Abstract:
OBJECTIVE:To provide selectable microRNA for intervening diabetes mellitus diseases, NOD mice's expression of microRNA in pancreas tissues and blood under the exendin-4 intervention of was observed and the difference of microRNA target gene was screened. MATERIALS AND METHODS:Forty clean NOD mice were randomly divided into four groups (in each group, n = 10): One is blank control group D which is intervened with normal saline, and the other three groups were divided into low-dose group A, middle-dose group B, and high-dose group C according to the different exendin-4 dosage 2, 4, and 8 μg/kg·d. After the 8-week intervention, these four groups were killed, and the pancreatic tissue and blood were left to prepare specimens for morphology and molecular biology analysis. The specimen with differential expression microRNA in pancreas tissue and blood should be screened out after detected with the locked nucleic acid array system (LNATM) microRNA expression profile chip. The primers should be designed, and the ABI7500 real-time fluorescent quantitative PCR should be applied to amplify, analyze, and verify according to the screen results of the microRNA chip in order to screen out the significant differentially expressed microRNA. RESULTS:Histological detection showed that the pancreas of the mice in control group D was fibrosis gradually and the islet frame was relatively disordered and significantly atrophied. Groups A, B, and C have no islet hypertrophy or atrophy and the degree of fibrosis of the pancreas has reduced. According to the gene chip detection, there are four significantly differently expressed microRNAs in pancreas tissue and blood among the group A, B, and C, among which miR-19a, miR-19b, and miR-22 were downregulated expressed while the miRNA-1 was upregulated expressed. Bioinformatics analysis showed that the target genes of 4 differentially regulated microRNA genes were related to cell proliferation, apoptosis, glucose metabolism, and angiogenesis. The expression of microRNA in pancreatic tissue and blood of NOD rats was highly consistent. CONCLUSIONS:MicroRNA expression file of pancreatic tissue and blood can be changed during the intervention of the NOD rat model with exendin-4. MicroRNA that indicates the differential expression may take part in the recovering process of the NOD pancreatic trauma. At the same time, the administration of exendin-4 can protect NOD mice, reduce its pancreatic tissue fibrosis, and regulate molecular markers of pancreatic cells in size and pancreatic mast cells. This may be one of the main mechanisms of pancreatic injury in diabetes prevention.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
He JS,Lian CW,Fang YL,Wu JZ,Ye XL,Zhu SBsubject
Has Abstractpub_date
2016-10-01 00:00:00pages
4322-4327issue
20eissn
1128-3602issn
2284-0729journal_volume
20pub_type
杂志文章abstract:BACKGROUND:To investigate the efficacy of a long-term treatment with Deflazacort (DFZ), a third generation synthetic glucocorticoid, in patients affected by Retinitis Pigmentosa (RP) complicated by Cystoid Macular Edema (CME). METHODS:A randomized group of 10 RP subjects were selected for this pilot study and treated ...
journal_title:European review for medical and pharmacological sciences
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2002-01-01 00:00:00
abstract:OBJECTIVE:Midazolam and sufentanil are common analgesic and sedative drugs, but the effects and mechanisms of the combination of these two drugs on pancreatitis injury have not been fully elucidated. MATERIALS AND METHODS:Rats pancreatitis model were randomly divided into 4 groups, model group, midazolam group, sufent...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202002_20390
更新日期:2020-02-01 00:00:00
abstract::Sarcoidosis is a granulomatous disease of unknown origin, with pulmonary findings in more than 90% of patients. Extrapulmonary involvement is common and all organs can be involved (especially lymph nodes, eyes, joints, central nervous system) but it is rare to find an isolated extrapulmonary disease (less than 10% of ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2009-03-01 00:00:00
abstract:OBJECTIVE:The outbreak of the 2019 Novel Coronavirus Disease (COVID-19) is seriously threatening the health of people all over China and the world. This study aims to investigate the clinical characteristics and outcomes of COVID-19 patients admitted at different time periods. PATIENTS AND METHODS:A total of 132 disch...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202007_22287
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVE:To explore the effects of lamivudine on cell proliferation of liver cancer and expressions of HBsAg, HBeAg, and MMP-9 in hepatoma cells. MATERIALS AND METHODS:In the intervention group, HepG2.2.15 cells were cultured with lamivudine at 100, 200, and 300 μmol/L for 24 hours, 48 hours, and 72 hours. In the con...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201910_19312
更新日期:2019-10-01 00:00:00
abstract:OBJECTIVE:Osteoarthritis (OA) is one of the most common chronic joint diseases, caused by lesions in articular cartilage and synovial membranes. Synovitis is a major characteristic of OA, due to the proliferation of synoviocytes. Long noncoding RNAs (lncRNAs) have implicated to play an important role in many different ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201808_15705
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVE:The vital role of long noncoding RNAs (lncRNAs) in tumor progression has been identified in numerous studies. Nasopharyngeal carcinoma (NPC) is one of the most ordinary malignant tumors. This study aims to explore the role of lncRNA ROR1-AS1 in the metastasis of NPC. PATIENTS AND METHODS:Real Time-quantitati...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,收录出版
doi:10.26355/eurrev_202001_19909
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVE:To investigate the risk factors related to mortality in severe polytrauma patients with acute hypoxemic respiratory failure (AHRF). PATIENTS AND METHODS:From December 2011 to December 2014, we identified and intubated 524 traumatic AHRF patients in a level 1 trauma centers. Amongst those, we enrolled seventy...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-10-01 00:00:00
abstract::Drug-induced liver diseases (DILD) are clinico-pathologic patterns of liver injury caused by drugs or other foreign compounds. Steatohepatitis is a rare form of DILD, and drugs account for fewer than 2% of non-alcoholic steatohepatitis (NASH). Drugs known to be capable of inducing steatosis and steatohepatitis can be ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2005-09-01 00:00:00
abstract:OBJECTIVE:Some circular RNAs (circRNAs) have been testified to play crucial roles in the regulation of skin melanoma, including circRNA_0016418 (circ0016418). However, the regulatory mechanism of circ0016418 in skin melanoma is undiscovered. MATERIALS AND METHODS:The RNA expression was examined through quantitative Re...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201912_19795
更新日期:2019-12-01 00:00:00
abstract:OBJECTIVE:Prostate cancer (PC) is one of the most ordinary malignant cancers. Recent researches have proved that long noncoding RNAs (lncRNAs) act as an important role in cancers. Our study aims to explore the function of lncRNA CASC15 in the tumor metastasis of PC. PATIENTS AND METHODS:Real Time-quantitative Polymera...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201910_19141
更新日期:2019-10-01 00:00:00
abstract:OBJECTIVE:Previous studies have demonstrated that long non-coding ribonucleic acid (lncRNA) FEZF1-AS1 acts as a cancer-promoting gene. However, no reports have investigated the role of FEZF1-AS1 in oral squamous cell carcinoma (OSCC) yet. Therefore, the aim of this study was to explore whether FEZF1-AS1 promoted the ex...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201908_18534
更新日期:2019-08-01 00:00:00
abstract:OBJECTIVES:Ferula gummosa Boiss is native to central Asia. This plant has traditionally been used in the treatment of many diseases. The antihypoxic and antioxidant activities of Ferula gummosa roots were investigated. MATERIAL AND METHODS:1,1-diphenyl-2-picryl hydrazyl radical (DPPH), nitric oxide and hydrogen peroxi...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2011-06-01 00:00:00
abstract:OBJECTIVES:Control of postoperative symptoms is of paramount importance in proctologic surgery. Phlebotropic activity, protective effect on the capillaries and anti-inflammatory properties of the flavonoids have been reported in several studies. They have been used to treat a variety of conditions including chronic ven...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:With improvement in methods, mortality after duodeno-cefalo pancreatectomy (DCP) has decreased to 5% even if complication rate is still high (30-50%). The pancreatic fistula still occurs in 25-50% of cases. Various methods of treating pancreatic stump have been proposed aimed to improve this rate. PATIENTS A...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2014-12-01 00:00:00
abstract:OBJECTIVE:Oral squamous cell carcinoma (OSCC) is still one of the most frequent neck and head malignancies and is one of the most common cancers in the world. The main purpose of this research was to illustrate the functional role of LINC00961 in OSCC and provide novel insight of biomarkers and therapeutic strategies i...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201904_17699
更新日期:2019-04-01 00:00:00
abstract:OBJECTIVES:Scoliosis is the disease which has a long history over one century. However, the pathogenesis remains unclear at present. To demonstrate the effect of different selenium content in environment on the morbidity of adolescent idiopathic scoliosis (AIS). METHODS:Retrospective cohort study (follow-up from 1997 ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2013-09-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Leishmania parasites are intracellular haemoflagellates that infect macrophages of the skin and viscera to produce diseases in their vertebrates hosts. Antileishmania therapy is based on pentavalent antimony compounds which toxicity of these agents and the persistence of side effects are sever...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2013-12-01 00:00:00
abstract:OBJECTIVE:Prostate cancer is the most frequently diagnosed cancer in men, as well as the second leading cause of death among cancers after lung cancer. In the United States, it is more prevalent in African-American men than in Caucasian men. Prostate cancer frequently metastasizes to the bone, with most of the reported...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-12-01 00:00:00
abstract:OBJECTIVE:The purpose of this study is to examine the feasibility and prospective success associated with implementing and evaluating a six-week live music intervention on an inpatient neurorehabilitation ward. PATIENTS AND METHODS:In total 26 patients were included in this study. Out of which, 15 were patients and 11...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-09-01 00:00:00
abstract:OBJECTIVE:Dynamic pupillometry (DP) is a simple, non-invasive computerized assessment of pupillary light response which provides data concerning both branches of the autonomous nervous system (ANS). Heart rate variability (HRV) analysis assess cardiac health and the ANS modulation on the heart. In this study, we aimed ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-05-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the effect of ulinastatin (UTI) on myocardial ischemia-reperfusion injury (MIRI) through the c-Jun N-terminal kinase (JNK) signaling pathway and p38 mitogen-activated protein kinase (MAPK) signaling pathway. MATERIALS AND METHODS:Healthy adult male Sprague-Dawley (SD)...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201910_19183
更新日期:2019-10-01 00:00:00
abstract:OBJECTIVE:To summarize the latest information on the relationship between genes and common forms of obesity, and to review genetic markers (SNPs and miRNA) that play a role in predisposing to common forms of obesity and related disorders. MATERIALS AND METHODS:We searched PubMed with the following keywords: (obesity[T...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:10.26355/eurrev_201902_17137
更新日期:2019-02-01 00:00:00
abstract:OBJECTIVE:To study the application value of artificial skin substitute compositely constructed by adipose-derived stem cells and fibrin gel in skin defect. MATERIALS AND METHODS:Adipose-derived stem cells (ADSCs) were obtained from healthy pure green fluorescent protein (GFP) transgenic mice and were proved to have mu...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-07-01 00:00:00
abstract:BACKGROUND:Apoptosis involves in testicular germ cell loss in animals and humans, and plays an important role in male fertility. Previous studies have reported neuroprotective and antiapoptotic effects of minocycline. AIM:In this study the protective effect of Minocycline on testicular germ cell apoptosis arising from...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract:OBJECTIVE:To explore the effect of carbachol on myocardial injury in septic rats, and to further study its influence on the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway. MATERIALS AND METHODS:A total of 48 healthy male Sprague-Dawley rats were randomly divided into sham group (n=16), m...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202005_21356
更新日期:2020-05-01 00:00:00
abstract:OBJECTIVE:To study the safety and efficacy of endoscopic mucosal resection (EMR) combined with chemotherapy in patients with early gastric cancer. PATIENTS AND METHODS:Continuously, 112 patients of early gastric cancer (IA-IB) were chosen to receive the treatment of EMR. The 112 patients were divided into two groups, ...
journal_title:European review for medical and pharmacological sciences
pub_type: 临床试验,杂志文章
doi:
更新日期:2016-06-01 00:00:00
abstract:OBJECTIVE:lncRNA MEG3 has been reported as a tumor suppressor gene in many different kinds of cancer, but its role in gastric cancer has not been fully understood. Then, we would like to explore its mechanism in gastric cancer. PATIENTS AND METHODS:We first used qRT-PCR to detect the expression of lncRNA MEG3, then CC...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-10-01 00:00:00
abstract:OBJECTIVE:To investigate the effects of recombinant activated coagulation factor VII (rFVIIa) on apoptosis and the expressions of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax) in rats with intracerebral hemorrhage (ICH). MATERIALS AND METHODS:A total of 90 8-week-old male Sprague-Dawley (SD) rats with...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201808_15725
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVE:To explore the mechanism microRNA-378 in smoking-induced airway inflammation and mucus hypersecretion. MATERIALS AND METHODS:Human bronchial epithelial (HBE) cells were treated with cigarette smoke extract (CSE) to construct the in vitro COPD model. Expression levels of microRNA-378, inflammatory factors and...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201910_19302
更新日期:2019-10-01 00:00:00