Abstract:
:Terence Kealey first pioneered the isolation and organ maintenance of human eccrine and sebaceous glands in the early to mid-1980. This led to subsequent methods describing the isolation and culture of human hair follicles, the human pilosebaceous unit as well as the sebaceous duct. The importance of these models in the study of the biology of human skin glands and appendages has been demonstrated in numerous publications and their importance as models for animal replacement, refinement and reduction (3Rs) is increasingly important. In particular, in vitro (ex vivo) hair follicle culture has played a significant part in helping elucidate the role of signalling molecules in regulating hair growth and hair fibre formation and has been especially useful in understanding metabolic aspects of hair growth. However, obtaining sufficient numbers of hair follicles is becoming increasingly difficult as plastic surgery becomes less invasive and smaller skin samples provided. There is therefore an urgent requirement for the next generation of in vitro models using cell lines and tissue engineering, and this has led to the development of immortalised cell lines as well as attempts to model hair follicle embryogenesis in vitro and development of skin on a chip.
journal_name
Exp Dermatoljournal_title
Experimental dermatologyauthors
Philpott MPdoi
10.1111/exd.13669subject
Has Abstractpub_date
2018-05-01 00:00:00pages
571-577issue
5eissn
0906-6705issn
1600-0625journal_volume
27pub_type
杂志文章,评审abstract::The expression of amphiregulin (AR) in the basal epidermis of transgenic mice [keratin 14 promoter AR gene (K14-ARGE)] has been previously shown to induce an early-onset and severe skin pathology, with many similarities to psoriasis. In this study, it is demonstrated that involucrin enhancer/promoter-dependent express...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.0906-6705.2004.00183.x
更新日期:2004-06-01 00:00:00
abstract::Hair loss (alopecia) can result from a variety of metabolic, endocrine, immunologic, and environmental causes. This investigation was undertaken to determine the mechanisms underlying the sporadic development of alopecia in litters from C57BL/6 interleukin-10-deficient (Il10(-/-)) mice. All pups in affected litters de...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.2009.01032.x
更新日期:2010-06-01 00:00:00
abstract::Pachyonychia congenita type 1 (PC-1) is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy, focal non-epidermolytic palmoplantar keratoderma and variable features of oral leukokeratosis and follicular keratosis. Previously, we have shown that this disease can be caused by mutations...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.1999.tb00356.x
更新日期:1999-04-01 00:00:00
abstract::Neutrophil extravasation is central to inflammatory skin diseases like psoriasis and atopic dermatitis. In vivo, neutrophils have been shown to migrate through cell-to-cell junctions (paracellular pathway) or directly through the body of the endothelial cell (transcellular pathway). In vitro, however, neutrophil migra...
journal_title:Experimental dermatology
pub_type: 信件
doi:10.1111/j.1600-0625.2008.00796.x
更新日期:2009-01-01 00:00:00
abstract::Vitamin D-binding protein (VDBP) single nucleotide polymorphisms (SNP) may affect skin carcinogenesis. The objective was to test the association between two functional VDBP SNPs and the susceptibility to (multiple) basal cell carcinomas (BCCs). Of the 7983 participants, 5790 (72.5%) and 5823 (72.9%) participants were ...
journal_title:Experimental dermatology
pub_type: 信件
doi:10.1111/j.1600-0625.2010.01139.x
更新日期:2010-12-01 00:00:00
abstract::Cutaneous wound pain causes physical and psychological stress for patients with wounds. Previous studies reported that stress induces hyperalgesia and deteriorates wound healing. However, the effect of the stress response such as in hypothalamic-pituitary-adrenal (HPA) axis on local wound area is unclear. We aimed to ...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.13994
更新日期:2019-09-01 00:00:00
abstract::Reverse transcription polymerase chain reaction (qRT-PCR) has become a mainstay in many areas of skin research. To enable quantitative analysis, it is necessary to analyse expression of reference genes (RGs) for normalization of target gene expression. The selection of reliable RGs therefore has an important impact on...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.12759
更新日期:2015-10-01 00:00:00
abstract:BACKGROUND:This study investigated predictors of response to topical diphenylyclopropenone (DPCP) immunotherapy in patients with alopecia areata (AA). OBJECTIVE:To identify predictors of response, or resistance, to treatment for AA through clinical observations and serum tests. METHODS:Eighty four AA patients were tr...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.13758
更新日期:2020-03-01 00:00:00
abstract::Chronic exposure to ultraviolet (UV) radiation alters components of the skin. We previously reported that chronic low-dose UV irradiation induced wrinkle formation but did not significantly alter dermal components of hairless mice. In the present study, we examined whether the epidermal changes by UV irradiation could...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1034/j.1600-0625.12.s2.4.x
更新日期:2003-01-01 00:00:00
abstract::The ethanolic extract of Resina Draconis (RDEE) has been reported beneficial to normal wound healing yielding more regularly arranged collagen fibres. Loureirin B, a major component in RDEE, has been supposed to be effective on the prevention and treatment of pathological scars. To investigate the therapeutic effects ...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.12665
更新日期:2015-05-01 00:00:00
abstract::Epidermal gene therapy may benefit a variety of inherited skin disorders and certain systemic diseases. Both in vivo and ex vivo approaches of gene transfer have been used to target human epidermal stem cells and achieve long-term transgene expression in immunodeficient mouse/human chimera models. Immunological respon...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.2005.00351.x
更新日期:2005-10-01 00:00:00
abstract::Chimeric antigen receptor (CAR)-T cells are one of the impressive recent success stories of anti-cancer immunotherapy. Especially in haematological malignancies, this treatment strategy has shown promising results leading to the recent approval of two CAR-T cell constructs targeting CD19 in the United States and the E...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.13792
更新日期:2018-12-01 00:00:00
abstract::For almost a quarter of a century, ex vivo studies of human scalp hair follicles (HFs) have permitted major advances in hair research, spanning diverse fields such as chronobiology, endocrinology, immunology, metabolism, mitochondrial biology, neurobiology, pharmacology, pigmentation and stem cell biology. Despite thi...
journal_title:Experimental dermatology
pub_type: 杂志文章,评审
doi:10.1111/exd.12836
更新日期:2015-12-01 00:00:00
abstract::The contribution of immune checkpoint receptors in the immunopathogenesis of various autoimmune diseases has been addressed in previous reports. In this study, the expression profile of T-cell immunoglobulin and mucin-domain containing-3 (Tim-3) and programmed cell death-1 (PD-1) checkpoint molecules was investigated...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.13952
更新日期:2019-06-01 00:00:00
abstract:BACKGROUND:The accuracy of diagnosis of skin cancer and especially of early malignant melanoma is most important to reduce its morbidity and mortality. Previous pilot studies using electrical impedance measurements indicate statistically significant accuracies for the detection of skin cancer. OBJECTIVES:The aim of th...
journal_title:Experimental dermatology
pub_type: 杂志文章,多中心研究
doi:10.1111/j.1600-0625.2011.01285.x
更新日期:2011-08-01 00:00:00
abstract::Stratum corneum (SC) exposed to living tissues, induces inflammation characterized by the formation of mixed cell granulomas consisting of infiltrative polymorphonuclear leukocytes (PMNs) and monocytes/macrophages. In this study, to clarify the mechanism for the later monocyte accumulation in SC-induced granulomas, we...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.1998.tb00316.x
更新日期:1998-08-01 00:00:00
abstract::High-mobility group box-1 (HMGB1) has been implicated as a pro-inflammatory cytokine in the pathogenesis of various inflammatory and autoimmune diseases. However, information about HMGB1 in Henoch-Schönlein purpura (HSP) is still unclear. Herein, we investigated the role of HMGB1 in patients with HSP and the pro-infla...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.12422
更新日期:2014-06-01 00:00:00
abstract::We analysed the correlation between the expression of HIF-1α (hypoxia-inducible factor 1 alpha), the nuclear receptors: VDR (vitamin D receptor), RORα (retinoic acid receptor-related orphan receptor alpha), and RORγ and CYP24A1 (cytochrome P450 family 24 subfamily A member 1) and CYP27B1 (cytochrome P450 family 27 sub...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.14002
更新日期:2019-09-01 00:00:00
abstract:OBJECTIVE:To evaluate the long-term efficacy of etanercept for the management of hidradenitis suppurativa. METHODS:Analysis was based on the long-term follow-up (weeks 24-144) of 10 patients enrolled in a prospective open-label phase II study; etanercept was initially administered subcutaneously 50 mg once weekly for ...
journal_title:Experimental dermatology
pub_type: 信件
doi:10.1111/j.1600-0625.2009.00967.x
更新日期:2010-06-01 00:00:00
abstract::Epidermal growth factor receptor (EGFR) gene amplification and protein overexpression are common in several cancers. EGFR status has seldom been studied in cutaneous squamous carcinomas (SCCs), or their precursors, actinic keratoses (AKs). We evaluated the presence of EGFR genomic aberrations and EGFR protein overexpr...
journal_title:Experimental dermatology
pub_type: 信件
doi:10.1111/j.1600-0625.2009.01028.x
更新日期:2010-02-01 00:00:00
abstract::A significant proportion of patients with skin disease do not respond to treatment and adverse drug reactions are a common problem. Genetic factors are important determinants for both drug efficacy and toxicity. The fields of pharmacogenetics and pharmacogenomics examine inter-individual variations in the DNA sequence...
journal_title:Experimental dermatology
pub_type: 杂志文章,评审
doi:10.1111/j.1600-0625.2009.00852.x
更新日期:2009-04-01 00:00:00
abstract:BACKGROUND:In vivo reflectance confocal microscopy (RCM) represents a promising imaging tool that allows a non-invasive examination of skin morphology in real time at nearly histological resolution, showing microanatomical structures and individual cells. OBJECTIVES:The aim of our study was to evaluate the diagnostic ...
journal_title:Experimental dermatology
pub_type: 临床试验,杂志文章
doi:10.1111/j.1600-0625.2008.00822.x
更新日期:2009-06-01 00:00:00
abstract::We have shown previously in psoriasis a very high prevalence of epidermodysplasia verruciforms-associated human papillomavirus 5 (EVHPV5) DNA and antibodies to human papillomavirus 5 (HPV5) virus-like particle (VLP) L1, and we suggested that this benign hyperproliferative disorder could be a reservoir for EVHPVs. Here...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.0906-6705.2003.00125.x
更新日期:2003-12-01 00:00:00
abstract::Pemphigus vulgaris (PV) is a regulatory T cell (Treg)-associated autoimmune disease. Treg cells maintain immunosuppression by expressing the signature transcription factor FOXP3. MicroRNAs (miRNAs) have frequently emerged as regulators in Treg-mediated immunosuppression. We previously found that miR-338-3p was overexp...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.14111
更新日期:2020-05-09 00:00:00
abstract::We previously reported that sphingosine-1-phosphate (S1P) decreases melanin synthesis via extracellular signal-regulated protein kinase (ERK) activation and microphthalmia-associated transcription factor (MITF) degradation. Although FTY720 is an S1P structural analogue, the effects of FTY720 on melanogenesis are not c...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.2010.01148.x
更新日期:2011-03-01 00:00:00
abstract::Human follicle dermal papilla cells (FDPC) are a specialized population of mesenchymal cells located in the skin. They regulate hair follicle (HF) development and growth, and represent a reservoir of multipotent stem cells. Growing evidence supports the hypothesis that HF cycling is associated with vascular remodeling...
journal_title:Experimental dermatology
pub_type: 信件
doi:10.1111/exd.12670
更新日期:2015-05-01 00:00:00
abstract::It has been reported that the abnormal regulation of melanocyte stem cells (McSCs) causes hair greying; however, little is known about the role of McSCs in skin hyperpigmentation such as solar lentigines (SLs). To investigate the involvement of McSCs in SLs, the canonical Wnt signalling pathway that triggers the diffe...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.12496
更新日期:2014-09-01 00:00:00
abstract::Dendritic cells (DCs) show a Janus-like functional behavior. They help us by their orchestration of numerous immune responses to defend our body against invading pathogenic micro-organisms and also induce regulatory T cells to inhibit immune reactions against autoantigens as well as diverse harmless environmental anti...
journal_title:Experimental dermatology
pub_type: 杂志文章,评审
doi:10.1111/j.1600-0625.2008.00823.x
更新日期:2009-03-01 00:00:00
abstract::An ex vivo porcine skin model was utilized to analyse the penetration of 5-aminolevulinic acid (5-ALA) contained in a nanoemulsion-based formulation BF-200 ALA (10% 5-ALA-hydrochloride) versus 16% aminolevulinate methyl ester-hydrochloride in a commercially cream (MAL cream) by fluorescence microscopy of their common ...
journal_title:Experimental dermatology
pub_type: 信件
doi:10.1111/j.1600-0625.2009.01001.x
更新日期:2010-08-01 00:00:00
abstract::Keratinocyte apoptosis mediated by Fas/Fas ligand molecular interactions and subsequent caspase activation is believed to play an important role in the pathogenesis of atopic dermatitis (AD), in particular for the formation of spongiosis. To estimate epidermal caspase activation in normal and AD skin under in vivo con...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.0906-6705.2006.00428.x
更新日期:2006-06-01 00:00:00