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Increased pulmonary RhoA expression in the nitrofen-induced congenital diaphragmatic hernia rat model.

Abstract：

PURPOSE:Persistent pulmonary hypertension remains a major cause of mortality and morbidity in cases of congenital diaphragmatic hernia (CDH). Recently, RhoA/Rho-kinase-mediated vasoconstriction has been reported to be important in the pathogenesis of pulmonary hypertension (PH). Several recent reports have described that fasudil, a potent Rho-kinase inhibitor and vasodilator, could represent a potential therapeutic option for PH. We designed this study to investigate the hypothesis that the expression level of RhoA is increased in the nitrofen-induced CDH rat model. The expression level of Wnt11, an activator of RhoA, was also evaluated. METHODS:Pregnant rats were treated with or without nitrofen on gestational day 9 (D9). Fetuses were sacrificed on D17, D19 and D21 and were divided into control and CDH groups. Quantitative real-time polymerase chain reaction was performed to determine the pulmonary gene expression levels of both Wnt11 and RhoA. An immunofluorescence study was also performed to evaluate the expression and localization of RhoA. RESULTS:The relative mRNA expression levels of pulmonary Wnt11 and RhoA on D21 were significantly increased in the CDH group compared with the control group (p=0.016 and p=0.008, respectively). The immunofluorescence study confirmed the overexpression of RhoA in the pulmonary vessels of CDH rats on D21. CONCLUSIONS:Our results provide evidence that the RhoA/Rho-kinase-mediated pathway is involved in the pathogenesis of PH in the nitrofen-induced CDH rat model. Our data also suggest that the fasudil, a Rho-kinase inhibitor, could represent a therapeutic option for the treatment of PH in CDH.

journal_name

J Pediatr Surg

journal_title

Journal of pediatric surgery

authors

Takayasu H,Masumoto K,Hagiwara K,Sasaki T,Ono K,Jimbo T,Uesugi T,Gotoh C,Urita Y,Shinkai T,Tanaka H

doi

10.1016/j.jpedsurg.2015.02.063

subject

Has Abstract

pub_date

2015-09-01 00:00:00

pages

1467-71

issue

9

eissn

0022-3468

issn

1531-5037

pii

S0022-3468(15)00165-7

journal_volume

50

pub_type

杂志文章

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