Abstract:
INTRODUCTION:Culture-independent methods, based on bacterial 16 S rRNA gene sequencing, have been used previously to investigate the adenotonsillar microbiota. However, these studies have focused on a single sampling site (usually a surface swab). We aimed to investigate potential differences in adenotonsillar microbiota according to sampling location, both on and within the adenoids and palatine tonsils. METHODS:Pediatric patients (n = 28, mean age five years) undergoing adenotonsillectomy were recruited for this study. At the time of surgery, a mucosal adenoid surface swab and an adenoid tissue biopsy was collected. Immediately following surgery, the crypts of the right and left tonsils were swabbed, and a surface and core tissue sample from the right tonsil were also collected. Bacterial 16 S rRNA gene-targeted amplicon sequencing was used to determine the bacterial composition of the collected samples. RESULTS:There was no significant difference in diversity or composition of the adenoid microbiota based on sampling site. However, the Shannon-Wiener and Inverse-Simpson diversity indices differed significantly (p < 0.05) between the microbial communities of the three different tonsil sampling sites. There was a higher average relative abundance of members from the genera Streptococcus, Actinobacillus, and Neisseria in the tonsil crypts when compared with surface and core tonsil tissue samples. CONCLUSION:Our results indicate that there is variation in bacterial diversity and composition based on sampling sites in the tonsils but not the adenoids. The difference in microbiota between the surface and the tissue may have implications for our understanding of the pathogenesis of recurrent tonsillitis and have treatment implications.
journal_name
Int J Pediatr Otorhinolaryngoljournal_title
International journal of pediatric otorhinolaryngologyauthors
Johnston J,Hoggard M,Biswas K,Astudillo-García C,Radcliff FJ,Mahadevan M,Douglas RGdoi
10.1016/j.ijporl.2018.07.024subject
Has Abstractpub_date
2018-10-01 00:00:00pages
51-57eissn
0165-5876issn
1872-8464pii
S0165-5876(18)30339-2journal_volume
113pub_type
杂志文章abstract::A case of neonatal respiratory distress due to an oropharyngeal hairy polyp is reported with its MRI assessment. The place of hairy polyps in the differential diagnosis of upper respiratory tract obstruction is discussed. Early and rapid diagnosis and treatment may be lifesaving for the newborn. ...
journal_title:International journal of pediatric otorhinolaryngology
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更新日期:1990-06-01 00:00:00
abstract:OBJECTIVE:1.) Describe demographic and clinical characteristics of pediatric nasal septal perforations (NSP), 2.) Analyze efficacy of treatment modalities in symptom management and resolution of pediatric NSP, 3.) Describe the surgical technique of external rhinoplasty with vascularized nasal septal flaps in the treatm...
journal_title:International journal of pediatric otorhinolaryngology
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abstract:UNLABELLED:American Indian children have three times the rate of otitis media compared to the general population, yet prospective cohort studies of OME and hearing loss have not been previously reported in American Indian infants. Between 1997 and 2003, a cohort of 421 infants was enrolled at birth from Minnesota Ameri...
journal_title:International journal of pediatric otorhinolaryngology
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abstract::Four cases of laryngeal dysfunction are presented. The substantial advantages derived from the use of flexible fiberoptic instruments in children is described, together with a description of 3 scopes developed by the author. ...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
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更新日期:1983-07-01 00:00:00
abstract:OBJECTIVE:Acute otitis media (AOM) is one of the most common infectious diseases in children. Nontypeable Haemophilus influenzae (NTHi) is Gram-negative bacteria that are considered major pathogens of AOM and respiratory tract infections. In this study, we used monophosphoryl lipid A (MPL), a toll-like receptor (TLR) 4...
journal_title:International journal of pediatric otorhinolaryngology
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doi:10.1016/j.ijporl.2017.03.018
更新日期:2017-06-01 00:00:00
abstract::Second branchial cleft cysts and sinuses rarely present diagnostic problems to the pediatric otolaryngologist as their course is usually predictable based on consistent embryologic development. However, we evaluated two fistula tracts that did not fit the classic description of second branchial tract fistulas. Upon ra...
journal_title:International journal of pediatric otorhinolaryngology
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doi:10.1016/s0165-5876(98)00077-9
更新日期:1998-11-15 00:00:00
abstract::Evoked otoacoustic emissions using click stimulus (cEOEs) were recorded in 450 consecutive children over a 12-month period. Of this group, 102 were babies treated in neonatal intensive care unit (NICU), classified as High Risk neonates according to the criteria of the Joint Committee on Infant Hearing. Audiologic exam...
journal_title:International journal of pediatric otorhinolaryngology
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doi:10.1016/s0165-5876(97)01491-2
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abstract::Neonatal rhinitis as a distinct disease entity has not been well-studied. The recognition and treatment of this condition is important since neonates are obligate nasal breathers, and mismanagement of this entity can result in poor feeding or even death from respiratory distress. We undertook a retrospective analysis ...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/S0165-5876(96)01464-4
更新日期:1997-02-14 00:00:00
abstract:OBJECTIVE:The main objective of the present study was to investigate subcortical auditory processing in children with sensorineural hearing loss. METHODS:Auditory Brainstem Responses (ABRs) were recorded using click and speech/da/stimuli. Twenty-five children, aged 6-14 years old, participated in the study: 13 with no...
journal_title:International journal of pediatric otorhinolaryngology
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doi:10.1016/j.ijporl.2017.05.010
更新日期:2017-08-01 00:00:00
abstract:INTRODUCTION:A staging system for assessment of recurrent respiratory papillomatosis (RRP) has been in use over the last 3 years for 17 patients at our children's hospital. OBJECTIVE:To evaluate a staging system for assessment of RRP on the basis of the predictive value on the surgical interval of: anatomic staging sy...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2004.06.007
更新日期:2004-12-01 00:00:00
abstract::This prevalence study of secretory otitis media (SOM) comprised 210 children who were not attending or had not attended kindergarten. Children in home care/private day care with up to two children were analyzed separately. In this group the prevalence peak was at one year, being at that age about 40%. The prevalence d...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/s0165-5876(86)80013-1
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abstract::A total of 163 children were entered into a case-control study to determine whether any causal relationship exists between otitis media with effusion (OME) requiring grommet insertion and parental smoking. One hundred children with persistent OME formed the case group and 63 children with normal ears formed the contro...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/0165-5876(92)90062-t
更新日期:1992-07-01 00:00:00
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journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2010.02.002
更新日期:2010-04-01 00:00:00
abstract::A case of cerebral venous infarction (CVI) as a complication of acute otitis media (AOM) was presented in a 16-month-old male patient. The patient admitted with AOM in the right ear, ipsilateral facial paralysis and contralateral hemiplegia. Computerized tomography of the brain showed low density areas involving both ...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/s0165-5876(03)00181-2
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journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/s0165-5876(00)00355-4
更新日期:2000-08-31 00:00:00
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journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2016.10.001
更新日期:2016-12-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to assess the long term results of treatment and rehabilitation of childhood dysphonia. METHODS:This study included a group of adolescents (n=29) aged from 15 to 20 who were treated due to pediatric hyperfunctional dysphonia and soft vocal fold nodules during their pre-mutational pe...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2014.02.002
更新日期:2014-05-01 00:00:00
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journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2012.04.014
更新日期:2012-08-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2012-01-01 00:00:00
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journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2012.09.013
更新日期:2012-12-01 00:00:00
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journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2005.07.014
更新日期:2006-03-01 00:00:00
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journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章,多中心研究
doi:10.1016/j.ijporl.2007.08.012
更新日期:2007-12-01 00:00:00
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journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
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更新日期:2020-04-01 00:00:00
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journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2019.02.022
更新日期:2019-05-01 00:00:00
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journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2009.08.020
更新日期:2009-12-01 00:00:00
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journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2019.05.003
更新日期:2019-09-01 00:00:00
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journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2007.03.017
更新日期:2007-07-01 00:00:00
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journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2016.11.002
更新日期:2017-01-01 00:00:00
abstract::We present two siblings with severe laryngomalacia requiring surgical intervention during the newborn period, microcephaly, developmental delay, cleft palate, preaxial polydactyly, dysplastic nails and conductive hearing loss (persistent after tympanostomy tube placement). In addition the girl has microopthalmia and t...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章,评审
doi:10.1016/j.ijporl.2006.04.010
更新日期:2006-09-01 00:00:00
abstract:BACKGROUND:The distribution of congenital neck masses varies between countries and is important in diagnosis and treatment modalities. Data from Africa is scarce, and altogether absent from Kenya. OBJECTIVE:To describe the pattern of congenital masses in a Kenyan paediatric population. SET UP: Kenyatta National Hospit...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2009.10.012
更新日期:2010-01-01 00:00:00