Abstract:
:Background: Dried blood spots (DBS) are used for epidemiological surveys on infectious diseases in settings where limited resources are available. In fact, DBS can help to overcome logistic difficulties for the collection, transport and storage of biological specimens. Objective: To evaluate the accuracy of Strongyloides stercoralis serology performed on DBS. Methods: A survey was proposed to children attending a school in the village of Borbon, Ecuador, and to their parents/guardians. Each participant gave consent to the collection of both serum and DBS specimens. DBS absorbed on filter papers were analyzed with a commercially available ELISA test for S. stercoralis antibodies, as well as with standard serology. The agreement between the two methods was assessed through the Cohen's kappa coefficient. Results: The study sample was composed of 174 children and 61 adults, for a total of 235 serum and 235 DBS samples. The serology was positive in 31/235 (13%) serum samples, and in 27/235 (11%) DBS: 4 samples resulted discordant (positive at standard serology). Cohen's kappa coefficient was 0.921 (95% CI 0.845 - 0.998), indicating a high rate of concordance. Conclusion: DBS are suitable for in field-surveys requiring serological testing for S. stercoralis.
journal_name
Front Microbioljournal_title
Frontiers in microbiologyauthors
Formenti F,Buonfrate D,Prandi R,Marquez M,Caicedo C,Rizzi E,Guevara AG,Vicuña Y,Huerlo FR,Perandin F,Bisoffi Z,Anselmi Mdoi
10.3389/fmicb.2016.01778subject
Has Abstractpub_date
2016-11-08 00:00:00pages
1778issn
1664-302Xjournal_volume
7pub_type
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