Competition between pyrimorph-sensitive and pyrimorph-resistant isolates of Phytophthora capsici.

Abstract:

:Phytophthora capsici causes significant losses to vegetable production worldwide. Pyrimorph, a new carboxylic acid amide fungicide, has been registered to control P. capsici in China. A mutation (Q1077K) in cellulose synthase 3 has been reported to confer resistance to pyrimorph. In this study, we measured the competition between pyrimorph-resistant and pyrimorph-sensitive isolates of P. capsici. Mixed zoospore suspensions of resistant (R) and sensitive (S) isolates at five ratios (1R:9S, 3R:7S, 5R:5S, 7R:3S, and 9R:1S) were applied to carrot agar in vitro test (with five successive transfers) and to the soil surface around pepper plants in planta test (with 10 successive disease cycles). The proportion of resistant isolates was measured by a conventional assay in which single zoospore isolates recovered after transfers or disease cycles were grown on agar medium with a discriminatory concentration of pyrimorph. The results were then compared with those of a real-time polymerase chain reaction (PCR)-based method developed here, the results were similar. Both assays showed that the competitive ability of the resistant isolates was similar to or less than that of the sensitive isolates. The real-time PCR assay developed will be useful for high-throughput analysis and monitoring the development of pyrimorph resistance in field populations of P. capsici.

journal_name

Phytopathology

journal_title

Phytopathology

authors

Pang Z,Shao J,Hu J,Chen L,Wang Z,Qin Z,Liu X

doi

10.1094/PHYTO-07-13-0185-R

subject

Has Abstract

pub_date

2014-03-01 00:00:00

pages

269-74

issue

3

eissn

0031-949X

issn

1943-7684

journal_volume

104

pub_type

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