当前位置： SCI文献检索 > PHARMACEUTICAL RESEARCH期刊下所有文献 > Electrospun Zein/PCL Fibrous Matrices Release Tetracycline in a Controlled Manner, Killing Staphylococcus aureus Both in Biofilms and Ex Vivo on Pig Skin, and are Compatible with Human Skin Cells.

Electrospun Zein/PCL Fibrous Matrices Release Tetracycline in a Controlled Manner, Killing Staphylococcus aureus Both in Biofilms and Ex Vivo on Pig Skin, and are Compatible with Human Skin Cells.

Abstract：

PURPOSE:To investigate the destruction of clinically-relevant bacteria within biofilms via the sustained release of the antibiotic tetracycline from zein-based electrospun polymeric fibrous matrices and to demonstrate the compatibility of such wound dressing matrices with human skin cells. METHODS:Zein/PCL triple layered fibrous dressings with entrapped tetracycline were electrospun. The successful entrapment of tetracycline in these dressings was validated. The successful release of bioactive tetracycline, the destruction of preformed biofilms, and the viability of fibroblast (FEK4) cells were investigated. RESULTS:The sustained release of tetracycline from these matrices led to the efficient destruction of preformed biofilms from Staphylococcus aureus MRSA252 in vitro, and of MRSA252 and ATCC 25923 bacteria in an ex vivo pig skin model using 1 × 1 cm square matrices containing tetracycline (30 μg). Human FEK4 cells grew normally in the presence of these matrices. CONCLUSIONS:The ability of the zein-based matrices to destroy bacteria within increasingly complex in vitro biofilm models was clearly established. An ex vivo pig skin assay showed that these matrices, with entrapped tetracycline, efficiently kill bacteria and this, combined with their compatibility with a human skin cell line suggest these matrices are well suited for applications in wound healing and infection control.

journal_name

Pharm Res

journal_title

Pharmaceutical research

authors

Alhusein N,Blagbrough IS,Beeton ML,Bolhuis A,De Bank PA

doi

10.1007/s11095-015-1782-3

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

237-46

issue

1

eissn

0724-8741

issn

1573-904X

pii

10.1007/s11095-015-1782-3

journal_volume

33

pub_type

杂志文章

相关文献

PHARMACEUTICAL RESEARCH文献大全
  • Effect of moisture content on compression properties of two dextrose-based directly compressible diluents.

    abstract：:Moisture sorption characteristics and the effect of moisture content on the compression properties of two dextrose-based directly compressible diluents, namely, Emdex (diluent A) and Sweetrex (diluent B) were studied. Both diluents sorbed moisture rapidly at relative humidities greater than 60%. For both the diluents,...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1015889414533

    authors： Shukla AJ,Price JC

    更新日期：1991-03-01 00:00:00

  • Influence of dosage form on the gastroenteropathy of flurbiprofen in the rat: evidence of shift in the toxicity site.

    abstract：PURPOSE:Gastroduodenal and intestinal permeability were compared after single doses of sustained release and regular release flurbiprofen in the rat to assess possible site-specific formulation-dependent toxicity. METHODS:Pharmacokinetics was assessed and gastrointestinal permeability was evaluated using sucrose and 5...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1012134503107

    authors： Davies NM,Jamali F

    更新日期：1997-11-01 00:00:00

  • Coupling of aggregation and immunogenicity in biotherapeutics: T- and B-cell immune epitopes may contain aggregation-prone regions.

    abstract：:Biotherapeutics, including recombinant or plasma-derived human proteins and antibody-based molecules, have emerged as an important class of pharmaceuticals. Aggregation and immunogenicity are among the major bottlenecks during discovery and development of biotherapeutics. Computational tools that can predict aggregati...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-011-0414-9

    authors： Kumar S,Singh SK,Wang X,Rup B,Gill D

    更新日期：2011-05-01 00:00:00

  • Regulation of miR-19 to breast cancer chemoresistance through targeting PTEN.

    abstract：PURPOSE:To explore whether miR-19 is involved in the regulation of multidrug resistance (MDR), one of the main causes of breast cancer mortality, and modulates sensitivity of tumor cells to chemotherapeutic agents. METHODS:We analyzed miRNA expression levels in three MDR cell lines in comparison with their parent cell...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-011-0570-y

    authors： Liang Z,Li Y,Huang K,Wagar N,Shim H

    更新日期：2011-12-01 00:00:00

  • Pegylated nanoparticles from a novel methoxypolyethylene glycol cyanoacrylate-hexadecyl cyanoacrylate amphiphilic copolymer.

    abstract：PURPOSE:The aim of this work was to develop PEGylated poly(alkylcyanoacrylate) nanoparticles from a novel methoxypolyethyleneglycol cyanoacrylate-co-hexadecyl cyanoacrylate copolymer. METHODS:PEGylated and non-PEGylated nanoparticles were formed by nanoprecipitation or by emulsion/solvent evaporation. Nanoparticles si...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1011973625803

    authors： Peracchia MT,Vauthier C,Desmaële D,Gulik A,Dedieu JC,Demoy M,d'Angelo J,Couvreur P

    更新日期：1998-04-01 00:00:00

  • The implantable and biodegradable PHBHHx 3D scaffolds loaded with protein-phospholipid complex for sustained delivery of proteins.

    abstract：PURPOSE:PHBHHx (poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)) is an excellent biomaterial for tissue repair. Here, we aim to develop a PHBHHx-based three-dimensional (3D) scaffold system for sustained delivery of proteins (insulin serves as a model protein). METHODS:The insulin-phospholipid complex (INS-PLC) was prep...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-012-0944-9

    authors： Peng Q,Yang YJ,Zhang T,Wu CY,Yang Q,Sun X,Gong T,Zhang L,Zhang ZR

    更新日期：2013-04-01 00:00:00

  • Scale of health: indices of safety and efficacy in the evolving environment of large biological datasets.

    abstract：:The interdependent relationship between pharmacology and toxicology is fundamental to the concepts of efficacy and safety of both drugs and xenobiotics. The traditional concept of establishing efficacious and tolerated doses to define a 'therapeutic window' appears simplistic in the context of an exponentially increas...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章,评审

    doi：10.1007/s11095-014-1415-2

    authors： Sayes CM,Staats H,Hickey AJ

    更新日期：2014-09-01 00:00:00

  • Delivery of HSP90 Inhibitor Using Water Soluble Polymeric Conjugates with High Drug Payload.

    abstract：PURPOSE:HSP90 (Heat shock protein 90kD) has been validated as a therapeutic target in Castrate Resistant Prostate Cancer. Unfortunately, HSP90 inhibitors suffer from dose-limiting toxicities that hinder their clinical applications. Previously developed polymeric delivery systems for HSP90 inhibitors had either low drug...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-017-2249-5

    authors： Suárez Del Pino JA,Kolhatkar R

    更新日期：2017-12-01 00:00:00

  • Prolonged blood circulation in rats of nanospheres surface-modified with semitelechelic poly[N-(2-hydroxypropyl)methacrylamide].

    abstract：:Semitelechelic poly[N-(2-hydroxypropyl)methacrylamide]s (ST-PHPMA) containing one amino end-group and differing in molecular weight were synthesized by radical polymerization in the presence of 2-aminoethanethiol (AET) as chain transfer agent. These polymers were covalently attached via amide bonds to the surface of n...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1016247206531

    authors： Kamei S,Kopecek J

    更新日期：1995-05-01 00:00:00

  • Site- and stereospecific ocular drug delivery by sequential enzymatic bioactivation.

    abstract：:Intraocular enzymes convert the ketoxime analogues of some beta-adrenergic blockers via a sequential bioactivation process involving hydrolysis to the corresponding ketones followed by reduction to the aryloxyaminoalcohols, which then exert known and predictable physiological and pharmacological effects only at the si...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1015863521513

    authors： Bodor N,Prokai L

    更新日期：1990-07-01 00:00:00

  • Sensitivity of empirical metrics of rate of absorption in bioequivalence studies.

    abstract：PURPOSE:The sensitivity and effectiveness of indirect metrics proposed for the assessment of comparative absorption rates in bioequivalence studies [Cmax, Tmax, partial AUC (AUCp), feathered slope (SLf), intercept metric (I)] were originally tested by assuming first-order absorption. The present study re-evaluates thei...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1007521016985

    authors： Ring A,Tothfalusi L,Endrenyi L,Weiss M

    更新日期：2000-05-01 00:00:00

  • Reversal of signs of induced cotton effects of dicumarol-alpha 1-acid glycoprotein systems by phenothiazine neuroleptics through ternary complexation.

    abstract：:The interaction of dicumarol and phenothiazine neuroleptics binding to alpha 1-acid glycoprotein (AGP) was investigated by circular dichroism (CD) and equilibrium dialysis. The induced CD spectra of the dicumarol-AGP complex were affected differently by the different substituents of the phenothiazine molecule. The sig...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1015880327911

    authors： Miyoshi T,Yamamichi R,Maruyama T,Otagiri M

    更新日期：1992-07-01 00:00:00

  • Compression behavior of orthorhombic paracetamol.

    abstract：PURPOSE:Orthorhombic crystals of paracetamol exhibit good technological properties during compression. The purpose of this study was to investigate the compression behavior of this substance and to compare it to that of monoclinic paracetamol. From the crystal structure, it could be hypothesized that sliding planes are...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1011954800246

    authors： Joiris E,Di Martino P,Berneron C,Guyot-Hermann AM,Guyot JC

    更新日期：1998-07-01 00:00:00

  • Insight into polycation chain length affecting transfection efficiency by O-methyl-free N,N,N-trimethyl chitosans as gene carriers.

    abstract：PURPOSE:The structure-function relationship and mechanism of polycations as gene carriers have attracted considerable research interest in recent years. The present study was to investigate the relationship between polycation chain length and transfection efficiency (RCL-TE), and the corresponding mechanism by O-methyl...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-013-1211-4

    authors： Xu T,Wang S,Shao Z

    更新日期：2014-04-01 00:00:00

  • Local Application of Pyrophosphorylated Simvastatin Prevents Experimental Periodontitis.

    abstract：PURPOSE:Simvastatin (SIM), a HMG-CoA reductase inhibitor widely prescribed for hypercholesterolemia, has been reported to ameliorate inflammation and promote osteogenesis. Its clinical applications on these potential secondary indications, however, have been hampered by its lack of osteotropicity and poor water solubil...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-018-2444-z

    authors： Wang X,Jia Z,Almoshari Y,Lele SM,Reinhardt RA,Wang D

    更新日期：2018-06-25 00:00:00

  • Renin inhibitor: transport mechanism in rat small intestinal brush-border membrane vesicles.

    abstract：:The transport characteristics of the renin inhibitor ((3S,4S)-4-[N-morpholinoacetyl-(1-naphthyl)-L-alanyl-N-methyl-(4-t hiazolyl)-L- alanyl]amino-3-hydroxy-5-cyclohexyl-1-(4-pyridyl)-1-pentanone; CH3-18) in rat small intestinal brush-border membrane vesicles (BBMV) were examined by a rapid filtration technique. The up...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1018900124257

    authors： Hashimoto N,Fujioka T,Toyoda T,Muranushi N,Hirano K

    更新日期：1994-10-01 00:00:00

  • Esterase-like activity of serum albumin: characterization of its structural chemistry using p-nitrophenyl esters as substrates.

    abstract：PURPOSE:To elucidate the catalytic mechanism of the esterase-like activity of serum albumin (SA), the reactivity of SA from six species was investigated using p-nitrophenyl esters as model substrates. METHODS:The effect of pH and the energetic and thermodynamic profiles of SA were determined for all species for p-nitr...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/b:pham.0000016241.84630.06

    authors： Sakurai Y,Ma SF,Watanabe H,Yamaotsu N,Hirono S,Kurono Y,Kragh-Hansen U,Otagiri M

    更新日期：2004-02-01 00:00:00

  • Metabolite formation pharmacokinetics: rate and extent of metabolite formation determined by deconvolution.

    abstract：:A two-step analytic procedure to determine the rate and extent of metabolite production following administration of the parent compound is described. The procedure provides the rate and extent of metabolite production as a function of time by application of the general model independent approach of deconvolution. The ...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1015951426191

    authors： Karol MD,Goodrich S

    更新日期：1988-06-01 00:00:00

  • Targeted polymeric micelle system for delivery of combretastatin A4 to tumor vasculature in vitro.

    abstract：PURPOSE:To develop an efficient tumor vasculature-targeted polymeric micelle delivery system for combretastatin A4 (CA4), a novel antivascular agent. METHODS:CA4-loaded micelles were prepared from poly (ethylene glycol)-b-poly (d, l-lactide) copolymers. RGD peptides that target integrins alphavbeta3 and alphavbeta5, m...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-010-0184-9

    authors： Wang Y,Yang T,Wang X,Wang J,Zhang X,Zhang Q

    更新日期：2010-09-01 00:00:00

  • Biliary excretion of glycyrrhizin in rats: kinetic basis for multiplicity in bile canalicular transport of organic anions.

    abstract：PURPOSE:To examine the presence of multiplicity for the biliary excretion of xenobiotic conjugates, we studied the disposition of glycyrrhizin (GR), which has glucuronide within its molecular structure and has the ability to inhibit the biliary excretion of liquiritigenin (LG) glucuronides. METHODS:GR was administered...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1016033124819

    authors： Shimamura H,Suzuki H,Tagaya O,Horie T,Sugiyama Y

    更新日期：1996-12-01 00:00:00

  • Solubility of small-molecule crystals in polymers: D-mannitol in PVP, indomethacin in PVP/VA, and nifedipine in PVP/VA.

    abstract：OBJECTIVE:Amorphous pharmaceuticals, a viable approach to enhancing bioavailability, must be stable against crystallization. An amorphous drug can be stabilized by dispersing it in a polymer matrix. To implement this approach, it is desirable to know the drug's solubility in the chosen polymer, which defines the maxima...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-008-9784-z

    authors： Tao J,Sun Y,Zhang GG,Yu L

    更新日期：2009-04-01 00:00:00

  • Amplification of butyrylcholinesterase and acetylcholinesterase genes in normal and tumor tissues: putative relationship to organophosphorous poisoning.

    abstract：:Cholinesterases are ubiquitous carboxylesterase type B enzymes capable of hydrolyzing the neurotransmitter acetylcholine which are transiently expressed in multiple germline, embryonic, and tumor cells. The acute poisoning effects of various organophosphorous compounds are generally attributed to their irreversible co...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章,评审

    doi：10.1023/a:1015867021628

    authors： Soreq H,Zakut H

    更新日期：1990-01-01 00:00:00

  • Pharmacokinetics of once-a-month injectable microspheres of leuprolide acetate.

    abstract：:The pharmacokinetic parameters of leuprolide acetate, a potent analogue of LH-RH, were determined in rats and dogs after i.v. and s.c. dosing with leuprolide solution. The effective human dose of once-a-month injectable microspheres of leuprolide was estimated to be about 3.2 to 8.1 mg analogue/month using these param...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1015818504906

    authors： Okada H,Inoue Y,Heya T,Ueno H,Ogawa Y,Toguchi H

    更新日期：1991-06-01 00:00:00

  • Increased erythropoietin elimination in fetal sheep following chronic phlebotomy.

    abstract：PURPOSE:To determine by pharmacokinetic (PK) means the role of erythropoietin-receptor (EPO-R) upregulation in fetuses on the elimination of erythropoietin (EPO). MATERIALS AND METHODS:Six fetal sheep were catheterized at a gestational age of 125-127 days and phlebotomized daily for 6 days. Paired tracer PK studies us...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-007-9295-3

    authors： Freise KJ,Widness JA,Segar JL,Schmidt RL,Veng-Pedersen P

    更新日期：2007-09-01 00:00:00

  • Pharmacokinetics of a hematoregulatory peptide (SK&F107647) in healthy male volunteers and in patients with colorectal or pancreatic adenocarcinoma not amenable to standard therapy.

    abstract：PURPOSE:To describe the pharmacokinetics of SK&F 107647, a synthetic hematoregulatory peptide, in healthy volunteers and in patients with adenocarcinoma. METHODS:SK&F 107647 pharmacokinetics were evaluated in 2 dose-escalation studies. Volunteers received SK&F 107647 as single 15-minute iv infusion doses of 1, 10, 100...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1007512617139

    authors： Zia-Amirhosseini P,Harris RZ,Cowley HC,Valle JW,Citerone DR,Boppana VK,Scarffe H,Watson P,Davis CB

    更新日期：2000-04-01 00:00:00

  • Mechanical properties of dry and wet cellulosic and acrylic films prepared from aqueous colloidal polymer dispersions used in the coating of solid dosage forms.

    abstract：:The mechanical properties of dry and wet polymeric films prepared from various aqueous polymeric dispersions were evaluated by a puncture test. They were studied with respect to type of polymer dispersion [cellulosic: Aquacoat and Surelease; acrylic: Eudragit NE, L, RS, and RL 30 D], plasticizer type (water-soluble or...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1018942127524

    authors： Bodmeier R,Paeratakul O

    更新日期：1994-06-01 00:00:00

  • Curcumin Delivery by Poly(Lactide)-Based Co-Polymeric Micelles: An In Vitro Anticancer Study.

    abstract：PURPOSE:This work describes the synthesis of block co-polymeric micelles, methoxy-poly(ethylene glycol)-poly(D,L-lactide) (mPEG-PLA) to encapsulate Curcumin (CUR), thereby improving the dispersibility and chemical stability of curcumin, prolonging its cellular uptake and enhancing its bioavailability. METHODS:CUR-mPEG...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-015-1830-z

    authors： Kumari P,Swami MO,Nadipalli SK,Myneni S,Ghosh B,Biswas S

    更新日期：2016-04-01 00:00:00

  • A novel phase inversion-based process for the preparation of lipid nanocarriers.

    abstract：PURPOSE:To develop and subsequently evaluate a novel phase inversion-based method used to formulate lipidic nanocapsules. METHODS:Mechanical properties of emulsions prepared by multiinversion phase processes were investigated using a drop tensiometer. Based on the results obtained, a formulation process was developed ...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/a:1016121319668

    authors： Heurtault B,Saulnier P,Pech B,Proust JE,Benoit JP

    更新日期：2002-06-01 00:00:00

  • In vitro gastrointestinal lipolysis of four formulations of piroxicam and cinnarizine with the self emulsifying excipients Labrasol and Gelucire 44/14.

    abstract：PURPOSE:Labrasol and Gelucire 44/14 are defined admixtures of acylglycerols and PEG esters which are substrates for digestive lipases. METHODS:We investigated their in vitro gastrointestinal lipolysis to understand which compounds are, after digestion, responsible for keeping poorly water-soluble drugs in solution. Th...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1007/s11095-009-9906-2

    authors： Fernandez S,Chevrier S,Ritter N,Mahler B,Demarne F,Carrière F,Jannin V

    更新日期：2009-08-01 00:00:00

  • Pulmonary delivery of deslorelin: large-porous PLGA particles and HPbetaCD complexes.

    abstract：PURPOSE:To compare the systemic delivery of deslorelin following intratracheal administration of different deslorelin formulations. The formulations included dry powders of deslorelin, large-porous deslorelin-poly(lactide-co-glycolide) (PLGA) particles, and small conventional deslorelin-PLGA particles. Also, solution f...

    journal_title：Pharmaceutical research

    pub_type： 杂志文章

    doi：10.1023/b:pham.0000032997.96823.88

    authors： Koushik K,Dhanda DS,Cheruvu NP,Kompella UB

    更新日期：2004-07-01 00:00:00