Abstract:
PURPOSE:VLPs displaying tumor targeting single-chain variable fragments (VLP-rscFvs) which targets tumor-associated glycoprotein-72 (TAG-72) marker protein have a potential for immunotherapy against colon carcinoma tumors. In this study, scFvs anchored on VLPs using glycosylphosphatidylinositol (GPI) were prepared to target colon carcinoma spheroids in vitro. METHODS:VLPs-rscFvs were produced by co-injecting two types of Bombyx mori nucleopolyhedrovirus (BmNPV) bacmids, encoding RSV-gag and rscFvs cDNA into silkworm larvae. Large unilamellar vesicles (LUVs) of 100 nm in diameter were made using 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and packaged with Sulforhodamine B (SRB). LUV-SRB was used to associate with VLP-rscFvs assisted by GP64 present on VLP-rscFvs to produce VLP-rscFv associated SRB (VLP-rscFvs-SRB) at pH 7.5. RESULTS:The antigenicity of the purified VLPs-rScFvs was confirmed by enzyme-linked immunosorbent assay (ELISA) using TAG-72 as antigen. LUV-SRB made of DOPC was used to associate with 100 μg of VLP-rscFvs to produce VLP-rscFv-SRB. Specific delivery and penetration of SRB up to 100 μm into the spheroids shows the potential of the new model. CONCLUSIONS:The current study demonstrated the display, expression and purification of VLP-rscFvs efficiently. As a test model VLP-rscFv-SRB were prepared which can be used for immunotherapy. rscFvs provide the specificity needed to target tumors and VLPs serve as carrier transporting the dye to target.
journal_name
Pharm Resjournal_title
Pharmaceutical researchauthors
Deo VK,Yui M,Alam J,Yamazaki M,Kato T,Park EYdoi
10.1007/s11095-014-1316-4subject
Has Abstractpub_date
2014-08-01 00:00:00pages
2166-77issue
8eissn
0724-8741issn
1573-904Xjournal_volume
31pub_type
杂志文章abstract:PURPOSE:The investigation was aimed at developing micellar nanocarriers for nose-to-brain delivery of zolmitriptan with the objective to investigate the pathway involved in the drug transport. METHODS:The micellar nanocarrier was successfully formulated and characterized for particle size and shape by multi-angle dyna...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-009-0041-x
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abstract:PURPOSE:To study the impact of different process conditions and formulation compositions on metastable mannitol forms in protein formulations during lyophilization. METHODS:Mannitol was studied with and without other formulation components. A cryostage was used to mimic the different processing steps during lyophiliza...
journal_title:Pharmaceutical research
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doi:10.1007/s11095-012-0855-9
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abstract:PURPOSE:Radial basis function artificial neural networks and theoretical descriptors were used to develop a quantitative structure-pharmacokinetic relationship for structurally diverse drug compounds. METHODS:Human bioavailability values were taken from the literature and descriptors were generated from the drug struc...
journal_title:Pharmaceutical research
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doi:10.1023/b:pham.0000012154.09631.26
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abstract:PURPOSE:The current study aimed to examine the pharmaceutical applications of the focused-ion-beam (FIB) in the inhalation aerosol field, particularly to particle porosity determination (i.e. percentage of particles having a porous interior). MATERIALS AND METHODS:The interior of various spray dried particles (bovine ...
journal_title:Pharmaceutical research
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更新日期:2007-09-01 00:00:00
abstract:PURPOSE:Pulmonary antibiotic delivery is recommended as maintenance therapy for cystic fibrosis (CF) patients who experience chronic infections. However, abnormally thick and sticky mucus present in the respiratory tract of CF patients impairs mucus penetration and limits the efficacy of inhaled antibiotics. To overcom...
journal_title:Pharmaceutical research
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abstract:PURPOSE:A mechanism-based PK-PD model was developed to predict the time course of dopamine D(2) receptor occupancy (D(2)RO) in rat striatum following administration of olanzapine, an atypical antipsychotic drug. METHODS:A population approach was utilized to quantify both the pharmacokinetics and pharmacodynamics of ol...
journal_title:Pharmaceutical research
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journal_title:Pharmaceutical research
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journal_title:Pharmaceutical research
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journal_title:Pharmaceutical research
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doi:10.1007/s11095-016-1887-3
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journal_title:Pharmaceutical research
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更新日期:2013-05-01 00:00:00
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journal_title:Pharmaceutical research
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journal_title:Pharmaceutical research
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journal_title:Pharmaceutical research
pub_type: 杂志文章
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更新日期:2019-08-02 00:00:00
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journal_title:Pharmaceutical research
pub_type: 杂志文章
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更新日期:2007-06-01 00:00:00
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doi:10.1007/s11095-012-0766-9
更新日期:2012-09-01 00:00:00
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journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-011-0636-x
更新日期:2012-04-01 00:00:00
abstract::Moisture sorption characteristics and the effect of moisture content on the compression properties of two dextrose-based directly compressible diluents, namely, Emdex (diluent A) and Sweetrex (diluent B) were studied. Both diluents sorbed moisture rapidly at relative humidities greater than 60%. For both the diluents,...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1015889414533
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abstract:PURPOSE:Chemically modified heparin-DOCA was prepared and found to have markedly lower anticoagulant activity than heparin. In the present study, we elucidated the antiangiogenic and antitumoral activities of heparin-DOCA derivative. METHODS:To evaluate the antiangiogenic and antitumoral effects of heparin-DOCA, capil...
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1007/s11095-006-9139-6
更新日期:2007-01-01 00:00:00
abstract::The absorption of insulin across the vaginal mucosa into the systemic circulation was studied in ovariectomized rats given subsequent estrogen treatment. Blood glucose levels were determined as an indirect measure of insulin absorption, and the effect of various enhancers on the hypoglycemic response was investigated....
journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1015892630637
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更新日期:1996-04-01 00:00:00
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更新日期:2006-11-01 00:00:00
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journal_title:Pharmaceutical research
pub_type: 杂志文章
doi:10.1023/a:1015834114359
更新日期:1991-12-01 00:00:00
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更新日期:2018-08-30 00:00:00
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journal_title:Pharmaceutical research
pub_type: 杂志文章
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更新日期:1991-07-01 00:00:00
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journal_title:Pharmaceutical research
pub_type: 杂志文章
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更新日期:2011-08-01 00:00:00
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journal_title:Pharmaceutical research
pub_type: 杂志文章
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更新日期:1991-10-01 00:00:00
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更新日期:2013-05-01 00:00:00
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journal_title:Pharmaceutical research
pub_type: 杂志文章
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更新日期:2003-05-01 00:00:00