 0.80 to 5.81 μg/cm2. ALA permeation from aqueous buffer, PEG 400, and PG with conventional laser treatment was 641-, 445-, and 104-fold superior to passive control. In vivo skin deposition of topically applied ALA examined by confocal microscopy indicated the same trend as the in vitro experiment, with aqueous buffer showing the greatest proporphyllin IX signaling. Diffusion of cosolvent molecules into ablated skin and drug partitioning from vehicle to skin are two predominant factors controlling laser-assisted delivery. In contrast to conventional laser, lateral drug diffusion was anticipated for fractional laser. CONCLUSIONS:Our results suggest that different drug delivery vehicles substantially influence drug penetration enhanced by lasers.
journal_name
Pharm Resjournal_title
Pharmaceutical researchauthors
Lee WR,Shen SC,Aljuffali IA,Li YC,Fang JYdoi
10.1007/s11095-013-1167-4subject
Has Abstractpub_date
2014-02-01 00:00:00pages
382-93issue
2eissn
0724-8741issn
1573-904Xjournal_volume
31pub_type
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journal_title：Pharmaceutical research
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journal_title：Pharmaceutical research
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journal_title：Pharmaceutical research
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journal_title：Pharmaceutical research
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journal_title：Pharmaceutical research
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doi：10.1023/a:1012164520429
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journal_title：Pharmaceutical research
pub_type： 杂志文章,评审
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journal_title：Pharmaceutical research
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更新日期：1991-04-01 00:00:00
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journal_title：Pharmaceutical research
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更新日期：2020-01-28 00:00:00
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journal_title：Pharmaceutical research
pub_type： 杂志文章
doi：10.1023/a:1011973409022
更新日期：1998-01-01 00:00:00
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更新日期：2003-06-01 00:00:00
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journal_title：Pharmaceutical research
pub_type： 杂志文章
doi：10.1023/a:1018995124527
更新日期：1999-12-01 00:00:00
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journal_title：Pharmaceutical research
pub_type： 杂志文章
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更新日期：2007-06-01 00:00:00
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journal_title：Pharmaceutical research
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更新日期：2012-08-01 00:00:00
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journal_title：Pharmaceutical research
pub_type： 杂志文章
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更新日期：2018-02-08 00:00:00
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journal_title：Pharmaceutical research
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更新日期：2008-09-01 00:00:00
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journal_title：Pharmaceutical research
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更新日期：2021-01-06 00:00:00
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journal_title：Pharmaceutical research
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更新日期：2003-08-01 00:00:00
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journal_title：Pharmaceutical research
pub_type： 杂志文章
doi：10.1023/b:pham.0000045247.09724.26
更新日期：2004-10-01 00:00:00
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journal_title：Pharmaceutical research
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更新日期：2006-05-01 00:00:00
