Proteolytic Cleavage of the Red Blood Cell Glycocalyx in a Genetic Form of Hypertension.

Abstract:

:Recent evidence suggests that the spontaneously hypertensive rat (SHR) has an elevated level of proteases, including matrix metalloproteinases (MMPs), involved in cell membrane receptor cleavage. We hypothesize that SHR red blood cells (RBCs) may be subject to an enhanced glycocalyx cleavage compared to the RBCs of the normotensive Wistar-Kyoto (WKY) rats. By direct observation of RBC rouleaux, we found no significant difference in RBC aggregation for unseparated SHR and WKY RBCs. However, lighter SHR RBCs have a greater tendency to aggregate than WKY RBCs when separated by centrifugation. When SHR plasma was mixed with WKY RBCs, SHR plasma proteases cleaved the glycocalyx of WKY RBCs, a process that can be blocked by MMP inhibition. When treated with MMPs, WKY RBCs showed strong aggregation in dextran but not in fibrinogen, indicating that RBC membrane glycoproteins from the inner core of the glycocalyx were cleaved and that dextran was able to bind to the lipid portion of the RBC membrane. In contrast, treatment with amylases produced fibrinogen-induced aggregation with fibrinogen binding to the protein core. MMP cleavage of RBC glycocalyx reduces RBC adhesion to macrophages as a mechanism to remove old RBCs from the circulation.

journal_name

Cell Mol Bioeng

authors

Pot C,Chen AY,Ha JN,Schmid-Schönbein GW

doi

10.1007/s12195-011-0180-0

subject

Has Abstract

pub_date

2011-12-01 00:00:00

pages

678-692

issue

4

eissn

1865-5025

issn

1865-5033

journal_volume

4

pub_type

杂志文章
  • Nanomechanical Analysis of Extracellular Matrix and Cells in Multicellular Spheroids.

    abstract:Introduction:Over the last decade, atomic force microscopy (AFM) has played an important role in understanding nanomechanical properties of various cancer cell lines. This study is focused on Lewis lung carcinoma cell tumours as 3D multicellular spheroid (MS). Not much is know about the mechanical properties of the cel...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-019-00577-0

    authors: Vyas V,Solomon M,D'Souza GGM,Huey BD

    更新日期:2019-05-30 00:00:00

  • Dual Inhibitors-Loaded Nanotherapeutics that Target Kinase Signaling Pathways Synergize with Immune Checkpoint Inhibitor.

    abstract:Introduction:Immune checkpoint inhibitors that boost cytotoxic T cell-based immune responses have emerged as one of the most promising approaches in cancer treatment. However, it is increasingly being realized that T cell activation needs to be rationally combined with molecularly targeted therapeutics for a maximal an...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-019-00576-1

    authors: Ramesh A,Natarajan SK,Nandi D,Kulkarni A

    更新日期:2019-05-21 00:00:00

  • Platelet-Derived Growth Factor Receptor-α and β are Involved in Fluid Shear Stress Regulated Cell Migration in Human Periodontal Ligament Cells.

    abstract:Introduction:Fluid shear stress (FSS) is the most common stress produced by mastication, speech, or tooth movement. However, how FSS regulates human periodontal ligament (PDL) cell proliferation and migration as well as the underlying mechanism remains unknown. Methods:FSS (6 dyn/cm2) was produced in a flow chamber. C...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-018-0546-7

    authors: Zheng L,Shi Q,Na J,Liu N,Guo Y,Fan Y

    更新日期:2018-08-02 00:00:00

  • Optogenetic Repressors of Gene Expression in Yeasts Using Light-Controlled Nuclear Localization.

    abstract:Introduction:Controlling gene expression is a fundamental goal of basic and synthetic biology because it allows insight into cellular function and control of cellular activity. We explored the possibility of generating an optogenetic repressor of gene expression in the model organism Saccharomyces cerevisiae by using l...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-019-00598-9

    authors: Geller SH,Antwi EB,Di Ventura B,McClean MN

    更新日期:2019-09-24 00:00:00

  • A Chemically Defined Common Medium for Culture of C2C12 Skeletal Muscle and Human Induced Pluripotent Stem Cell Derived Spinal Spheroids.

    abstract:Introduction:Multicellular platforms and linked multi organ on chip devices are powerful tools for drug discovery, and basic mechanistic studies. Often, a critical constraint is defining a culture medium optimal for all cells present in the system. In this study, we focused on the key cells of the neuromuscular junctio...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-020-00624-1

    authors: Besser RR,Bowles AC,Alassaf A,Carbonero D,Maciel R,Saporta M,Agarwal A

    更新日期:2020-06-10 00:00:00

  • Effects of Methacrylate-Based Thermoresponsive Polymer Brush Composition on Fibroblast Adhesion and Morphology.

    abstract::Thermoresponsive polymers are being used increasingly in cell culture applications due to their temperature dependent surface properties. Poly(MEO2MA-co-OEGMA) (PMO) brushes offer tunable physical properties via variation in the copolymer ratio, but the effects of composition on cell-substrate interactions is unclear....

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-016-0464-5

    authors: Anderson CR,Abecunas C,Warrener M,Laschewsky A,Wischerhoff E

    更新日期:2016-08-24 00:00:00

  • Intracellular Concentration Gradients That Mirror External Gradients in Microfluidic Flows: A Computational Analysis.

    abstract::The generation of stable intracellular concentration gradients is a useful method for local control of cell function, selective manipulation of cellular structures and testing hypotheses related to dynamical intracellular processes. Cell culture in a microfluidic device allows the presentation of a stable gradient of ...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-016-0474-3

    authors: Aggarwal V,Lele TP

    更新日期:2016-12-16 00:00:00

  • The effect of RGD peptide on 2D and miniaturized 3D culture of HEPM cells, MSCs, and ADSCs with alginate hydrogel.

    abstract::Advancements in tissue engineering require the development of new technologies to study cell behavior in vitro. This study focuses on stem cell behavior within various miniaturized three-dimensional (3D) culture conditions of alginate biomaterials modified with the Arg-Gly-Asp (RGD) peptide known for its role in cell ...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-016-0428-9

    authors: Dumbleton J,Agarwal P,Huang H,Hogrebe N,Han R,Gooch KJ,He X

    更新日期:2016-06-01 00:00:00

  • In Vivo Multiphoton Microscopy for Investigating Biomechanical Properties of Human Skin.

    abstract::The biomechanical properties of living cells depend on their molecular building blocks, and are important for maintaining structure and function in cells, the extracellular matrix, and tissues. These biomechanical properties and forces also shape and modify the cellular and extracellular structures under stress. While...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-010-0147-6

    authors: Liang X,Graf BW,Boppart SA

    更新日期:2011-06-01 00:00:00

  • Talin Dependent Mechanosensitivity of Cell Focal Adhesions.

    abstract::A fundamental question in mechanobiology is how mechanical stimuli are sensed by mechanosensing proteins and converted into signals that direct cells to adapt to the external environment. A key function of cell adhesion to the extracellular matrix (ECM) is to transduce mechanical forces between cells and their extrace...

    journal_title:Cellular and molecular bioengineering

    pub_type: 评审

    doi:10.1007/s12195-014-0364-5

    authors: Yan J,Yao M,Goult BT,Sheetz MP

    更新日期:2015-01-01 00:00:00

  • Manipulation of Suspended Single Cells by Microfluidics and Optical Tweezers.

    abstract::Chondrocytes and osteoblasts experience multiple stresses in vivo. The optimum mechanical conditions for cell health are not fully understood. This paper describes the optical and microfluidic mechanical manipulation of single suspended cells enabled by the μPIVOT, an integrated micron resolution particle image veloci...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-010-0113-3

    authors: Nève N,Kohles SS,Winn SR,Tretheway DC

    更新日期:2010-09-01 00:00:00

  • Engineered Stochastic Adhesion Between Microbes as a Protection Mechanism Against Environmental Stress.

    abstract:Introduction:Microbes aggregate when they display adhesive proteins on their outer membrane surfaces, which then form bridges between microbes. Aggregation protects the inner microbes from harsh environmental conditions such as high concentrations of antibiotics, high salt conditions, and fluctuations in pH. The protec...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-018-0552-9

    authors: Lewis DD,Vanella R,Vo C,Rose L,Nash M,Tan C

    更新日期:2018-09-06 00:00:00

  • Mechanical response of an epithelial island subject to uniaxial stretch on a hybrid silicone substrate.

    abstract:Introduction:The mechanical response of large multi-cellular collectives to external stretch has remained largely unexplored, despite its relevance to normal function and to external challenges faced by some tissues. Here, we introduced a simple hybrid silicone substrate to enable external stretch while providing a phy...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-018-00560-1

    authors: Bashirzadeh Y,Dumbali S,Qian S,Maruthamuthu V

    更新日期:2019-02-01 00:00:00

  • Matrix strains induced by cells: Computing how far cells can feel.

    abstract::Many tissue cells exert contractile forces that mechanically couples them to elastic matrices and that influence cell adhesion, cytoskeletal organization, and even cell differentiation. However, strains within the depths of matrices are often unclear and are likely relevant not only to the fact that some matrices such...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-009-0052-z

    authors: Sen S,Engler AJ,Discher DE

    更新日期:2009-03-01 00:00:00

  • Lateral Mobility of E-cadherin Enhances Rac1 Response in Epithelial Cells.

    abstract::The fluidity of cellular membranes imparts lateral mobility of proteins across the cell surface. To understand the impact of lateral mobility on cell-cell communication, a protein consisting of the extracellular recognition domains of E-cadherin was associated with the surface of silica beads by either tethering to a ...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-010-0104-4

    authors: Tsai J,Kam LC

    更新日期:2010-03-01 00:00:00

  • How Cells feel their environment: a focus on early dynamic events.

    abstract::It is now well demonstrated that cell adhesion to a foreign surface strongly influences prominent functions such as survival, proliferation, differentiation, migration or mediator release. Thus, a current challenge of major practical and theoretical interest is to understand how cells process and integrate environment...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-008-0009-7

    authors: Cretel E,Pierres A,Benoliel AM,Bongrand P

    更新日期:2008-03-01 00:00:00

  • The Role of Primary Cilia in Mesenchymal Stem Cell Differentiation: A Pivotal Switch in Guiding Lineage Commitment.

    abstract::Primary cilia are sensory organelles that have been shown to play a critical role in lineage commitment. It was our hypothesis that the primary cilium is necessary for chemically induced differentiation of human mesenchymal stem cells (MSC). To investigate this, polaris siRNA was used to inhibit the primary cilia and ...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-010-0127-x

    authors: Tummala P,Arnsdorf EJ,Jacobs CR

    更新日期:2010-09-01 00:00:00

  • Endometriotic Epithelial Cell Response to Macrophage-Secreted Factors is Dependent on Extracellular Matrix Context.

    abstract::Endometriosis is a chronic disease in which epithelial and stromal cells that resemble the eutopic endometrium are found in ectopic lesions. In order to examine how microenvironmental factors such as extracellular matrix and macrophages influence disease progression, 12Z (an immortalized ectopic epithelial cell line) ...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-014-0339-6

    authors: Pollock K,Jaraczewski TJ,Carroll MJ,Lebovic DI,Kreeger PK

    更新日期:2014-09-01 00:00:00

  • Scaffold Architecture and Matrix Strain Modulate Mesenchymal Cell and Microvascular Growth and Development in a Time Dependent Manner.

    abstract:Background:Volumetric tissue-engineered constructs are limited in development due to the dependence on well-formed vascular networks. Scaffold pore size and the mechanical properties of the matrix dictates cell attachment, proliferation and successive tissue morphogenesis. We hypothesize scaffold pore architecture also...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-020-00648-7

    authors: Chiou G,Jui E,Rhea AC,Gorthi A,Miar S,Acosta FM,Perez C,Suhail Y,Kshitiz,Chen Y,Ong JL,Bizios R,Rathbone C,Guda T

    更新日期:2020-08-18 00:00:00

  • Producing Collagen Micro-stripes with Aligned Fibers for Cell Migration Assays.

    abstract:Introduction:The orientation of collagen fibers in native tissues plays an important role in cell signaling and mediates the progression of tumor cells in breast cancer by a contact guidance mechanism. Understanding how migration of epithelial cells is directed by the alignment of collagen fibers requires in vitro assa...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-019-00600-4

    authors: Mohammed D,Pardon G,Versaevel M,Bruyère C,Alaimo L,Luciano M,Vercruysse E,Pruitt BL,Gabriele S

    更新日期:2019-09-25 00:00:00

  • Slowed Dynamics of Thin Filament Regulatory Units Reduces Ca2+-Sensitivity of Cardiac Biomechanical Function.

    abstract::Actomyosin kinetics in both skinned skeletal muscle fibers at maximum Ca2+-activation and unregulated in vitro motility assays are modulated by solvent microviscosity in a manner consistent with a diffusion limited process. Viscosity might also influence cardiac thin filament Ca2+-regulatory protein dynamics. In vitro...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-013-0269-8

    authors: Loong CK,Takeda AK,Badr MA,Rogers JS,Chase PB

    更新日期:2013-06-01 00:00:00

  • Matrix Mechanics Influence Fibroblast-Myofibroblast Transition by Directing the Localization of Histone Deacetylase 4.

    abstract:Introduction:The propagation of mechanochemical signals from the extracellular matrix to the cell nucleus has emerged as a central feature in regulating cellular differentiation and de-differentiation. This process of outside-in signaling and the associated mechanotransduction pathways have been well described in numer...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-017-0493-8

    authors: Li Y,Tang CB,Kilian KA

    更新日期:2017-07-18 00:00:00

  • Interplay Between Cytokine-Induced and Cyclic Equibiaxial Deformation-Induced Nitric Oxide Production and Metalloproteases Expression in Human Alveolar Epithelial Cells.

    abstract::Ventilator-induced lung overdistension has been a growing concern in the management of mechanically ventilated patients. Mechanical ventilation triggers or enhances the net inflammatory and tissue remodeling activities. Although it has been shown that proinflammatory and tissue remodeling factors play important roles ...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-009-0092-4

    authors: Patel H,Kwon S

    更新日期:2009-12-01 00:00:00

  • Endothelial Cell Membrane Sensitivity to Shear Stress is Lipid Domain Dependent.

    abstract::Blood flow-associated shear stress causes physiological and pathophysiological biochemical processes in endothelial cells that may be initiated by alterations in plasma membrane lipid domains characterized as liquid-ordered (l(o)), such as rafts or caveolae, or liquid-disordered (l(d)). To test for domain-dependent sh...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-010-0136-9

    authors: Tabouillot T,Muddana HS,Butler PJ

    更新日期:2011-06-01 00:00:00

  • An Oscillatory Contractile Pole-Force Component Dominates the Traction Forces Exerted by Migrating Amoeboid Cells.

    abstract::We used principal component analysis to dissect the mechanics of chemotaxis of amoeboid cells into a reduced set of dominant components of cellular traction forces and shape changes. The dominant traction force component in wild-type cells accounted for ~40% of the mechanical work performed by these cells, and consist...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-011-0184-9

    authors: Alonso-Latorre B,Del Álamo JC,Meili R,Firtel RA,Lasheras JC

    更新日期:2011-12-01 00:00:00

  • Nanopatterned Human iPSC-based Model of a Dystrophin-Null Cardiomyopathic Phenotype.

    abstract::Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) offer unprecedented opportunities to study inherited heart conditions in vitro, but are phenotypically immature, limiting their ability to effectively model adult-onset diseases. Cardiomyopathy is becoming the leading cause of death in patients wit...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-015-0413-8

    authors: Macadangdang J,Guan X,Smith AS,Lucero R,Czerniecki S,Childers MK,Mack DL,Kim DH

    更新日期:2015-09-01 00:00:00

  • Hypertrophy changes 3D shape of hiPSC-cardiomyocytes: Implications for cellular maturation in regenerative medicine.

    abstract::Advances in the use of human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes for heart regeneration and in vitro disease models demand a greater understanding of how these cells grow and mature in 3-dimensional space. In this study, we developed an analysis methodology of single cardiomyocytes plated on 2...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-016-0462-7

    authors: Rupert CE,Chang HH,Coulombe KL

    更新日期:2017-02-01 00:00:00

  • Immunotheranostic Polymersomes Modularly Assembled from Tetrablock and Diblock Copolymers with Oxidation-Responsive Fluorescence.

    abstract:INTRODUCTION:Intracellular delivery is a key step for many applications in medicine and for investigations into cellular function. This is particularly true for immunotherapy, which often requires controlled delivery of antigen and adjuvants to the cytoplasm of immune cells. Due to the complex responses generated by th...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-017-0486-7

    authors: Du F,Liu YG,Scott EA

    更新日期:2017-10-01 00:00:00

  • Intracellular Flow Cytometric Measurement of Extracellular Matrix Components in Porcine Intervertebral Disc Cells.

    abstract::The objective of this study was to develop and demonstrate the utility of a novel method of evaluating intracellular levels of extracellular matrix (ECM) components in intervertebral disc (IVD) cells using flow cytometry. By using this method, this study discriminated between cell populations in porcine IVD and examin...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-009-0045-y

    authors: Flagler DJ,Huang CY,Yuan TY,Lu Z,Cheung HS,Gu WY

    更新日期:2009-06-01 00:00:00

  • A Multiscale Model to Predict Neuronal Cell Deformation with Varying Extracellular Matrix Stiffness and Topography.

    abstract:Introduction:Neuronal cells are sensitive to mechanical properties of extracellular matrix (ECM) such as stiffness and topography. Cells contract and exert a force on ECM to detect the microenvironment, which activates the signaling pathway to influence the cell functions such as differentiation, migration, and prolife...

    journal_title:Cellular and molecular bioengineering

    pub_type: 杂志文章

    doi:10.1007/s12195-020-00615-2

    authors: Yasodharababu M,Nair AK

    更新日期:2020-05-04 00:00:00