Abstract:
:Paulownia tomentosa is a fast-growing tree species with a considerable economic potential because of its value for wood as well as its high biomass production, and elevated stress tolerance. The objective of the present study was to evaluate the development of adventitious buds in leaves obtained from four-week-old shoots of P. tomentosa, in order to identify the cells involved in in vitro adventitious bud development. Leaves (proximal halves with the petiole) from the first node were excised from four-week-old micropropagated shoots, and cultured on Murashige and Skoog medium, supplemented with 3% (w/v) sucrose, 0.6% (w/v) Sigma agar, 22.7 microM thidiazuron (TDZ) and 2.9 microM indole-3-acetic acid for two weeks, explants were then transferred to the same medium with 0.44 microM N6-benzyladenine for another four weeks. Five explants were collected daily during the two first weeks in TDZ treatment. A total of 140 samples were processed. Most of the buds developed indirectly from the callus formed in the petiole stub, and they became visible after eight-ten days of culture, although some buds were also observed in the area of the laminar cut at the level of the veins. The first histological changes could be observed after two-three days of culture, with the dedifferentiation of some subepidermal and inner parenchyma cells, which exhibited a large, prominent nucleus, densely-stained cytoplasm and a high nucleus-to-cell area ratio. Proliferation of these cells gives rise to meristemoid formation after seven-ten days of culture. Organized cell division in meristemoids allows the formation of bud primordia that emerged from the explants surface. The progressive structural differentiation of the apical meristem, leaf primordia, and procambium strands, led to formation of complete buds that were observed in the exterior of the explants after 10-15 days of culture. Direct development of buds from cells in the subepidermic and/or epidermic layers were observed on the adaxial surface of the petiole. This protocol may be a useful tool for the application of genetic transformation techniques, as it enables to determine specific regions in the foliar explants where the meristemoids formation will take place, and therefore to determine which cells should be the object of genetic transformation.
journal_name
Rev Biol Tropjournal_title
Revista de biologia tropicalauthors
San José Mdel C,Cernadas MJ,Corredoira Esubject
Has Abstractpub_date
2014-06-01 00:00:00pages
809-18issue
2eissn
0034-7744issn
2215-2075journal_volume
62pub_type
杂志文章abstract::Turbinella angulata and Busycon perversum are two marine gastropods that support the 90% of conch fisheries in the Gulf of Mexico, nevertheless there is no information available to support a management strategy for both species. The aim of this work was to obtain preliminary fishery information to promote management a...
journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:
更新日期:2013-03-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:10.15517/rbt.v58i1.20023
更新日期:2010-05-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:10.15517/rbt.v56i1.5511
更新日期:2008-03-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:
更新日期:2011-12-01 00:00:00
abstract::Megalopta Smith, 1853, is a Neotropical genus of nocturnal or crepuscular bees. Two subgenera are recognized with most of its nearly 30 species placed in the nominate subgenus. Species of Megalopta s. str. are more commonly collected than species of Noctoraptor Engel et al. 1997, all presumably parasites of Megalopta ...
journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:10.15517/rbt.v58i1.5207
更新日期:2010-03-01 00:00:00
abstract::Bauxite explotation of the Orinoco River in recent years is an important source of heavy metals discharge in the ecosystem, changing the natural biochemical flow of these elements and their concentrations in water, sediment and organisms. Iron and copper concentrations were measured in the fish Plagioscion squamosissi...
journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::The leishmaniasis is a complex disease system, caused by the protozoan parasite Leishmania and transmitted to humans by the vector Lutzomyia spp. Since it is listed as a neglected disease according to the World Health Organization, the aim of this study was to determine the current and future niche of cutaneous and vi...
journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:
更新日期:2016-09-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:
更新日期:2002-06-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:
更新日期:1985-06-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:
更新日期:2012-09-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:
更新日期:2016-12-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:
更新日期:2008-09-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:
更新日期:1984-06-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:
更新日期:2008-09-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:
更新日期:1977-07-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:
更新日期:2004-12-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:
更新日期:2006-12-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:
更新日期:1993-08-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:10.15517/rbt.v56i2.5610
更新日期:2008-06-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:
更新日期:2005-03-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:10.15517/rbt.v60i4.2073
更新日期:2012-12-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:
更新日期:2010-03-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:10.15517/rbt.v64i3.18806
更新日期:2016-09-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:10.15517/rbt.v57i1-2.11292
更新日期:2009-03-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:10.15517/rbt.v61i1.10942
更新日期:2013-03-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:
更新日期:2010-09-01 00:00:00
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journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:
更新日期:2001-07-01 00:00:00
abstract::The queen conch Eustrombus gigas is an important fisheries resource in the Caribbean region. In Cuba Island the studies about this resource are very scarce and particularly in the Southeastern regions of the country. With the aim to get important fishery information about this gastropod, adult Queen Conch density and ...
journal_title:Revista de biologia tropical
pub_type: 杂志文章
doi:10.15517/rbt.v61i2.11165
更新日期:2013-06-01 00:00:00