Abstract:
PURPOSE:Ruthenium complex is a potentially theranostic agent for cancer imaging and therapy, however its application is limited due to poor water-solubility and lack of tumor selectivity. To overcome the above drawbacks, pH-sensitive nanocapsule as a novel targeting carrier was designed to deliver ruthenium complex for treating xenograft tumor of mice. METHODS:The core/shell structured nanocapsule with ruthenium complex tris(1,10-phenanthroline) ruthenium(II) complex (3P-Ru) as the core and a pH-sensitive polymeric material poly (2-diisopropylaminoethyl methacrylate)-block poly(2-aminoethyl methacrylate hydrochloride) (PbPS) as the shell was synthesized and characterized. Meanwhile, the nanocapsule was used to investigate cell viability and evaluate tissue distribution as well as preventing tumor growth efficacy in U251 stem cells tumor-bearing mouse model. RESULTS:The nanocapsule had a size of 103.1 ± 11.3 nm, zeta potential of -40 ± 5.3 mV, EE of 76.7 ± 0.9%, LE of 25.4 ± 0.6% and it could control drug release under different pH conditions. The results of cell uptake showed that the fluorescent 3P-Ru loaded in the nanocapsule could be delivered into cells with high efficiency, and then significantly inhibited U251 proliferation in a concentration-dependent manner. After U251 stem cells were transplanted subcutaneously into mice, the 3P-Ru/PbPS nanocapsule (PbPS-Ru-NC) via intravenous administration could concentrate in tumor area and obviously prevent tumor growth. CONCLUSIONS:The pH-sensitive nanocapsule as a antitumor agent carrier was able to effectively deliver 3P-Ru into gliomas cells, and cell growth was significantly inhibited both in vitro and in vivo. Such pH-sensitive nanocapsule for ruthenium complex delivery would have great potential application in tumor theranostics.
journal_name
Pharm Resjournal_title
Pharmaceutical researchauthors
Chen L,Fu C,Deng Y,Wu W,Fu Adoi
10.1007/s11095-016-2021-2subject
Has Abstractpub_date
2016-12-01 00:00:00pages
2989-2998issue
12eissn
0724-8741issn
1573-904Xpii
10.1007/s11095-016-2021-2journal_volume
33pub_type
杂志文章abstract:PURPOSE:The use of "Trojan Horse" nanocarriers for antibiotics to enhance the activity of antibiotics against susceptible and resistant bacteria is investigated. METHODS:Antibiotic carriers (CD-MAN and CD-GLU) are prepared from β-cyclodextrin grafted with sugar molecules (D-mannose and D-glucose, respectively) via azi...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-016-1861-0
更新日期:2016-05-01 00:00:00
abstract:PURPOSE:Changes in drug absorption and first-pass metabolism have been reported throughout the pediatric age range. Our aim is to characterize both intestinal and hepatic CYP3A-mediated metabolism of midazolam in children in order to predict first-pass and systemic metabolism of CYP3A substrates. METHODS:Pharmacokinet...
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doi:10.1007/s11095-018-2458-6
更新日期:2018-07-30 00:00:00
abstract:PURPOSE:Our previous studies showed that the mRNA level of human organic anion transporter (hOAT) 3 in the kidney was correlated with the rate of elimination of an anionic antibiotic cefazolin. However, the correlation coefficient was not so high. In the present study, therefore, we enrolled more patients to examine wh...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-005-8383-5
更新日期:2005-12-01 00:00:00
abstract::Pediatric brain tumors are most common cancers in childhood and among the leading causes of death in children. Chemotherapy has been used as adjuvant (i.e. after) or neoadjuvant (i.e. before) therapy to surgery and radiotherapy for the management of pediatric brain tumors for more than four decades and gained more att...
journal_title:Pharmaceutical research
pub_type: 杂志文章,评审
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abstract:PURPOSE:In this study, modulation of the immune response against diphtheria toxoid (DT) by various adjuvants in transcutaneous immunization (TCI) with microneedle array pretreatment was investigated. METHODS:TCI was performed on BALB/c mice with or without microneedle array pretreatment using DT as a model antigen co-...
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doi:10.1007/s11095-009-9874-6
更新日期:2009-07-01 00:00:00
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journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-009-9943-x
更新日期:2009-10-01 00:00:00
abstract:PURPOSE:Modulating sialylation of therapeutic glycoproteins may be used to influence their clearance and systemic exposure. We studied the effect of low and high sialylated IL4-10 fusion protein (IL4-10 FP) on in vitro and in vivo bioactivity and evaluated the effect of differential sialylation on pharmacokinetic param...
journal_title:Pharmaceutical research
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doi:10.1007/s11095-019-2744-y
更新日期:2019-12-26 00:00:00
abstract::Enoximone and piroximone are cardiotonic agents for use in patients with congestive heart failure. Electroanalytical studies revealed that the dihydroimidazolone functionality was oxidizable and that this property can be analytically useful. The compounds undergo a two-electron, irreversible oxidation in neutral to ac...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1015811830830
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abstract::The interaction between several marker solutes and a polyolefin laminate polymer was studied in several solutions. Solutions studied included mixtures of sodium chloride and dextrose (at concentrations more less typical of i.v. administration solutions) and several actual i.v. products [lactated Ringer's injection, Di...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1015866420836
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abstract::Hyaluronan (HA: hyaluronic acid) is produced in the interstitium and reaches the blood circulation through the lymph. It is rapidly eliminated by means of specific receptors on liver endothelium. The elimination characteristics of intravenously administered HA were studied in 10 conscious sheep at the normal plasma HA...
journal_title:Pharmaceutical research
pub_type: 杂志文章
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更新日期:1989-08-01 00:00:00
abstract::RNA interference (RNAi) strategies include double-stranded RNA (dsRNA), small interfering RNA (siRNA), short hairpin RNA (shRNA), and microRNA (miRNA). As this is a highly specific technique, efforts have been made to utilize RNAi towards potential knock down of disease-causing genes in a targeted fashion. RNAi has th...
journal_title:Pharmaceutical research
pub_type: 杂志文章,评审
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更新日期:2011-12-01 00:00:00
abstract:PURPOSE:VLPs displaying tumor targeting single-chain variable fragments (VLP-rscFvs) which targets tumor-associated glycoprotein-72 (TAG-72) marker protein have a potential for immunotherapy against colon carcinoma tumors. In this study, scFvs anchored on VLPs using glycosylphosphatidylinositol (GPI) were prepared to t...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-014-1316-4
更新日期:2014-08-01 00:00:00
abstract::A fluorescence measurement system and methods of data analysis were developed to measure rapid kinetics of transdermal transport in vitro. Three variations on the technique were demonstrated, where the receptor compartment concentration was determined by: 1) fluorescence measurements of aliquots removed at discrete ti...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1016254013374
更新日期:1995-04-01 00:00:00
abstract:PURPOSE:The relationship between rat intestinal permeability (Papp) of a range of hydrophilic probe molecules and probe geometry was examined. METHODS:Molecules studies included mannitol, the polyethylene glycols (PEGs) 400, 900, and 4000, the dextran conjugated dye Texas Red (MW 3000) and the polysaccharide inulin (M...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1016091915733
更新日期:1996-10-01 00:00:00
abstract:PURPOSE:To evaluate 26 marketed oncology drugs for time-dependent inhibition (TDI) of cytochrome P450 (CYP) enzymes. Evaluate TDI-positive drugs for potential to generate reactive intermediates. Assess clinical drug-drug interaction (DDI) risk using static mechanistic models. METHODS:Human liver microsomes and CYP-spe...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-012-0724-6
更新日期:2012-07-01 00:00:00
abstract:PURPOSE:To create poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs), where a drug-encapsulating NP core is covered with polyethylene glycol (PEG) in a normal condition but exposes a cell-interactive TAT-modified surface in an environment rich in matrix metalloproteinases (MMPs). METHODS:PLGA NPs were modified w...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-013-1039-y
更新日期:2013-08-01 00:00:00
abstract:PURPOSE:Near-infrared reflectance spectroscopy (NIRS) was used to evaluate and quantify the effect of compression force on the NIR spectra of tablets. METHODS:Flat, white tablets with no orientation (scoring, etc.) were manufactured on a Stokes Rotary Tablet Press. NIRS was used to predict tablet hardness on the follo...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1012071904673
更新日期:1997-01-01 00:00:00
abstract:PURPOSE:The aim of this work was to evaluate whether improved topical delivery of finasteride, focussed to the hair follicles of human scalp skin could be achieved with application of short durations of heat and use of specific chemical penetration enhancers. METHODS:Franz cell experiments with human scalp skin were p...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-020-02822-y
更新日期:2020-05-31 00:00:00
abstract::A quantitative analysis of the dependence of dissolution rate on the relative surface area occupied by two non-interacting drug mixtures from co-compressed slabs is described. The results from the experimental dissolution rates of each component from naproxen/phenytoin co-compressed slabs under laminar flow conditions...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1018979419714
更新日期:1994-10-01 00:00:00
abstract:PURPOSE:An in vitro dynamic pharmacokinetic (PK) cell culture system was developed to more precisely simulate physiologic nanoparticle/drug exposure. METHODS:A dynamic PK cell culture system was developed to more closely reflect physiologic nanoparticle/drug concentrations that are changing with time. Macrophages were...
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pub_type: 杂志文章
doi:10.1007/s11095-019-2576-9
更新日期:2019-02-01 00:00:00
abstract::The mechanical properties of dry and wet polymeric films prepared from various aqueous polymeric dispersions were evaluated by a puncture test. They were studied with respect to type of polymer dispersion [cellulosic: Aquacoat and Surelease; acrylic: Eudragit NE, L, RS, and RL 30 D], plasticizer type (water-soluble or...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1018942127524
更新日期:1994-06-01 00:00:00
abstract:PURPOSE:The aim of this study was to investigate the depth-dependent intradermal immunogenicity of inactivated polio vaccine (IPV) delivered by depth-controlled microinjections via hollow microneedles (HMN) and to investigate antibody response enhancing effects of IPV immunization adjuvanted with CpG oligodeoxynucleoti...
journal_title:Pharmaceutical research
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更新日期:2016-09-01 00:00:00
abstract::The delivery of therapeutic nucleic acids to neurons has the potential to treat neurological disease and spinal cord injury. While select viral vectors have shown promise as gene carriers to neurons, their potential as therapeutic agents is limited by their toxicity and immunogenicity, their broad tropism, and the cos...
journal_title:Pharmaceutical research
pub_type: 杂志文章,评审
doi:10.1007/s11095-007-9439-5
更新日期:2008-05-01 00:00:00
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doi:10.1007/s11095-010-0290-8
更新日期:2011-02-01 00:00:00
abstract:PURPOSE:To test the hypothesis of surface displacement as the underlying mechanism for IgG stabilization by polysorbates and HPβCD against surface-induced aggregation. METHODS:Adsorption/desorption-kinetics of IgG-polysorbate 80/-HPβCD were monitored. Maximum bubble pressure method was used for processes within second...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-012-0854-x
更新日期:2013-01-01 00:00:00
abstract:PURPOSE:Modulation of 5-HT3 receptor in the central nervous system (CNS) is a promising approach for treatment of neuropathic pain. The goal was to evaluate the role of P-glycoprotein (Pgp) in limiting exposure of different parts of the CNS to ondansetron (5-HT3 receptor antagonist) using wild-type and genetic knockout...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-020-02929-2
更新日期:2020-09-28 00:00:00
abstract:PURPOSE:The passage of molecules across cell membranes is a crucial step in many physiological processes. We therefore seek physical models of this process, in order to predict permeation for new molecules, and to better understand the important interactions which determine the rate of permeation. METHODS:Several sets...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1007543708522
更新日期:2000-08-01 00:00:00
abstract:PURPOSE:The objective of this investigation is to develop a rational design of Osteotropic Drug Delivery System (ODDS), which we have proposed as a novel method for drug delivery to the skeleton via bisphosphonic prodrug, based on the relationship between physicochemical and pharmacokinetic properties of bisphosphonate...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1011033326980
更新日期:2001-05-01 00:00:00
abstract::Dihydropyridine ⇄ pyridinium salt-based brain-selective delivery systems were synthesized for the progestins, ethisterone, norethindrone, and norgestrel. After initial lipophilicity and in vitro studies indicated the feasibility of applying these compounds to brain-specific delivery, in vivo distribution studies were ...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/A:1016359301852
更新日期:1986-10-01 00:00:00
abstract::Despite substantial research carried out over the last decades, it remains difficult to understand the wide range of pharmacological effects of dopaminergic agents. The dopaminergic system is involved in several neurological disorders, such as Parkinson's disease and schizophrenia. This complex system features multipl...
journal_title:Pharmaceutical research
pub_type: 杂志文章,评审
doi:10.1007/s11095-017-2333-x
更新日期:2018-02-15 00:00:00