A rat model for long-gap peripheral nerve reconstruction.

Abstract:

BACKGROUND:The rat model has had limited utility for the study of long nerve gaps because of the small size of the animal. The authors sought to develop a simple, effective rat model for reconstruction of long nerve gap defects. METHODS:Fifteen rats had a sciatic nerve transection followed by reconstruction. Positive control rats received a 1-cm isograft. Negative control rats received a 3.5-cm hollow silicone conduit, and experimental rats received a 4-cm isograft; these were implanted in a looped configuration to accommodate the long length. Nerves were harvested at 6 weeks (1-cm grafts) and 12 weeks (3.5-cm conduits and 4-cm grafts) for histologic and histomorphometric evaluation. RESULTS:The 1-cm and 4-cm isograft groups showed robust regeneration in the distal nerve segment. The 3.5-cm hollow conduits showed absolutely no initiation of nerve regeneration. Histomorphometric values were as expected for the specified gap length. CONCLUSIONS:This study describes a simple and effective long nerve gap rat model for experiments on nerve grafts and nerve conduits. The long nerve graft model can be useful for studying techniques such as processed nerve grafts, which are currently a topic of frequent investigation. The 3.5-cm hollow conduit "no-regrowth" long-gap model is ideal for investigating conduit-based tissue-engineering solutions for long-gap nerve repair. The authors' approach overcomes the size limitation of the small animal while exploiting the features that make the rat the model of choice for preliminary nerve studies.

journal_name

Plast Reconstr Surg

authors

Vasudevan S,Yan JG,Zhang LL,Matloub HS,Cheng JJ

doi

10.1097/PRS.0b013e31829fe515

subject

Has Abstract

pub_date

2013-10-01 00:00:00

pages

871-876

issue

4

eissn

0032-1052

issn

1529-4242

pii

00006534-201310000-00025

journal_volume

132

pub_type

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