Stage-related expression of mRNAs during pollen development in lily and tobacco.

Abstract:

:Homogeneous populations of developing microspores and pollen from anthers of lily (Lilium longiflorum Thumb.) and tobacco (Nicotiana tabacum L.) show a continuous production of biomass, reaching a maximum in young pollen. The rate of RNA synthesis was 460 fg · h(-1) in young binucleate cells, 138 fg · h(-1) in late binucleate cells and 56 fg · h(-1) in microspores. The mRNA population in developing pollen can be separated into three groups. In the first group, certain types of mRNAs are present at a constant level during all stages of development. A second group is characteristic of young pollen and increases quantitatively until anthesis. A third group is seen transiently; to this belong mRNAs present only before mitosis or at a distinct cell stage after mitosis. Some of the translation products of this latter group of mRNAs showed similarities between lily and tobacco on two-dimensional gels in respect of molecular weight and isolectric point, indicating that those mRNAs and proteins play a role in the regulation of pollen development.

journal_name

Planta

journal_title

Planta

authors

Schrauwen JA,de Groot PF,van Herpen MM,van der Lee T,Reynen WH,Weterings KA,Wullems GJ

doi

10.1007/BF00197125

subject

Has Abstract

pub_date

1990-09-01 00:00:00

pages

298-304

issue

2

eissn

0032-0935

issn

1432-2048

journal_volume

182

pub_type

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