Abstract:
:Thermoresponsive polymers are being used increasingly in cell culture applications due to their temperature dependent surface properties. Poly(MEO2MA-co-OEGMA) (PMO) brushes offer tunable physical properties via variation in the copolymer ratio, but the effects of composition on cell-substrate interactions is unclear. To this end, a series of PMO brushes (0-8% OEGMA) was fabricated and L-929 fibroblast adhesion and morphology was quantified in the presence of serum (FBS) or after functionalization via the adsorption of fibronectin (FN) and vitronectin (VN). Quantification of the adsorption of model proteins, bovine serum albumin and FN, revealed that the extent of adsorption was correlated to the amount MEO2MA content, which represents the more hydrophobic component in PMO brushes. Cells exhibited delayed attachment and spreading on all PMO substrates in the presence of FBS. After 24 h, cell attachment was comparable; however, increased spreading was correlated with increased MEO2MA content. Adsorption of FN significantly increased initial cell attachment to all PMO surfaces after 2 h. This was not observed with VN; however, both FN and VN increased cell spreading/decreased cell circularity for all PMO substrates relative to FBS. Pure MEO2MA brushes with FN exhibited increased cell spreading/decreased cell circularity relative to other PMO substrates after 2 h, and elicited the highest cell density after 24 h. These results demonstrate that increased MEO2MA content in PMO substrates facilitates cell attachment and spreading, which can be further enhanced by adsorbing FN in the absence of other proteins.
journal_name
Cell Mol Bioengjournal_title
Cellular and molecular bioengineeringauthors
Anderson CR,Abecunas C,Warrener M,Laschewsky A,Wischerhoff Edoi
10.1007/s12195-016-0464-5subject
Has Abstractpub_date
2016-08-24 00:00:00pages
75-88issue
1eissn
1865-5025issn
1865-5033pii
464journal_volume
10pub_type
杂志文章abstract::A fundamental question in mechanobiology is how mechanical stimuli are sensed by mechanosensing proteins and converted into signals that direct cells to adapt to the external environment. A key function of cell adhesion to the extracellular matrix (ECM) is to transduce mechanical forces between cells and their extrace...
journal_title:Cellular and molecular bioengineering
pub_type: 评审
doi:10.1007/s12195-014-0364-5
更新日期:2015-01-01 00:00:00
abstract:Introduction:The mechanical response of large multi-cellular collectives to external stretch has remained largely unexplored, despite its relevance to normal function and to external challenges faced by some tissues. Here, we introduced a simple hybrid silicone substrate to enable external stretch while providing a phy...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-018-00560-1
更新日期:2019-02-01 00:00:00
abstract:Introduction:Microbes aggregate when they display adhesive proteins on their outer membrane surfaces, which then form bridges between microbes. Aggregation protects the inner microbes from harsh environmental conditions such as high concentrations of antibiotics, high salt conditions, and fluctuations in pH. The protec...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-018-0552-9
更新日期:2018-09-06 00:00:00
abstract::The delivery of therapeutics to the central nervous system (CNS) remains a major challenge in part due to the presence of the blood-brain barrier (BBB). Recently, cell-derived vesicles, particularly exosomes, have emerged as an attractive vehicle for targeting drugs to the brain, but whether or how they cross the BBB ...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-016-0458-3
更新日期:2016-12-01 00:00:00
abstract:Introduction:Controlling gene expression is a fundamental goal of basic and synthetic biology because it allows insight into cellular function and control of cellular activity. We explored the possibility of generating an optogenetic repressor of gene expression in the model organism Saccharomyces cerevisiae by using l...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-019-00598-9
更新日期:2019-09-24 00:00:00
abstract::Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) offer unprecedented opportunities to study inherited heart conditions in vitro, but are phenotypically immature, limiting their ability to effectively model adult-onset diseases. Cardiomyopathy is becoming the leading cause of death in patients wit...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-015-0413-8
更新日期:2015-09-01 00:00:00
abstract::Advances in the use of human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes for heart regeneration and in vitro disease models demand a greater understanding of how these cells grow and mature in 3-dimensional space. In this study, we developed an analysis methodology of single cardiomyocytes plated on 2...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-016-0462-7
更新日期:2017-02-01 00:00:00
abstract:Introduction:The propagation of mechanochemical signals from the extracellular matrix to the cell nucleus has emerged as a central feature in regulating cellular differentiation and de-differentiation. This process of outside-in signaling and the associated mechanotransduction pathways have been well described in numer...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-017-0493-8
更新日期:2017-07-18 00:00:00
abstract:Introduction:Neuronal cells are sensitive to mechanical properties of extracellular matrix (ECM) such as stiffness and topography. Cells contract and exert a force on ECM to detect the microenvironment, which activates the signaling pathway to influence the cell functions such as differentiation, migration, and prolife...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-020-00615-2
更新日期:2020-05-04 00:00:00
abstract:Introduction:Fluid shear stress (FSS) is the most common stress produced by mastication, speech, or tooth movement. However, how FSS regulates human periodontal ligament (PDL) cell proliferation and migration as well as the underlying mechanism remains unknown. Methods:FSS (6 dyn/cm2) was produced in a flow chamber. C...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-018-0546-7
更新日期:2018-08-02 00:00:00
abstract::It is now well demonstrated that cell adhesion to a foreign surface strongly influences prominent functions such as survival, proliferation, differentiation, migration or mediator release. Thus, a current challenge of major practical and theoretical interest is to understand how cells process and integrate environment...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-008-0009-7
更新日期:2008-03-01 00:00:00
abstract::Recent evidence suggests that the spontaneously hypertensive rat (SHR) has an elevated level of proteases, including matrix metalloproteinases (MMPs), involved in cell membrane receptor cleavage. We hypothesize that SHR red blood cells (RBCs) may be subject to an enhanced glycocalyx cleavage compared to the RBCs of th...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-011-0180-0
更新日期:2011-12-01 00:00:00
abstract::Primary cilia are sensory organelles that have been shown to play a critical role in lineage commitment. It was our hypothesis that the primary cilium is necessary for chemically induced differentiation of human mesenchymal stem cells (MSC). To investigate this, polaris siRNA was used to inhibit the primary cilia and ...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-010-0127-x
更新日期:2010-09-01 00:00:00
abstract:Introduction:Over the last decade, atomic force microscopy (AFM) has played an important role in understanding nanomechanical properties of various cancer cell lines. This study is focused on Lewis lung carcinoma cell tumours as 3D multicellular spheroid (MS). Not much is know about the mechanical properties of the cel...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-019-00577-0
更新日期:2019-05-30 00:00:00
abstract::In developing implantable tissues based on cellular remodeling of a fibrin scaffold, a key indicator of success is high collagen content. Cellular collagen synthesis is stimulated by cyclic stretching but is limited by cellular adaptation. Adaptation is mediated by deactivation of extracellular signal-regulated kinase...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-013-0289-4
更新日期:2013-09-01 00:00:00
abstract:Introduction:Compound D and DMPBD are compounds extracted from Plai or Zingiber cassumunar Roxb., which have antiasthmatic properties. Thai herbal pharmacopoeia have indicated that approximate 50% of Thai prescriptions for asthma contain Plai. However, the inhibition mechanisms of these compounds are not clearly known....
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-017-0515-6
更新日期:2017-11-17 00:00:00
abstract::The stromal vascular fraction (SVF) of human adipose tissue is a heterogeneous population, with component cell types that may or may not contribute to its regenerative potential. Recent findings indicate that single-cell mechanical biomarkers are characteristic of cell type and can be used comparably to gene and prote...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-014-0350-y
更新日期:2014-12-01 00:00:00
abstract::Ventilator-induced lung overdistension has been a growing concern in the management of mechanically ventilated patients. Mechanical ventilation triggers or enhances the net inflammatory and tissue remodeling activities. Although it has been shown that proinflammatory and tissue remodeling factors play important roles ...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-009-0092-4
更新日期:2009-12-01 00:00:00
abstract::Blood flow-associated shear stress causes physiological and pathophysiological biochemical processes in endothelial cells that may be initiated by alterations in plasma membrane lipid domains characterized as liquid-ordered (l(o)), such as rafts or caveolae, or liquid-disordered (l(d)). To test for domain-dependent sh...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-010-0136-9
更新日期:2011-06-01 00:00:00
abstract:Introduction:Multicellular platforms and linked multi organ on chip devices are powerful tools for drug discovery, and basic mechanistic studies. Often, a critical constraint is defining a culture medium optimal for all cells present in the system. In this study, we focused on the key cells of the neuromuscular junctio...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-020-00624-1
更新日期:2020-06-10 00:00:00
abstract::The cell nucleus is becoming increasingly recognized as a mechanosensitive organelle. Most research on nuclear mechanosignaling focuses on the nuclear lamina and coupled actin structures. In this commentary, we discuss the possibility that the nuclear membrane senses and transduces mechanical signals similar to the pl...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-016-0430-2
更新日期:2016-06-01 00:00:00
abstract:Background:Volumetric tissue-engineered constructs are limited in development due to the dependence on well-formed vascular networks. Scaffold pore size and the mechanical properties of the matrix dictates cell attachment, proliferation and successive tissue morphogenesis. We hypothesize scaffold pore architecture also...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-020-00648-7
更新日期:2020-08-18 00:00:00
abstract::We used principal component analysis to dissect the mechanics of chemotaxis of amoeboid cells into a reduced set of dominant components of cellular traction forces and shape changes. The dominant traction force component in wild-type cells accounted for ~40% of the mechanical work performed by these cells, and consist...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-011-0184-9
更新日期:2011-12-01 00:00:00
abstract:Introduction:The orientation of collagen fibers in native tissues plays an important role in cell signaling and mediates the progression of tumor cells in breast cancer by a contact guidance mechanism. Understanding how migration of epithelial cells is directed by the alignment of collagen fibers requires in vitro assa...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-019-00600-4
更新日期:2019-09-25 00:00:00
abstract::The fluidity of cellular membranes imparts lateral mobility of proteins across the cell surface. To understand the impact of lateral mobility on cell-cell communication, a protein consisting of the extracellular recognition domains of E-cadherin was associated with the surface of silica beads by either tethering to a ...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-010-0104-4
更新日期:2010-03-01 00:00:00
abstract::Advancements in tissue engineering require the development of new technologies to study cell behavior in vitro. This study focuses on stem cell behavior within various miniaturized three-dimensional (3D) culture conditions of alginate biomaterials modified with the Arg-Gly-Asp (RGD) peptide known for its role in cell ...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-016-0428-9
更新日期:2016-06-01 00:00:00
abstract::Normal cardiomyocytes are highly dependent on the functional expression of ion channels to form action potentials and electrical coupling with other cells. To fully determine the scientific and therapeutic potential of stem cells for cardiovascular-disease treatment, it is necessary to assess comprehensively the regul...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-012-0240-0
更新日期:2012-09-01 00:00:00
abstract::Chondrocytes and osteoblasts experience multiple stresses in vivo. The optimum mechanical conditions for cell health are not fully understood. This paper describes the optical and microfluidic mechanical manipulation of single suspended cells enabled by the μPIVOT, an integrated micron resolution particle image veloci...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-010-0113-3
更新日期:2010-09-01 00:00:00
abstract::In our previous work, bone cell networks with controlled spacing and functional intercellular gap junctions had been successfully established by using microcontact printing and self assembled monolayers technologies [Guo, X. E., E. Takai, X. Jiang, Q. Xu, G. M. Whitesides, J. T. Yardley, C. T. Hung, E. M. Chow, T. Han...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-008-0011-0
更新日期:2008-03-01 00:00:00
abstract::The biomechanical properties of living cells depend on their molecular building blocks, and are important for maintaining structure and function in cells, the extracellular matrix, and tissues. These biomechanical properties and forces also shape and modify the cellular and extracellular structures under stress. While...
journal_title:Cellular and molecular bioengineering
pub_type: 杂志文章
doi:10.1007/s12195-010-0147-6
更新日期:2011-06-01 00:00:00