Talin Dependent Mechanosensitivity of Cell Focal Adhesions.

abstract：Background:Volumetric tissue-engineered constructs are limited in development due to the dependence on well-formed vascular networks. Scaffold pore size and the mechanical properties of the matrix dictates cell attachment, proliferation and successive tissue morphogenesis. We hypothesize scaffold pore architecture also...

journal_title：Cellular and molecular bioengineering

authors： Chiou G,Jui E,Rhea AC,Gorthi A,Miar S,Acosta FM,Perez C,Suhail Y,Kshitiz,Chen Y,Ong JL,Bizios R,Rathbone C,Guda T

更新日期：2020-08-18 00:00:00

abstract：INTRODUCTION:Intracellular delivery is a key step for many applications in medicine and for investigations into cellular function. This is particularly true for immunotherapy, which often requires controlled delivery of antigen and adjuvants to the cytoplasm of immune cells. Due to the complex responses generated by th...

journal_title：Cellular and molecular bioengineering

authors： Du F,Liu YG,Scott EA

更新日期：2017-10-01 00:00:00

abstract：Introduction:Fluid shear stress (FSS) is the most common stress produced by mastication, speech, or tooth movement. However, how FSS regulates human periodontal ligament (PDL) cell proliferation and migration as well as the underlying mechanism remains unknown. Methods:FSS (6 dyn/cm2) was produced in a flow chamber. C...

journal_title：Cellular and molecular bioengineering

authors： Zheng L,Shi Q,Na J,Liu N,Guo Y,Fan Y

更新日期：2018-08-02 00:00:00

abstract：:Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) offer unprecedented opportunities to study inherited heart conditions in vitro, but are phenotypically immature, limiting their ability to effectively model adult-onset diseases. Cardiomyopathy is becoming the leading cause of death in patients wit...

journal_title：Cellular and molecular bioengineering

authors： Macadangdang J,Guan X,Smith AS,Lucero R,Czerniecki S,Childers MK,Mack DL,Kim DH

更新日期：2015-09-01 00:00:00

abstract：Introduction:Over the last decade, atomic force microscopy (AFM) has played an important role in understanding nanomechanical properties of various cancer cell lines. This study is focused on Lewis lung carcinoma cell tumours as 3D multicellular spheroid (MS). Not much is know about the mechanical properties of the cel...

journal_title：Cellular and molecular bioengineering

authors： Vyas V,Solomon M,D'Souza GGM,Huey BD

更新日期：2019-05-30 00:00:00

abstract：:Chondrocytes and osteoblasts experience multiple stresses in vivo. The optimum mechanical conditions for cell health are not fully understood. This paper describes the optical and microfluidic mechanical manipulation of single suspended cells enabled by the μPIVOT, an integrated micron resolution particle image veloci...

journal_title：Cellular and molecular bioengineering

authors： Nève N,Kohles SS,Winn SR,Tretheway DC

更新日期：2010-09-01 00:00:00

abstract：Introduction:Immune checkpoint inhibitors that boost cytotoxic T cell-based immune responses have emerged as one of the most promising approaches in cancer treatment. However, it is increasingly being realized that T cell activation needs to be rationally combined with molecularly targeted therapeutics for a maximal an...

journal_title：Cellular and molecular bioengineering

authors： Ramesh A,Natarajan SK,Nandi D,Kulkarni A

更新日期：2019-05-21 00:00:00

abstract：:The biomechanical properties of living cells depend on their molecular building blocks, and are important for maintaining structure and function in cells, the extracellular matrix, and tissues. These biomechanical properties and forces also shape and modify the cellular and extracellular structures under stress. While...

journal_title：Cellular and molecular bioengineering

authors： Liang X,Graf BW,Boppart SA

更新日期：2011-06-01 00:00:00

abstract：:Normal cardiomyocytes are highly dependent on the functional expression of ion channels to form action potentials and electrical coupling with other cells. To fully determine the scientific and therapeutic potential of stem cells for cardiovascular-disease treatment, it is necessary to assess comprehensively the regul...

journal_title：Cellular and molecular bioengineering

authors： Ma Z,Liu Q,Liu H,Yang H,Yun JX,Xu M,Eisenberg CA,Borg TK,Markwald R,Gao BZ

更新日期：2012-09-01 00:00:00

abstract：Introduction:The orientation of collagen fibers in native tissues plays an important role in cell signaling and mediates the progression of tumor cells in breast cancer by a contact guidance mechanism. Understanding how migration of epithelial cells is directed by the alignment of collagen fibers requires in vitro assa...

journal_title：Cellular and molecular bioengineering

authors： Mohammed D,Pardon G,Versaevel M,Bruyère C,Alaimo L,Luciano M,Vercruysse E,Pruitt BL,Gabriele S

更新日期：2019-09-25 00:00:00

abstract：:Chlamydia pneumoniae is a common respiratory pathogen that is considered a highly likely risk factor for atherosclerosis. C. pneumoniae is disseminated from the lung into systemic circulation via infected monocytes and lodges at the atherosclerotic sites. During transit, C. pneumoniae-infected monocytes in circulation...

journal_title：Cellular and molecular bioengineering

authors： Evani SJ,Dallo SF,Murthy AK,Ramasubramanian AK

更新日期：2013-09-01 00:00:00

abstract：:The delivery of therapeutics to the central nervous system (CNS) remains a major challenge in part due to the presence of the blood-brain barrier (BBB). Recently, cell-derived vesicles, particularly exosomes, have emerged as an attractive vehicle for targeting drugs to the brain, but whether or how they cross the BBB ...

journal_title：Cellular and molecular bioengineering

authors： Chen CC,Liu L,Ma F,Wong CW,Guo XE,Chacko JV,Farhoodi HP,Zhang SX,Zimak J,Ségaliny A,Riazifar M,Pham V,Digman MA,Pone EJ,Zhao W

更新日期：2016-12-01 00:00:00

abstract：:Many tissue cells exert contractile forces that mechanically couples them to elastic matrices and that influence cell adhesion, cytoskeletal organization, and even cell differentiation. However, strains within the depths of matrices are often unclear and are likely relevant not only to the fact that some matrices such...

journal_title：Cellular and molecular bioengineering

authors： Sen S,Engler AJ,Discher DE

更新日期：2009-03-01 00:00:00

abstract：:Blood flow-associated shear stress causes physiological and pathophysiological biochemical processes in endothelial cells that may be initiated by alterations in plasma membrane lipid domains characterized as liquid-ordered (l(o)), such as rafts or caveolae, or liquid-disordered (l(d)). To test for domain-dependent sh...

journal_title：Cellular and molecular bioengineering

authors： Tabouillot T,Muddana HS,Butler PJ

更新日期：2011-06-01 00:00:00

abstract：:Endometriosis is a chronic disease in which epithelial and stromal cells that resemble the eutopic endometrium are found in ectopic lesions. In order to examine how microenvironmental factors such as extracellular matrix and macrophages influence disease progression, 12Z (an immortalized ectopic epithelial cell line) ...

journal_title：Cellular and molecular bioengineering

authors： Pollock K,Jaraczewski TJ,Carroll MJ,Lebovic DI,Kreeger PK

更新日期：2014-09-01 00:00:00

abstract：:The fluidity of cellular membranes imparts lateral mobility of proteins across the cell surface. To understand the impact of lateral mobility on cell-cell communication, a protein consisting of the extracellular recognition domains of E-cadherin was associated with the surface of silica beads by either tethering to a ...

journal_title：Cellular and molecular bioengineering

authors： Tsai J,Kam LC

更新日期：2010-03-01 00:00:00

abstract：:Primary cilia are sensory organelles that have been shown to play a critical role in lineage commitment. It was our hypothesis that the primary cilium is necessary for chemically induced differentiation of human mesenchymal stem cells (MSC). To investigate this, polaris siRNA was used to inhibit the primary cilia and ...

journal_title：Cellular and molecular bioengineering

authors： Tummala P,Arnsdorf EJ,Jacobs CR

更新日期：2010-09-01 00:00:00

abstract：:In developing implantable tissues based on cellular remodeling of a fibrin scaffold, a key indicator of success is high collagen content. Cellular collagen synthesis is stimulated by cyclic stretching but is limited by cellular adaptation. Adaptation is mediated by deactivation of extracellular signal-regulated kinase...

journal_title：Cellular and molecular bioengineering

authors： Weinbaum JS,Schmidt JB,Tranquillo RT

更新日期：2013-09-01 00:00:00

abstract：Introduction:Neuronal cells are sensitive to mechanical properties of extracellular matrix (ECM) such as stiffness and topography. Cells contract and exert a force on ECM to detect the microenvironment, which activates the signaling pathway to influence the cell functions such as differentiation, migration, and prolife...

journal_title：Cellular and molecular bioengineering

authors： Yasodharababu M,Nair AK

更新日期：2020-05-04 00:00:00

abstract：:The stromal vascular fraction (SVF) of human adipose tissue is a heterogeneous population, with component cell types that may or may not contribute to its regenerative potential. Recent findings indicate that single-cell mechanical biomarkers are characteristic of cell type and can be used comparably to gene and prote...

journal_title：Cellular and molecular bioengineering

authors： Kanthilal M,Darling EM

更新日期：2014-12-01 00:00:00

abstract：:The generation of stable intracellular concentration gradients is a useful method for local control of cell function, selective manipulation of cellular structures and testing hypotheses related to dynamical intracellular processes. Cell culture in a microfluidic device allows the presentation of a stable gradient of ...

journal_title：Cellular and molecular bioengineering

authors： Aggarwal V,Lele TP

更新日期：2016-12-16 00:00:00

abstract：:Actomyosin kinetics in both skinned skeletal muscle fibers at maximum Ca2+-activation and unregulated in vitro motility assays are modulated by solvent microviscosity in a manner consistent with a diffusion limited process. Viscosity might also influence cardiac thin filament Ca2+-regulatory protein dynamics. In vitro...

journal_title：Cellular and molecular bioengineering

authors： Loong CK,Takeda AK,Badr MA,Rogers JS,Chase PB

更新日期：2013-06-01 00:00:00

abstract：Introduction:Controlling gene expression is a fundamental goal of basic and synthetic biology because it allows insight into cellular function and control of cellular activity. We explored the possibility of generating an optogenetic repressor of gene expression in the model organism Saccharomyces cerevisiae by using l...

journal_title：Cellular and molecular bioengineering

authors： Geller SH,Antwi EB,Di Ventura B,McClean MN

更新日期：2019-09-24 00:00:00

abstract：Introduction:Targeted cancer therapeutics have demonstrated more limited clinical efficacy than anticipated, due to both intrinsic and acquired drug resistance. Underlying mechanisms have been largely attributed to genetic changes, but a substantial proportion of resistance observations remain unexplained by genomic pr...

journal_title：Cellular and molecular bioengineering

authors： Claas AM,Atta L,Gordonov S,Meyer AS,Lauffenburger DA

更新日期：2018-01-01 00:00:00

abstract：:Thermoresponsive polymers are being used increasingly in cell culture applications due to their temperature dependent surface properties. Poly(MEO2MA-co-OEGMA) (PMO) brushes offer tunable physical properties via variation in the copolymer ratio, but the effects of composition on cell-substrate interactions is unclear....

journal_title：Cellular and molecular bioengineering

authors： Anderson CR,Abecunas C,Warrener M,Laschewsky A,Wischerhoff E

更新日期：2016-08-24 00:00:00

abstract：Introduction:The mechanical response of large multi-cellular collectives to external stretch has remained largely unexplored, despite its relevance to normal function and to external challenges faced by some tissues. Here, we introduced a simple hybrid silicone substrate to enable external stretch while providing a phy...

journal_title：Cellular and molecular bioengineering

authors： Bashirzadeh Y,Dumbali S,Qian S,Maruthamuthu V

更新日期：2019-02-01 00:00:00

abstract：:In our previous work, bone cell networks with controlled spacing and functional intercellular gap junctions had been successfully established by using microcontact printing and self assembled monolayers technologies [Guo, X. E., E. Takai, X. Jiang, Q. Xu, G. M. Whitesides, J. T. Yardley, C. T. Hung, E. M. Chow, T. Han...

journal_title：Cellular and molecular bioengineering

authors： Huo B,Lu XL,Hung CT,Costa KD,Xu Q,Whitesides GM,Guo XE

更新日期：2008-03-01 00:00:00

abstract：Introduction:Compound D and DMPBD are compounds extracted from Plai or Zingiber cassumunar Roxb., which have antiasthmatic properties. Thai herbal pharmacopoeia have indicated that approximate 50% of Thai prescriptions for asthma contain Plai. However, the inhibition mechanisms of these compounds are not clearly known....

journal_title：Cellular and molecular bioengineering

authors： Jitapunkul K,Poachanukoon O,Hannongbua S,Toochinda P,Lawtrakul L

更新日期：2017-11-17 00:00:00

abstract：:The cell nucleus is becoming increasingly recognized as a mechanosensitive organelle. Most research on nuclear mechanosignaling focuses on the nuclear lamina and coupled actin structures. In this commentary, we discuss the possibility that the nuclear membrane senses and transduces mechanical signals similar to the pl...

journal_title：Cellular and molecular bioengineering

authors： Enyedi B,Niethammer P

更新日期：2016-06-01 00:00:00

abstract：:Recent evidence suggests that the spontaneously hypertensive rat (SHR) has an elevated level of proteases, including matrix metalloproteinases (MMPs), involved in cell membrane receptor cleavage. We hypothesize that SHR red blood cells (RBCs) may be subject to an enhanced glycocalyx cleavage compared to the RBCs of th...

journal_title：Cellular and molecular bioengineering

authors： Pot C,Chen AY,Ha JN,Schmid-Schönbein GW

更新日期：2011-12-01 00:00:00