Abstract:
:Candida albicans biofilm is a main cause of infections associated with medical devices such as catheters, contact lens and artificial joint prosthesis. The current treatment comprises antifungal chemotherapy that presents low success rates. Photodynamic inactivation (PDI) involves the combination of a photosensitizing compound (PS) and light to generate oxidative stress that has demonstrated effective antimicrobial activity against a broad-spectrum of pathogens, including C. albicans. This fungus senses glucose inducing an upregulation of membrane transporters that can facilitate PS uptake into the cell. The aim of this study was to evaluate the effects of glucose on methylene blue (MB) uptake and its influence on PDI efficiency when combined to a red LED with central wavelength at λ=660nm. C. albicans biofilms were grown on hydrogel disks. Prior to PDI assays, MB uptake tests were performed with and without glucose-sensitization. In this system, the optimum PS administration was determined as 500μM of MB in contact with the biofilm during 30min before irradiation. Irradiation was performed during 3, 6, 9, 12, 15 and 18min with irradiance of 127.3mW/cm2. Our results showed that glucose was able to increase MB uptake in C. albicans cells. In addition, PDI without glucose showed a higher viability reduction until 6min; after 9min, glucose group demonstrated a significant decrease in cell viability when compared to glucose-free group. Taken together, our data suggest that glucose is capable to enhance MB uptake and modulate photodynamic inactivation of C. albicans biofilm.
journal_name
Photodiagnosis Photodyn Therjournal_title
Photodiagnosis and photodynamic therapyauthors
Suzuki LC,Kato IT,Prates RA,Sabino CP,Yoshimura TM,Silva TO,Ribeiro MSdoi
10.1016/j.pdpdt.2016.12.003subject
Has Abstractpub_date
2017-03-01 00:00:00pages
173-179eissn
1572-1000issn
1873-1597pii
S1572-1000(16)30122-3journal_volume
17pub_type
杂志文章abstract:AIM:To assess the efficacy of phototherapy on bond strength and microleakage of bulk fill composites in comparison to conventional dentin surface treatments. MATERIALS AND METHODS:Ninety human third molars were divided into 3 groups (n = 30 each) according to surface conditioning treatment. Group 1 (Etch and Bond) EB,...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2018.12.014
更新日期:2019-03-01 00:00:00
abstract::Hidradenitis suppurativa (HS) is a chronic, inflammatory, and recurrent skin disease. Surgical excision of wounds appears to be the only curative treatment for the prevention of recurrence of moderate to severe stages. Magnetic resonance imaging (MRI) is a standard reference examination for the detection of HS peri-an...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2018.05.007
更新日期:2018-09-01 00:00:00
abstract:BACKGROUND:Photodynamic therapy is a promising invention to treat infections and cancer where conventional treatments are insufficient and have many side effects. Photodynamic therapy is mainly emphasized as having minimal side effects on healthy cells during local applications, even so photosensitizer can accumulate i...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2020.101891
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVES:The purpose of this study was to determine the usefulness of a quantitative light-induced fluorescence (QLF) technology in detecting dental restorations by comparing the detection ability of dental hygiene students between using conventional visual inspection alone and visual inspection combined with QLF tec...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.pdpdt.2016.12.010
更新日期:2017-03-01 00:00:00
abstract:AIMS:To evaluate the clinical periodontal, serum glycated haemoglobin (HbA1c) and levels of advanced glycation end-products (AGEs) in the gingival crevicular fluid (GCF) among patients with periodontitis and type 2 diabetes mellitus (DM) after photodynamic therapy (PDT) as an adjunct to full-mouth disinfection (FMD). ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.pdpdt.2019.08.003
更新日期:2019-12-01 00:00:00
abstract:BACKGROUND:Nanosecond pulsed electric field (nsPEF) treatment is a new anti-cancer strategy with ultrashort pulse duration and high intensity of electric fields. The application of nsPEFs affects all intra- and extracellular membranes and independently initiates the process of apoptosis within cancer cells, leading the...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2015.11.002
更新日期:2015-12-01 00:00:00
abstract::A phenaxazone compound [5H-Benzo[a]phenoxazin-5-one (BP)] along with an aminoquinone[2-[(o-hydroxyphenyl)amino]-1,4-naphthaquinone (HAN)] derivatives were synthesized from lawsone using ultrasound irradiation technique. The structure of the compounds were characterized by elemental analysis and various spectral studie...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2017.01.180
更新日期:2017-03-01 00:00:00
abstract:BACKGROUND:Superluminescent diodes (sLED) appear to be an innovative and promising light source in photodynamic therapy (PDT), especially in actinic keratosis (AK) lesions treatment. AIM:Assessment of tolerability and efficacy of sLED in topical 5-aminolevulinic acid (ALA) PDT of AK lesions. METHODS:27 patients recei...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2018.04.010
更新日期:2018-06-01 00:00:00
abstract::Extramedullary plasmacytomas (EMP) are rare plasma cell neoplasms that are mostly located in the mucosal surfaces of the upper aerodigestive tract. The standard treatment is radiotherapy (RT), with surgical resection reserved as salvage procedure. In this report a patient with a EMP, located in the nasopharynx and ref...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2011.03.340
更新日期:2011-09-01 00:00:00
abstract::Chronic renal failure (CRF) is a symptom of kidney damage in the terminal stages. If a patient is not treated, then CRF will progress to uremia, which greatly reduces the lifespan of the patient. However, current screening strategies, including routine urine tests and medical imaging investigations, have poor sensitiv...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2020.101792
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVES:The purpose of this study was to investigate the effects of 5-aminolaevulinic acid mediated photodynamic therapy (ALA-PDT) on the survival activity and apoptosis of human melanoma cell line A375 and non-melanoma skin carcinoma cell line A431 cells. The mechanism for cellular apoptosis was explored. METHODS:...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2018.01.004
更新日期:2018-03-01 00:00:00
abstract:BACKGROUND:Photodynamic diagnosis (PDD) as well as thermovision belong to the category of non-invasive optical diagnosis techniques. Among many different skin cancer diseases, basal cell carcinoma (BCC) is the most frequently occurring one (almost 95% of all skin tumours). In contrast, seborrhoeic keratosis represents ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2012.07.001
更新日期:2013-02-01 00:00:00
abstract::Hidradenitis Suppurativa (HS) is a distressing painful chronic inflammatory skin follicular condition. It is a challenge to achieve better therapeutic effect and lower recurrence rate. In this report, ALA-PDT combined with surgery for the treatment achieved ulcer healing, pain elimination, with no relapse during our f...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2019.07.024
更新日期:2019-12-01 00:00:00
abstract:BACKGROUND:Recently, 5-aminolevulinic acid-based photodynamic therapy (ALA-PDT) is being widely used in cancer therapy owing to the tumor-specific accumulation of photosensitizing protoporphyrin IX (PpIX) after the administration of ALA. In the present study, by focusing on genes involved in the porphyrin biosynthesis ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2011.12.004
更新日期:2012-09-01 00:00:00
abstract::In Endodontics, photosensitizers' such as methylene blue and toluidine blue have been used in Photodynamic Therapy due to their positive results. However, they can stain the dentin from the root canal after Photodynamic Therapy (PDT). The present in vitro study aimed to evaluate different stain removal protocols from ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2017.10.024
更新日期:2017-12-01 00:00:00
abstract:PURPOSE:To investigate the autofluorescence (AF) characteristics of punctate inner choroidopathy (PIC) and to evaluate the progression rate of retinal pigment epithelium (RPE) atrophy lesions in PIC using confocal scanning laser ophthalmoscopy. METHODS:Twenty-two eyes of 14 PIC cases and 21 eyes of 21 non-proliferativ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2014.07.002
更新日期:2014-12-01 00:00:00
abstract::Follicular mucinosis (FM) is a rare inflammatory disorder histologically characterized by mucin deposition in the follicular epithelium. There is no standard therapy for FM and several treatments have been described in the literature. We present the case of a 59 year-old female affected by a recalcitrant FM with diffu...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2019.07.003
更新日期:2019-09-01 00:00:00
abstract:INTRODUCTION/AIM:Photodynamic therapy (PDT) involves the use of a tumor-specific photosensitizer and laser irradiation, and is one of the treatment options recommended for early centrally located lung cancers, but not yet for peripheral-type lung cancers. We developed a new laser probe, the composite-type optical fiber...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2020.101698
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND:Photodynamic therapy (PDT) is a highly efficient approach for tumour therapy, though it also has its drawbacks, too. There are multiple mechanisms involved in cell death regulation that can be successfully targeted for improvement of PDT in particular cases. We assumed, however, that the potential to manage ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2013.04.003
更新日期:2013-12-01 00:00:00
abstract:INTRODUCTION:Clinical studies have shown the usefulness of antimicrobial photodynamic therapy (aPDT) as an adjunctive in periodontal therapy. These studies did not utilize indocyanine green (ICG) as a recently introduced photosensitizer. The aim of this study was to perform a full-mouth double-blind randomized controll...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/j.pdpdt.2016.02.007
更新日期:2016-06-01 00:00:00
abstract:PURPOSE:Early diagnosis of tooth enamel demineralization, and dentin caries lesions, present a valuable solution to avoid or decrease their deleterious effect. The aim of this study was to design a simple, effective, and non-invasive technique, employing a novel laser wavelength to classify and differentiate between va...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2018.11.017
更新日期:2019-03-01 00:00:00
abstract:BACKGROUND:Bacterial resistance is a problem during the process of healing of infected wounds. As a therapy, photodynamic therapy (PDT) has broad-spectral antibacterial activity and non-selective action, which makes it possible to deal with antibiotic resistance.. Methylene blue is a commonly used medicine, but it is r...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2020.102051
更新日期:2020-12-01 00:00:00
abstract:BACKGROUND:Photodynamic therapy has apart from a direct cytotoxic effect also immunomodulatory properties. The aim of our study was to investigate how photodynamic therapy with 5-aminolevulinic acid (ALA-PDT) in sublethal doses influences the secretion of interleukins 6, 8 and 10 from colon cancer cells in vitro. METH...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2018.02.021
更新日期:2018-06-01 00:00:00
abstract:BACKGROUND:Bowenoid papulosis is a polymorphic papular disease that occurs on the external genital area. We investigated the efficacy of 5-aminolevulinic acid-mediated photodynamic therapyin the treatment of Bowenoid papulosis. METHODS:We investigated 200 Bowenoid papulosis cases from the Department of Dermatology and...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2019.101635
更新日期:2020-03-01 00:00:00
abstract::A 35-year-old woman with a history of pulp canal obliteration (PCO) and apical periodontitis was treated with guided endodontics combined with antimicrobial photodynamic therapy (aPDT). The use of aPDT was paramount to the approach performed, and its application should be encouraged in cases of severely calcified cana...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2020.101935
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND:Microorganisms treated by photo-activated disinfection (PAD) in combined periodontal-endodontic (perio-endo) lesions would be exposed to sub-lethal doses of PAD (sPAD). This study evaluated the effect of sPAD using toluidine blue O (TBO) in combination with diode laser irradiation on the growth and biofilm-f...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2016.08.013
更新日期:2016-12-01 00:00:00
abstract::Head and neck (H&N) cancer patients have a high incidence of local field change as well as second primary lung tumours. We have applied the Wolf Diagnostic Autofluorescence Endoscopy (DAFE) system in a novel way, combining autofluorescence evaluation of both H&N region and bronchial. New H&N cases as well as "old" cas...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2006.07.004
更新日期:2006-12-01 00:00:00
abstract:BACKGROUND:Autofluorescence bronchoscopy (AFB) is a highly sensitive tool for the detection of early bronchial cancers. However, its specificity remains limited due to primarily false positive results induced by hyperplasia, metaplasia and inflammation. We have investigated the potential of blue-violet backscattered li...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2008.01.005
更新日期:2008-03-01 00:00:00
abstract:BACKGROUND AND PURPOSE:Breast cancer is one of the most widely recognized tumors. .Diagnosis made in the early stage of disease may imporve outcomes. The discovery of malignant growth utilizing noninvasive light intrusive methods in lieu of conventional excisional biopsy may assist in achieving this goal. MATERIALS AN...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2020.101922
更新日期:2020-09-01 00:00:00
abstract::The success of PDT and its establishment into the existent hall of therapeutic modalities depends on the collection of reported experiences from around the world. In that sense, it is important to report approaches taken by different countries and what their views are on the future of PDT. Following this idea, we pres...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/S1572-1000(05)00058-X
更新日期:2005-06-01 00:00:00