Correlation of rpoB Mutations with Minimal Inhibitory Concentration of Rifampin and Rifabutin in Mycobacterium tuberculosis in an HIV/AIDS Endemic Setting, South Africa.

Abstract:

:Treatment of tuberculosis (TB) and HIV co-infections is often complicated by drug-to-drug interactions between anti-mycobacterial and anti-retroviral agents. Rifabutin (RFB) is an alternative to rifampin (RIF) for TB regimens and is recommended for HIV patients concurrently receiving protease inhibitors because of reduced induction of CYP3A4. This study sought to determine the proportion of RFB susceptible isolates among RIF-resistant strains in a high HIV prevalence setting in South Africa. In addition, the study explored the association between rpoB mutations and minimum inhibitory concentrations (MIC) of RIF and RFB. A total of 189 multidrug resistant (MDR) Mycobacterium tuberculosis isolates from the Centre for Tuberculosis repository were analyzed. The MICs were determined using a MYCOTB Sensititre plate method and the rpoB gene was sequenced. Of the 189 MDR isolates, 138 (73%) showed resistance to both RIF and RFB, while 51 (27%) isolates were resistant to RIF but retained susceptibility to RFB. The S531L was the most frequent rpoB point mutation in 105/189 (56%) isolates, followed by H526Y in 27/189 (14%) isolates. Resistance to both RIF and RFB was found predominantly in association with mutations S531L (91/105, 87%), H526Y (20/27, 74%), and H526D (15/19, 79%), while D516V (15/17, 88%), and L533P (3/4, 75%) were found in RIF-resistant, RFB-susceptible isolates. This study has shown that up to 27% of MDR-TB patients in South Africa may benefit from a treatment regimen that includes RFB.

journal_name

Front Microbiol

authors

Rukasha I,Said HM,Omar SV,Koornhof H,Dreyer AW,Musekiwa A,Moultrie H,Hoosen AA,Kaplan G,Fallows D,Ismail N

doi

10.3389/fmicb.2016.01947

subject

Has Abstract

pub_date

2016-12-05 00:00:00

pages

1947

issn

1664-302X

journal_volume

7

pub_type

杂志文章
  • Sulfate-Reducing Bacteria as an Effective Tool for Sustainable Acid Mine Bioremediation.

    abstract::Mining industries produce vast waste streams that pose severe environmental pollution challenge. Conventional techniques of treatment are usually inefficient and unsustainable. Biological technique employing the use of microorganisms is a competitive alternative to treat mine wastes and recover toxic heavy metals. Mic...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.01986

    authors: Ayangbenro AS,Olanrewaju OS,Babalola OO

    更新日期:2018-08-22 00:00:00

  • Evidence That Microorganisms at the Animal-Water Interface Drive Sea Star Wasting Disease.

    abstract::Sea star wasting (SSW) disease describes a condition affecting asteroids that resulted in significant Northeastern Pacific population decline following a mass mortality event in 2013. The etiology of SSW is unresolved. We hypothesized that SSW is a sequela of microbial organic matter remineralization near respiratory ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.610009

    authors: Aquino CA,Besemer RM,DeRito CM,Kocian J,Porter IR,Raimondi PT,Rede JE,Schiebelhut LM,Sparks JP,Wares JP,Hewson I

    更新日期:2021-01-06 00:00:00

  • Fengycins From Bacillus amyloliquefaciens MEP218 Exhibit Antibacterial Activity by Producing Alterations on the Cell Surface of the Pathogens Xanthomonas axonopodis pv. vesicatoria and Pseudomonas aeruginosa PA01.

    abstract::Bacillus amyloliquefaciens MEP218 is an autochthonous bacterial isolate with antibacterial and antifungal activities against a wide range of phytopathogenic microorganisms. Cyclic lipopeptides (CLP), particularly fengycins, produced by this bacterium; are the main antimicrobial compounds responsible for the growth inh...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.03107

    authors: Medeot DB,Fernandez M,Morales GM,Jofré E

    更新日期:2020-01-21 00:00:00

  • Microbiota in the Rhizosphere and Seed of Rice From China, With Reference to Their Transmission and Biogeography.

    abstract::Seeds play key roles in the acquisition of plant pioneer microbiota, including the transmission of microbes from parent plants to offspring. However, the issues about seed microbial communities are mostly unknown, especially for their potential origins and the factors influencing the structure and composition. In this...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00995

    authors: Zhou X,Wang JT,Zhang ZF,Li W,Chen W,Cai L

    更新日期:2020-07-10 00:00:00

  • Seed and Root Endophytic Fungi in a Range Expanding and a Related Plant Species.

    abstract::Climate change is accelerating the spread of plants and their associated species to new ranges. The differences in range shift capacity of the various types of species may disrupt long-term co-evolved relationships especially those belowground, however, this may be less so for seed-borne endophytic microbes. We collec...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01645

    authors: Geisen S,Kostenko O,Cnossen MC,Ten Hooven FC,Vreš B,van der Putten WH

    更新日期:2017-08-29 00:00:00

  • Deficiency in Lipoteichoic Acid Synthesis Causes a Failure in Executing the Colony Developmental Program in Bacillus subtilis.

    abstract::Colonies are an abundant form of bacterial multicellularity; however, relatively little is known about the initial stages of their construction. We have previously described that colony development of the soil bacterium Bacillus subtilis is a highly ordered process, typically initiating with the formation of extending...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01991

    authors: Mamou G,Fiyaksel O,Sinai L,Ben-Yehuda S

    更新日期:2017-10-24 00:00:00

  • Nutrient Dependent Cross-Kingdom Interactions: Fungi and Bacteria From an Oligotrophic Desert Oasis.

    abstract::Microbial interactions play a key role in ecosystem functioning, with nutrient availability as an important determinant. Although phylogenetically distant bacteria and fungi commonly co-occur in nature, information on their cross-kingdom interactions under unstable, extreme environments remains poor. Hence, the aims o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01755

    authors: Velez P,Espinosa-Asuar L,Figueroa M,Gasca-Pineda J,Aguirre-von-Wobeser E,Eguiarte LE,Hernandez-Monroy A,Souza V

    更新日期:2018-08-06 00:00:00

  • Study of the cwaRS-ldcA Operon Coding a Two-Component System and a Putative L,D-Carboxypeptidase in Lactobacillus paracasei.

    abstract::The cell surface is the primary recognition site between the bacterium and the host. An operon of three genes, LSEI_0219 (cwaR), LSEI_0220 (cwaS), and LSEI_0221 (ldcA), has been previously identified as required for the establishment of Lactobacillus paracasei in the gut. The genes cwaR and cwaS encode a predicted two...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00156

    authors: Scornec H,Palud A,Pédron T,Wheeler R,Petitgonnet C,Boneca IG,Cavin JF,Sansonetti PJ,Licandro H

    更新日期:2020-03-03 00:00:00

  • A First Plasmodium vivax Natural Infection Induces Increased Activity of the Interferon Gamma-Driven Tryptophan Catabolism Pathway.

    abstract::The human immune response that controls Plasmodium infection in the liver and blood stages of the parasite life cycle is composed by both pro- and anti-inflammatory programs. Pro-inflammatory responses primarily mediated by IFN-γ controls the infection, but also induce tolerogenic mechanisms to limit host damage, incl...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00400

    authors: Dos Santos RO,da Cruz MGS,Lopes SCP,Oliveira LB,Nogueira PA,Lima ES,Soares IS,Kano FS,de Carvalho AT,Costa FTM,Ganoza CA,de Lacerda MVG,Lalwani P

    更新日期:2020-03-17 00:00:00

  • Marine sediments microbes capable of electrode oxidation as a surrogate for lithotrophic insoluble substrate metabolism.

    abstract::Little is known about the importance and/or mechanisms of biological mineral oxidation in sediments, partially due to the difficulties associated with culturing mineral-oxidizing microbes. We demonstrate that electrochemical enrichment is a feasible approach for isolation of microbes capable of gaining electrons from ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00784

    authors: Rowe AR,Chellamuthu P,Lam B,Okamoto A,Nealson KH

    更新日期:2015-01-14 00:00:00

  • Response of Tomato Rhizosphere Bacteria to Root-Knot Nematodes, Fenamiphos and Sampling Time Shows Differential Effects on Low Level Taxa.

    abstract::A factorial taxonomic metabarcoding study was carried out to determine the effect of root-knot nematodes (Meloidogyne incognita, RKN) and the nematocide fenamiphos on the rhizosphere microbiome of tomato. Plants inoculated (or not) with RKN second-stage juveniles (J2), and treated (or not) with the nematocide, were te...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00390

    authors: Colagiero M,Rosso LC,Catalano D,Schena L,Ciancio A

    更新日期:2020-03-20 00:00:00

  • Biology and Interaction of the Natural Occurrence of Distinct Monopartite Begomoviruses Associated With Satellites in Capsicum annum From India.

    abstract::Chili (Capsicum annuum L.) is an important vegetable and spice crop of tropical and sub-tropical regions. Chili plants showing upward leaf curling, leaf crinkling, and leaf yellowing symptoms, collected from Sikar district of Rajasthan, India, were found to be associated with begomovirus and satellite molecules. The p...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.512957

    authors: Mishra M,Verma RK,Marwal A,Sharma P,Gaur RK

    更新日期:2020-10-07 00:00:00

  • Chloroplast symbiosis in a marine ciliate: ecophysiology and the risks and rewards of hosting foreign organelles.

    abstract::Simultaneous use of both heterotrophic and autotrophic metabolism ("mixotrophy") is common among protists. Strombidium rassoulzadegani is a planktonic mixotrophic marine ciliate that saves chloroplasts from its algal food and obtains a nutritional subsidy via photosynthesis. Cultures from the northeast, northwest, and...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00321

    authors: McManus GB,Schoener DM,Haberlandt K

    更新日期:2012-09-04 00:00:00

  • The Russian Doll Model: How Bacteria Shape Successful and Sustainable Inter-Kingdom Relationships.

    abstract::Successful inter-kingdom relationships are based upon a dynamic balance between defense and cooperation. A certain degree of competition is necessary to guarantee life spread and development. On the other hand, cooperation is a powerful tool to ensure a long lasting adaptation to changing environmental conditions and ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.573759

    authors: Pessione E

    更新日期:2020-10-20 00:00:00

  • The in vitro Effect of Fibers With Different Degrees of Polymerization on Human Gut Bacteria.

    abstract::Human gut bacteria contribute significantly to human health and several studies have evaluated the effects of dietary fibers on human gut bacterial ecology. However, the relationship between different degrees of fiber polymerization and human gut bacteria is unknown. Here, we analyzed three fiber substrates with diffe...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00819

    authors: Chen M,Fan B,Liu S,Imam KMSU,Xie Y,Wen B,Xin F

    更新日期:2020-05-15 00:00:00

  • Modulation of Lipid Droplet Metabolism-A Potential Target for Therapeutic Intervention in Flaviviridae Infections.

    abstract::Lipid droplets (LDs) are endoplasmic reticulum (ER)-related dynamic organelles that store and regulate fatty acids and neutral lipids. They play a central role in cellular energy storage, lipid metabolism and cellular homeostasis. It has become evident that viruses have co-evolved in order to exploit host lipid metabo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.02286

    authors: Zhang J,Lan Y,Sanyal S

    更新日期:2017-11-28 00:00:00

  • Effect of Menopausal Hormone Therapy on the Vaginal Microbiota and Genitourinary Syndrome of Menopause in Chinese Menopausal Women.

    abstract::Genitourinary syndrome of menopause (GSM) is a chronic and progressive condition with a series of vulvovaginal, sexual, and lower urinary tract discomforts, mainly due to hypoestrogenism. Menopausal hormone therapy (MHT) has generally been considered as the most effective treatment for GSM. In addition, vaginal microb...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.590877

    authors: Geng L,Huang W,Jiang S,Zheng Y,Zhou Y,Zhou Y,Hu J,Li P,Tao M

    更新日期:2020-11-20 00:00:00

  • Pseudomonads Rule Degradation of Polyaromatic Hydrocarbons in Aerated Sediment.

    abstract::Given that the degradation of aromatic pollutants in anaerobic environments such as sediment is generally very slow, aeration could be an efficient bioremediation option. Using stable isotope probing (SIP) coupled with pyrosequencing analysis of 16S rRNA genes, we identified naphthalene-utilizing populations in aerate...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01268

    authors: Wald J,Hroudova M,Jansa J,Vrchotova B,Macek T,Uhlik O

    更新日期:2015-11-19 00:00:00

  • Bacillus subtilis MutS Modulates RecA-Mediated DNA Strand Exchange Between Divergent DNA Sequences.

    abstract::The efficiency of horizontal gene transfer, which contributes to acquisition and spread of antibiotic resistance and pathogenicity traits, depends on nucleotide sequence and different mismatch-repair (MMR) proteins participate in this process. To study how MutL and MutS MMR proteins regulate recombination across speci...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00237

    authors: Carrasco B,Serrano E,Martín-González A,Moreno-Herrero F,Alonso JC

    更新日期:2019-02-13 00:00:00

  • The role of genes domesticated from LTR retrotransposons and retroviruses in mammals.

    abstract::The acquisition of multiple genes from long terminal repeat (LTR) retrotransposons occurred in mammals. Genes belonging to a sushi-ichi-related retrotransposon homologs (SIRH) family emerged around the time of the establishment of two viviparous mammalian groups, marsupials and eutherians. These genes encode proteins ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00262

    authors: Kaneko-Ishino T,Ishino F

    更新日期:2012-07-27 00:00:00

  • Fungal and Bacterial Diversity Isolated from Aquilaria malaccensis Tree and Soil, Induces Agarospirol Formation within 3 Months after Artificial Infection.

    abstract::Aquilaria malaccensis Lam, commonly known as Agarwood, is a highly valuable species used in production of agar oil from its infected wood, which is utilized in pharmaceutical and perfumery industry. Agar oil formation in agarwood takes years through the natural process which is induced by natural or artificial injury ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01286

    authors: Chhipa H,Kaushik N

    更新日期:2017-07-11 00:00:00

  • Co-fermentation Involving Saccharomyces cerevisiae and Lactobacillus Species Tolerant to Brewing-Related Stress Factors for Controlled and Rapid Production of Sour Beer.

    abstract::Increasing popularity of sour beer urges the development of novel solutions for controlled fermentations both for fast acidification and consistency in product flavor and quality. One possible approach is the use of Saccharomyces cerevisiae in co-fermentation with Lactobacillus species, which produce lactic acid as a ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00279

    authors: Dysvik A,La Rosa SL,Liland KH,Myhrer KS,Østlie HM,De Rouck G,Rukke EO,Westereng B,Wicklund T

    更新日期:2020-02-21 00:00:00

  • Glycan cross-feeding activities between bifidobacteria under in vitro conditions.

    abstract::Bifidobacteria colonize the gut of various mammals, including humans, where they may metabolize complex, diet-, and host-derived carbohydrates. The glycan-associated metabolic features encoded by bifidobacteria are believed to be strongly influenced by cross-feeding activities due to the co-existence of strains with d...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01030

    authors: Turroni F,Özcan E,Milani C,Mancabelli L,Viappiani A,van Sinderen D,Sela DA,Ventura M

    更新日期:2015-09-24 00:00:00

  • Biochemical Components Associated With Microbial Community Shift During the Pile-Fermentation of Primary Dark Tea.

    abstract::Primary dark tea is used as raw material for compressed dark tea, such as Fu brick tea, Hei brick tea, Hua brick tea, and Qianliang tea. Pile-fermentation is the key process for the formation of the characteristic properties of primary dark tea, during which the microorganism plays an important role. In this study, th...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01509

    authors: Li Q,Chai S,Li Y,Huang J,Luo Y,Xiao L,Liu Z

    更新日期:2018-07-10 00:00:00

  • Taxonomy and Broad-Spectrum Antifungal Activity of Streptomyces sp. SCA3-4 Isolated From Rhizosphere Soil of Opuntia stricta.

    abstract::Actinobacteria are important producers of bioactive compounds. Extreme ecosystems cause evolution of novel secondary metabolic pathways of Actinobacteria and increase the possible discovery of new biological functions of bioactive compounds. Here, we isolated 65 Actinobacteria from rhizosphere soil samples of Opuntia ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01390

    authors: Qi D,Zou L,Zhou D,Chen Y,Gao Z,Feng R,Zhang M,Li K,Xie J,Wang W

    更新日期:2019-06-28 00:00:00

  • A Single-Nucleotide Deletion in the Transcription Factor Gene bcsmr1 Causes Sclerotial-Melanogenesis Deficiency in Botrytis cinerea.

    abstract::Botrytis cinerea is an important plant pathogenic fungus with a wide range of host. It usually produces black-colored sclerotia (BS) due to deposition of 1,8-dihydroxynaphthalene melanin in sclerotial melanogenesis. Our previous study (Zhou et al., 2018) reported six B. cinerea isolates producing orange-colored sclero...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02492

    authors: Zhou Y,Yang L,Wu M,Chen W,Li G,Zhang J

    更新日期:2017-12-12 00:00:00

  • Nanotechnology: A Valuable Strategy to Improve Bacteriocin Formulations.

    abstract::Bacteriocins are proteinaceous antibacterial compounds, produced by diverse bacteria, which have been successfully used as: (i) food biopreservative; (ii) anti-biofilm agents; and (iii) additives or alternatives to the currently existing antibiotics, to minimize the risk of emergence of resistant strains. However, the...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.01385

    authors: Fahim HA,Khairalla AS,El-Gendy AO

    更新日期:2016-09-16 00:00:00

  • A New Rabbit-Skin Model to Evaluate Protective Efficacy of Tuberculosis Vaccines.

    abstract::Background: BCG protection is suboptimal and there is significant interest to develop new tuberculosis (TB) vaccines. However, there are significant limitations of the current vaccine evaluation systems in the mouse model. Here, we developed a BCG-challenge rabbit skin model as a new way to evaluate the protective eff...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00842

    authors: Chen H,Liu X,Ma X,Wang Q,Yang G,Niu H,Li S,He B,He S,Dannenberg AM Jr,Zhu B,Zhang Y

    更新日期:2017-05-17 00:00:00

  • 6K2-induced vesicles can move cell to cell during turnip mosaic virus infection.

    abstract::To successfully infect plants, viruses replicate in an initially infected cell and then move to neighboring cells through plasmodesmata (PDs). However, the nature of the viral entity that crosses over the cell barrier into non-infected ones is not clear. The membrane-associated 6K2 protein of turnip mosaic virus (TuMV...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00351

    authors: Grangeon R,Jiang J,Wan J,Agbeci M,Zheng H,Laliberté JF

    更新日期:2013-12-04 00:00:00

  • Whole Genome Sequence Analysis of CTX-M-15 Producing Klebsiella Isolates Allowed Dissecting a Polyclonal Outbreak Scenario.

    abstract::Extended-spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae pose an important threat of infection with increased morbidity and mortality, especially for immunocompromised patients. Here, we use the rise of multidrug-resistant K. pneumoniae in a German neurorehabilitation center from April 2015 to April 2016 t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00322

    authors: Becker L,Fuchs S,Pfeifer Y,Semmler T,Eckmanns T,Korr G,Sissolak D,Friedrichs M,Zill E,Tung ML,Dohle C,Kaase M,Gatermann S,Rüssmann H,Steglich M,Haller S,Werner G

    更新日期:2018-02-23 00:00:00