Abstract:
:Primary cilia are microtubule-based, dynamic organelles characterized by continuous assembly and disassembly. The intraflagellar transport (IFT) machinery, including IFT88 in cilia, is involved in the maintenance of bidirectional motility along the axonemes, which is required for ciliogenesis and functional competence. Cancer cells are frequently associated with loss of primary cilia and IFT functions. However, there is little information on the role of IFT88 or primary cilia in the metabolic remodeling of cancer cells. Therefore, we investigated the cellular and metabolic effects of the loss-of-function (LOF) mutations of IFT88/primary cilia in thyroid cancer cells. IFT88-deficient 8505C thyroid cancer cells were generated using the CRISPR/Cas9 system, and RNA-sequencing analysis was performed. LOF of the IFT88 gene resulted in a marked defect in ciliogenesis and mitochondrial oxidative function. Gene expression patterns in IFT88-deficient thyroid cancer cells favored glycolysis and lipid biosynthesis. However, LOF of IFT88/primary cilia did not promote thyroid cancer cell proliferation, migration, and invasion. The results suggest that IFT88/primary cilia play a role in metabolic reprogramming in thyroid cancer cells.
journal_name
Oncogenejournal_title
Oncogeneauthors
Lee J,Yi S,Won M,Song YS,Yi HS,Park YJ,Park KC,Kim JT,Chang JY,Lee MJ,Sul HJ,Choi JE,Kim KS,Kero J,Kim J,Shong Mdoi
10.1038/s41388-018-0211-6subject
Has Abstractpub_date
2018-08-01 00:00:00pages
4455-4474issue
32eissn
0950-9232issn
1476-5594pii
10.1038/s41388-018-0211-6journal_volume
37pub_type
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