Concentration of metabolites from Phoma sp. using microfiltration membrane for increasing bioherbicidal activity.

Abstract:

:This study is focused on the concentration of fermented broth from Phoma sp. to increase its herbicidal activity. For this purpose, biomolecules produced by submerged fermentation using Phoma sp. were concentrated by hollow fiber microfiltration membranes. The membrane feed was separated into two streams (retentate and permeate) and the crude broth was concentrated to 10, 30, 50, 70 and 90% (relative to the initial volume). The retentate samples were submitted to bioassays (triplicate) for evaluating their phytotoxic effects on five young leaves of species of Cucumis sativus and also on pre-emergence of weeds as Bidens pilosa and Amaranthus retroflexus. The highest herbicidal activity was 80.7% obtained for a concentration of 30% in the retentate fraction. At this condition, the bioherbicide presented severe damage symptoms on the detached leaves of Cucumis sativus if compared to the crude fermented broth. In the pre-emergence of B. pilosa and A. retroflexus, 100% control was obtained for assays performed in a germination chamber. For greenhouse assays using the substrate, the control rate of A. retroflexus was dependent of concentration of bioherbicide. The promising results achieved in the research with membrane separation process allow us to propose and develop further studies for evaluating this technology in the concentration of other metabolites produced by fermentation which also have bioherbicidal activity.

journal_name

Environ Technol

journal_title

Environmental technology

authors

Todero I,Confortin TC,Soares JF,Brun T,Luft L,Rabuske JE,Kuhn RC,Tres MV,Zabot GL,Mazutti MA

doi

10.1080/09593330.2018.1441330

subject

Has Abstract

pub_date

2019-07-01 00:00:00

pages

2364-2372

issue

18

eissn

0959-3330

issn

1479-487X

journal_volume

40

pub_type

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